Recombinant Human NPRC/NPR3 Fc Chimera Protein, CF

Name: Recombinant Human NPRC/NPR3 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10233-NR

Catalog # 10233-NR

Product Datasheet / COA / SDS

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Key Product Details

Source:	HEK293
Accession #:	P17342.2
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human NPRC/NPR3 Fc Chimera Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human NPRC/NPR3 protein

Human NPRC/NPR3 (Thr24-Glu481) Accession # P17342.2	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Thr24 & Glu46

Predicted Molecular Mass

77 kDa & 75 kDa

SDS-PAGE

78-92 kDa

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human NPRC/NPR3 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Osteocrin (Catalog # 9669-ON) that produces 50% of the optimal binding response is 2-20 ng/mL

Recombinant Human NPRC/NPR3 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human NPRC/NPR3 Fc Chimera Protein Binding Activity When Recombinant Human NPRC/NPR3 Fc Chimera (Catalog # 10233-NR) is immobilized at 2 µg/mL, Recombinant Human Osteocrin (Catalog # 9669-ON) binds with an ED₅₀ of 2-20 ng/mL.
	Recombinant Human NPRC/NPR3 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human NPRC/NPR3 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-92 kDa and 160-200 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10233-NR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: NPRC/NPR3

Atrial Natriuretic Peptide Receptor-3 (NPR3), also known as NPRC or ANPR-C, is one of the three natriuretic peptide receptors (1, 2). Mature human NPR3 is a type I transmembrane glycoprotein that contains a 455 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 37 aa cytoplasmic region. Within the ECD, human NPR3 shares 92% aa sequence identity with mouse and rat NPR3. The natriuretic system is key to the maintenance of vascular tone and cardiovascular homeostasis. It consists of three related natriuretic peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). It also consists of three single-membrane-spanning receptors (NPRs) to mediate the biological activity of these peptides: NPR1, NPR2, and NPR3. NPR1 and NPR2 are guanylyl cyclase receptors that regulate cGMP levels, while NPR3 lacks enzymatic activity and may act as a clearance receptor (1, 2). Both ANP and BNP exhibit high binding affinities to NPR1 and NPR3, while CNP binds with high affinity to NPR2 and NPR3 (2). NPR3 is known to be expressed in the heart, lung, adrenal gland, heart, cerebral cortex, cerebellum, liver and adipocytes and in some cancers (1, 2). Osteocrin was found to be a specific ligand to NPR3 (3). NPR3 is necessary for Osteocrin to regulate femoral, tibial, and metatarsal bone elongation (4).

Item	Value
References	Potter, L.R. et al. (2006) Endocr. Rev. 27:47. Koller, K. J. and D. V. Goeddel (1992) Circulation 86:1081. Thomas, G. et al. (2003) J. Biol. Chem. 278:50563. Moffatt, P. et al. (2007) J. Biol. Chem. 282:36454.
Long Name	Natriuretic Peptide Receptor C
Entrez Gene IDs	4883 (Human); 18162 (Mouse); 25339 (Rat)
Alternate Names	ANP-C, ANPR-C, ANPRCFLJ14054, Atrial natriuretic peptide clearance receptor, Atrial natriuretic peptide receptor type C, C5orf23, GUCY2B, NPR-C, NPR3, NPRC, atrial natriuretic peptide receptor 3, chromosome 5 open reading frame 23, lgj, natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptidereceptor C), stri

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