This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

Name: Recombinant Human Osteoprotegerin/TNFRSF11B Protein
Brand: R&D Systems
SKU: 185-OS
Rating: 5 (1 reviews)

The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Recombinant Human Osteoprotegerin/TNFRSF11B Protein

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 185-OS

Citations (12)

(1)

Product Datasheet / COA / SDS

New Version Available - 6945-OS

A New and Improved rh OPG is Now Available! The new protein has ~2 fold better activity!

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	AAB53709.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Osteoprotegerin/TNFRSF11B Protein

Product Specifications for Recombinant Human Osteoprotegerin/TNFRSF11B Protein

Source	Mouse myeloma cell line, NS0-derived human Osteoprotegerin/TNFRSF11B protein Glu22-Leu401
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu22
Predicted Molecular Mass	43.5 kDa (monomer)
SDS-PAGE	55 kDa, reducing conditions
Activity	Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED₅₀ for this effect is 8‑24 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

185-OS

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

185-OS/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Osteoprotegerin/TNFRSF11B

Osteoprotegerin (OPG), also called OCIF (osteoclastogenesis inhibitory factor) is a secreted 55-60 kDa protein that regulates bone density (1-3). As a member of the tumor necrosis factor receptor (TNFR) superfamily of proteins, it is designated TNFRSF11B (1-4). Human OPG cDNA encodes 401 amino acids (aa) including a 21 aa signal peptide and a 380 aa mature soluble protein with four TNFR domains, two death domains and a heparin-binding region (4). The cysteine-rich TNFR domains are essential for ligand interaction, while a cysteine at the C-terminus mediates homodimerization (4). Mature human OPG shares 86%, 87%, 92%, 92% and 88% amino acid sequence identity with mouse, rat, equine, canine and bovine OPG, respectively. OPG is widely expressed and constitutively released as a homodimer by mesenchymal stem cells, fibroblasts and endothelial cells (1, 2, 5, 7). Regulation of its expression by estrogen, parathyroid hormone and cytokines is complex and changes with age (2). OPG has been called a decoy receptor for the TNF superfamily ligands, TRANCE (tumor necrosis factor-related activation-induced cytokine), also called RANK L (receptor activator of NF kappa B ligand), and TRAIL (TNF-related apoptosis-inducing ligand), which also bind TNF family receptors RANK and TRAIL receptors 1-4, respectively (2, 6). TRAIL decreases the release of OPG from cells that express it, while OPG inhibits TRAIL-induced apoptosis (5, 6). Expression of RANK L on the cell surface, and thus its ability to stimulate osteoclastogenesis, is regulated by OPG by intracellular and extracellular mechanisms (7). Within osteoblasts, interaction of the basic domain of OPG with RANK L in the Golgi inhibits RANK L secretion (7). Extracellularly, OPG binding to RANK L results in clathrin-mediated internalization and degradation of both proteins (7, 8). Binding of OPG by syndecan-1 heparin sulfates on multiple myeloma cells also results in OPG internalization and degradation, contributing to bone loss (8, 9). OPG deficiency can cause juvenile Paget’s disease in humans, and insufficient OPG to balance with RANK L and RANK can produce osteoporosis and vascular calcification in both mice and humans (2, 10, 11).

Item	Value
References	Simonet, W.S. et al. (1997) Cell 89:309. Trouvin, A-P. and V. Goeb 2010) Clin. Interv. Aging 5:345. Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:3597. Yamaguchi, K. et al. (1998) J. Biol. Chem. 273:5117. Corallini, F. et al. (2010) J. Cell. Physiol. Dec. 6 [Epub ahead of print]. Emery, J.G. et al. (1998) J. Biol. Chem. 273:14363. Aoki, S. et al. (2010) J. Bone Miner. Res. 25:1907. Tat, S.K. et al. (2006) Bone 39:706. Standal, T. et al. (2002) Blood 100:3002. Whyte, M.P. et al. (2002) N. Engl. J. Med. 347:175. Van Campenhout, A. and J. Golledge (2009) Atherosclerosis 204:321.
Entrez Gene IDs	4982 (Human); 18383 (Mouse)
Alternate Names	OCIF, OCIFMGC29565, OPG, OPGtumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, TNFRSF11B, TR1, tumor necrosis factor receptor superfamily, member 11b

