Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

Name: Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF
Brand: R&D Systems
SKU: 10649-OX
Price: 405 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10649-OX

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_003318.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

Product Specifications for Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

Source

Mouse myeloma cell line, NS0-derived human OX40/TNFRSF4 protein

Human OX40/TNFRSF4 (Leu29-Ala216) Accession # NP_003318.1	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu29

Predicted Molecular Mass

47 kDa

SDS-PAGE

60-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human OX40 Ligand/TNFSF4 Protein (Catalog # 1054-OX) is immobilized at 250 ng/mL, the concentration of Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Chimera that produces 50% optimal binding response is found to be approximately 2.5-15 ng/mL.

Scientific Data Examples for Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

	Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Chimera Protein Binding Activity. When Recombinant Human OX40 Ligand/TNFSF4 Protein (1054-OX) is immobilized at 250 ng/mL, the concentration of Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Chimera (Catalog # 10649-OX) that produces 50% optimal binding response is found to be approximately 2.5-15 ng/mL.
	Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human OX40/TNFRSF4 mFc Chimera Protein (Catalog # 10649-OX) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-70 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10649-OX

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Human OX40 is a 48 kDa type I transmembrane glycoprotein with a 28 amino acid (aa) signal sequence, a 185 aa extracellular domain (ECD) that contains a cysteine-rich region, a 20 aa transmembrane segment, and a 41 aa cytoplasmic domain (4). The ECD of human OX40 shares 63% sequence identity with the ECD of mouse and rat OX40. OX40 is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4+ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells’ immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18).

Item	Value
References	Hori, T. (2006) Int. J. Hematol. 83:17. Latza, U. et al. (1994) Eur. J. Immunol. 24:677. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230. Gramaglia, I. et al. (1998) J. Immunol. 161:6510. Xiao, X. et al. (2008) J. Immunol. 181:3193. So, T. and M. Croft (2007) J. Immunol. 179:1427. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907. Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902. Griseri, T. et al. (2010) J. Exp. Med. 207:699. Xiao, X. et al. (2012) J. Immunol. 188:892. Vu, M.D. et al. (2007) Blood 110:2501. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688. Xiao, X. et al. (2012) Nat. Immunol. 13:981. Nakano, M. et al. (2010) Cardiovasc. Res. 88:539. Ishii, N. et al. (2010) Adv. Immunol. 105:63. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757
Long Name	Interleukin 10
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names	CSIF, CSIFMGC126450, Cytokine synthesis inhibitory factor, GVHDS, IL-10, IL-10MGC126451, IL10, IL10A, TGIF, interleukin 10, interleukin-10

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Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

Scientific Data Examples for Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IL-10

Citations for Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

Product Documents for Recombinant Human OX40/TNFRSF4 Mouse IgG2a Fc Protein, CF

Product Datasheets

COA

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