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Recombinant Human NM23-H2 His-tag Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10466-NM

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10466-NM-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human NM23-H2 protein
Met1-Glu152
with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

18 kDa

SDS-PAGE

19 kDa, under reducing conditions

Activity

Measured by its ability to convert thymidine diphosphate and adenosine triphosphate to thymidine triphosphate and adenosine diphosphate in a coupled assay.
The specific activity is >500,000 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human NM23-H2 His-tag Protein, CF

Recombinant Human NM23-H2 His-tag Protein Enzyme Activity

Recombinant Human NM23-H2 His-tag Protein Enzyme Activity

Recombinant Human NM23-H2 His-tag (Catalog # 10466-NM) is measured by its ability to convert thymidine diphosphate and adenosine triphosphate to thymidine triphosphate and adenosine diphosphate in a coupled assay.
Recombinant Human NM23-H2 His-tag Protein SDS-PAGE

Recombinant Human NM23-H2 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human NM23-H2 His-tag (Catalog # 10466-NM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing a band at 19 kDa under reducing conditions.

Formulation, Preparation and Storage

10466-NM
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: NM23-H2

Non-metastatic Protein 23 Homolog 2 (NM23-H2), also known as Nucleoside diphosphate kinase B (NDPK-B), is a group 1 member of the NDPK family encoded by the NME2 (non metastatic cell) gene (1,2). NDPKs are well-known as housekeeping genes that maintain the balance of nucleoside triphosphate levels by transferring a phosphate group from a nucleoside triphosphate to nucleoside diphosphate. NM23-H2 is a cytosolic protein that forms active hexamers composed of a trimer of dimers (3). The active hexamer contains a "head" region with substrate binding and catalytic activity as well as a Kpn loop and C-terminal extensions that stabilize the structure (3). NM23-H2 (NDPK-B) shares significant homology with NM23-H1 (NDPK-A) at 88% identity (3). In spite of significant homology and catalytic function between these isoforms, the differing amino acids present on the lateral surface of the hexamers lead to specific interactions with other proteins and ultimately result in diverging cellular distribution and function in cells (2).  Both NM23-H2 and NM23-H1 have been reported to act as protein histidine kinases (4-7). NM23-H2 alone has been shown to reversibly histidine phosphorylate beta subunit of trimeric G proteins (4), the Kca3.1 potassium channel (5) and the TRPV5 calcium channel (6) implicating NM23-H2 to have a role in heart failure and activation of B and T cells. Additionally, NM23-H2 has specifically been shown to form a complex with the cystic fibrosis transmembrane conductance regulator (CFTR) (1) to regulate chloride channels. NM23-H2 knockout mice have a defect in potassium channel activation, cytokine production in T cells (8), and have been shown to decrease class switch recombination (CSR) in B cells during an immune response (9).

References

  1. Borthwick, L.A. et al. (2016) PLoS One. 11:e0149097.
  2. Boissan, M. et al. (2018) Lab. Invest. 98:164.
  3. Webb, P.A. et al. (1995) J. Mol. Biol. 251:574.
  4. Cuello, F. et al. (2003) J. Biol. Chem. 278:7220.
  5. Srivastava, S. et al. (2006) Mol. Cell. 24:665.
  6. Cai, X. et al. (2014) Mol. Bol. Cell 25:1244.
  7. Attwood, P.V. et al. (2018) Lab. Invest. 98:283.
  8. Di, L. et al. (2010) J. Biol. Chem. 285:38765.
  9. Zheng, S. et al. (2019) FEBS Lett. 593:80.

Long Name

Non-metastatic Protein 23 Homolog 2

Alternate Names

NDK B, NDP Kinase B2, NDPKB, NM23B, NME2

Entrez Gene IDs

4831 (Human); 18103 (Mouse); 83782 (Rat)

Gene Symbol

NME2

UniProt

Additional NM23-H2 Products

Product Documents for Recombinant Human NM23-H2 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human NM23-H2 His-tag Protein, CF

For research use only