Recombinant Human PDGF R alpha Fc Chimera Protein, CF

Name: Recombinant Human PDGF R alpha Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 6765-PR
Price: 428 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 6765-PR

Citations (4)

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Key Product Details

Source:	NS0
Accession #:	P16234
Applications:	Bioactivity

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Product Specifications for Recombinant Human PDGF R alpha Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human PDGF R alpha protein

Human PDGF R alpha (Met1 - Glu524) Accession # P16234	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gln24

Predicted Molecular Mass

82.8 kDa (monomer)

SDS-PAGE

110-130 kDa, reducing conditions

Activity

Measured by its ability to inhibit the biological activity of PDGF-AB or PDGF-AA using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.

The ED₅₀ for this effect is 0.2-1 μg/mL in the presence of 100 ng/mL recombinant human PDGF-AB.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6765-PR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF R alpha

PDGF R alpha (platelet-derived growth factor receptor alpha) is a type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTK) (1 ‑ 4). PDGF R alpha and PDGF R beta can form homo- or hetero-dimeric receptors when engaged by dimers of the PDGF family of growth factors, which include disulfide-linked homodimers of PDGF-A, B, C or D, or the heterodimer PDGF-AB that is mainly found in human platelets. While multiple in vitro ligand-receptor combinations have been identified, in vivo evidence indicates that PDGF R alpha primarily binds PDGF-AA and PDGF-CC, while PDGF R beta primarily binds PDGF-BB and probably PDGF-DD. Like all class III RTKs, the extracellular domain (ECD) of human PDGF R alpha (aa 24 ‑ 524) contains five immunoglobulin-like domains, while the intracellular region contains a split tyrosine kinase domain (aa 593 ‑ 954) (1 ‑ 4). Within the ECD, human PDGF R alpha shares 85%, 83%, 95%, 93%, and 88% aa sequence identity with mouse, rat, equine, canine and bovine PDGF R alpha respectively. PDGF R alpha autophosphorylates upon dimerization, activating signaling cascades in PI 3-kinase Ras‑MAP kinase, and PLC-gamma pathways (1, 2). Signaling is down‑regulated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. PDGF R alpha is expressed at low levels in most mesenchymal cells, but is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture (1 ‑ 4). During development, mesenchymal cells expressing PDGF R alpha respond to local gradients of epithelially produced PDGF-AA or PDGF-CC during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme (1, 5). Deletion of PDGF R alpha in mice severely impairs mesenchymal derivatives in both embryo and extraembryonic tissues, and high or low PDGF R alpha signaling in humans may result in spina bifida or cleft palate‑type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart and skin (scleroderma) (6 ‑ 8).

Item	Value
References	Andrae, J. et al. (2008) Genes Dev. 22:1276. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917. Matsui, T. et al. (1989) Science 243:800. Klinghoffer, R.A. et al. (2002) Dev. Cell 2:103. Martinho, O. (2009) Br. J. Cancer 101:973. Olson, L.E. and P. Soriano (2009) Dev. Cell 16:303. Baroni, S.S. et al. (2006) N. Engl. J. Med. 354:2667.
Long Name	Platelet-derived Growth Factor Receptor alpha
Entrez Gene IDs	5156 (Human); 18595 (Mouse)
Alternate Names	CD140 antigen-like family member A, CD140a, CD140a antigen, EC 2.7.10, EC 2.7.10.1, MGC74795, PDGF R alpha, PDGF-R-alpha, PDGFR alpha, PDGFR2, PDGFRA, PDGFRA/BCR fusion, RHEPDGFRA, alpha-type platelet-derived growth factor receptor, platelet-derived growth factor receptor, alpha polypeptide, rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein

Citations for Recombinant Human PDGF R alpha Fc Chimera Protein, CF (4)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PDGF R alpha Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Braun et al. (2022), Viral and Cellular Factors Contributing to the Hematogenous Dissemination of Human Cytomegalovirus via Polymorphonuclear Leukocytes Viruses, 14(7).
PMID: 35891541
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Park et al. (2020), Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology PLoS Pathog., 16(6):e1008647.
PMID: 32559251
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Stegmann et al. (2017), A derivative of platelet-derived growth factor receptor alpha binds to the trimer of human cytomegalovirus and inhibits entry into fibroblasts and endothelial cells PLoS Pathog., 13(4):e1006273.
PMID: 28403220
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Wu et al. (2017), Human cytomegalovirus glycoprotein complex gH/gL/gO uses PDGFR-? as a key for entry PLoS Pathog., 13(4):e1006281.
PMID: 28403202

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Showing 1 - 4 of 4 citations Showing All

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Recombinant Human PDGF R alpha Fc Chimera Protein, CF

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