Product Specifications for Recombinant Human NKp30/NCR3 His-tag Protein, CF
Human embryonic kidney cell, HEK293-derived human NKp30/NCR3 protein Leu19-Thr138, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
21-33 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 Fc Chimera (Catalog # 7144-B7) is immobilized at 4 µg/mL (100 µL/well), Recombinant Human NKp30/NCR3 His-tag (Catalog # 11271-NK) binds with an ED50 of 0.600-7.20 µg/mL.
Scientific Data Examples for Recombinant Human NKp30/NCR3 His-tag Protein, CF
Recombinant Human NKp30/NCR3 His-tag Protein Binding Activity.
When Recombinant Human B7-H6 Fc Chimera Protein (7144-B7) is immobilized at 4 µg/mL (100 µL/well), Recombinant Human NKp30/NCR3 His-tag Protein (Catalog # 11271-NK) binds with an ED50 of 0.600‑7.20 µg/mL.
Recombinant Human NKp30/NCR3 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human NKp30/NCR3 His-tag Protein (Catalog # 11271-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 21-33 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK-mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM‑bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.
Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
Pende, D. et al. (1999) J. Exp. Med. 190:1505.
Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.
Citations for Recombinant Human NKp30/NCR3 His-tag Protein, CF
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Product Documents for Recombinant Human NKp30/NCR3 His-tag Protein, CF
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