Citations for Recombinant Human Osteoprotegerin/TNFRSF11B Protein (12)

Showing 1 - 10 of 12 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Osteoprotegerin/TNFRSF11B Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Diedisheim et al. (2018), Modeling human pancreatic beta cell dedifferentiation Mol Metab, 0(0).
PMID: 29472102
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

E Vorkapic et al. (2018), Effects of osteoprotegerin/TNFRSF11B in two models of abdominal aortic aneurysms Mol Med Rep, 0(0).
PMID: 29749489
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

CJ Williams et al. (2018), Mutations in osteoprotegerin account for the CCAL1 locus in calcium pyrophosphate deposition disease Osteoarthr. Cartil., 0(0).
PMID: 29578045
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Feng Z et al. (2013), Osteoprotegerin promotes the proliferation of chondrocytes and affects the expression of ADAMTS-5 and TIMP-4 through MEK/ERK signaling. Mol Med Rep, 8(6):1669-79.
PMID: 24126801
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lane D et al. (2013), Osteoprotegerin (OPG) activates integrin, focal adhesion kinase (FAK), and Akt signaling in ovarian cancer cells to attenuate TRAIL-induced apoptosis. J Ovarian Res, 6(1):82.
PMID: 24267510
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Cell Culture Supernates
Applications: Direct ELISA

Mabilleau G et al. (2009), Interleukin-32 promotes osteoclast differentiation but not osteoclast activation. PLoS ONE, 4(1):e4173.
PMID: 19137064
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chamoux E et al. (2008), Osteoprotegerin decreases human osteoclast apoptosis by inhibiting the TRAIL pathway. J. Cell. Physiol., 216(2):536-42.
PMID: 18338379
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Tang CH et al. (2007), Attenuation of bone mass and increase of osteoclast formation in decoy receptor 3 transgenic mice. J. Biol. Chem., 282(4):2346-54.
PMID: 17099218
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bogatkevich GS et al. (2007), Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis. Arthritis Rheum., 56(7):2432-42.
PMID: 17599773
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Secchiero P et al. (2007), Activation of the p53 pathway down-regulates the osteoprotegerin expression and release by vascular endothelial cells. Blood, 111(3):1287-94.
PMID: 18000166
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Locklin RM et al. (2007), Selective targeting of death receptor 5 circumvents resistance of MG-63 osteosarcoma cells to TRAIL-induced apoptosis. Mol. Cancer Ther., 6(12):3219-28.
PMID: 18065493

There are no citations that match your criteria.

Showing 1 - 10 of 12 citations Showing All

Customer Reviews for Recombinant Human Osteoprotegerin/TNFRSF11B Protein (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Human Osteoprotegerin/TNFRSF11B Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 07/19/2016

There are no reviews that match your criteria.

Showing 1 - 1 of 1 review Showing All

FAQs for Recombinant Human Osteoprotegerin/TNFRSF11B Protein

This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Recombinant Human Osteoprotegerin/TNFRSF11B Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Recombinant Human Osteoprotegerin/TNFRSF11B Protein

Formulation, Preparation and Storage

Carrier Free

185-OS

Carrier

185-OS/CF

Carrier Free

Reconstitution Calculator

Background: Osteoprotegerin/TNFRSF11B

Citations for Recombinant Human Osteoprotegerin/TNFRSF11B Protein (12)

Customer Reviews for Recombinant Human Osteoprotegerin/TNFRSF11B Protein (1)

Recombinant Human Osteoprotegerin/TNFRSF11B Protein

FAQs for Recombinant Human Osteoprotegerin/TNFRSF11B Protein

Product Documents for Recombinant Human Osteoprotegerin/TNFRSF11B Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected