Skip to main content

Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF

Catalog # 10059-TA | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
10059-TA-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human OLT-2/TARM1 protein
Human OLT-2/TARM1
(Gln17-Val235)
Accession # NP_001129158.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln17 inferred from enzymatic pyroglutamate treatment revealing Gly18.

Predicted Molecular Mass

50 kDa

SDS-PAGE

64-74 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 2-10 μg/mL.

Scientific Data Images for Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF

Recombinant Human OLT-2/TARM1 Fc Chimera Protein Bioactivity

Recombinant Human OLT-2/TARM1 Fc Chimera Protein Bioactivity

Recombinant Human OLT-2/TARM1 Fc Chimera (Catalog # 10059-TA) inhibits IFN gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 2-10 μg/mL.
Recombinant Human OLT-2/TARM1 Fc Chimera Protein SDS-PAGE

Recombinant Human OLT-2/TARM1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human OLT-2/TARM1 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 64-74 kDa and 130- 150 kDa, respectively.

Formulation, Preparation and Storage

10059-TA
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: OLT-2/TARM1

T cell interacting, activating receptor on myeloid cells-1 (TARM-1), also known as OSCAR-like transcript-2 protein (OLT-2) is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse OLT-2/TARM-1 contains a 242 aa long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of human OLT-2/TARM-1 shares 48% and 49% amino acid (aa) identity with mouse and rat OLT-2/TARM-1 respectively. OLT-2/TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12.OLT-2/TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. OLT-2/TARM-1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. OLT-2/TARM-1 Fc fusion protein inhibits anti-CD3 induced CD4+ cell activation, suggesting that OLT-2/TARM-1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).

References

  1. Radjabova, V., et al. (2015) J Immunol. 195:3149.

Long Name

T-cell-interacting Activating Receptor on Myeloid Cells 1

Alternate Names

OLT2, TARM1

Entrez Gene IDs

441864 (Human); 245126 (Mouse); 499065 (Rat)

Gene Symbol

TARM1

UniProt

Product Documents for Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human OLT-2/TARM1 Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents