Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Name: Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10511-NK
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10511-NK

Product Datasheet / COA / SDS

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Key Product Details

Source:	HEK293
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Product Specifications for Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human NKG2A/CD159a protein

MD	Human IgG1 (Pro100-Lys330)	IEGR	Human NKG2A/CD159a (Pro94-Leu233) Accession # P26715.2
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met

Predicted Molecular Mass

43 kDa

SDS-PAGE

55-65 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CD94 His-tag (Catalog # 9270-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human NKG2A/CD159a Fc Chimera (Catalog # 10511-NK) binds with an ED₅₀ of 2-16 µg/mL.

Scientific Data Examples for Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

	Recombinant Human NKG2A/CD159a Fc Chimera Protein Binding Activity. When Recombinant Human CD94 His-tag (9270-CD) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human NKG2A/CD159a Fc Chimera (Catalog # 10511-NK) binds with an ED₅₀ of 2-16 µg/mL.
	Recombinant Human NKG2A/CD159a Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human NKG2A/CD159a Fc Chimera Protein (10511-NK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-65 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10511-NK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKG2A/CD159a

Killer cell lectin like receptor C1 (KLRC1), also known as NKG2A/NKG2B type II integral membrane protein or NK cell receptor A, is a member of the C-type lectin family (1). The NKG2 family is comprised of three unique receptor isoforms, NKG2A, NKG2C and NKG2E, which regulate both inhibitory and activating signals and are attractive targets for immune-checkpoint inhibition (1-3). Mature human NKG2A consists of a cytoplasmic domain containing two immunoreceptor tyrosine based inhibitory motifs (ITIM), a helical type II transmembrane domain, and an extracellular domain (ECD) with a C-type lectin motif (4). Within the mature ECD, human NKG2A shares 41% and 45% amino acid sequence identity with mouse and rat NKG2A, respectively. NKG2A is an inhibitory member of the NKG2 family and is expressed on CD56high natural killer (NK) cells, natural killer T (NKT) cells and a subset of CD8+ alpha beta T cells. NKG2A forms a disulfide linked, dimeric complex with another C-type lectin, CD94 (KLRD1), and functions by binding with HLA-E, a non-classical MHC I molecule (1-4). The increased expression of NKG2A in tumor‑infiltrating NK cells is also emerging as a contributor in determining the poor prognosis of hepatocellular, lung or other carcinomas and in some cases may be a predictive factor of tumor metastasis (5). NKG2A immune checkpoint blockade in combination with PD-1/PD-L1 blockade or therapeutic cancer vaccines may hold promise in the treatment or multiple myeloma (MM) and various other solid and blood tumors or may improve the clinical outcome of patients who have undergone allogeneic hematopoietic stem cell transplantation (5-9).

Item	Value
References	Haanen, J. and Cerundolo, V. (2018) Cell. 175:1720. Ceelan, B.C. and Antonia, S.J. (2019) Antonia Nature Reviews Clinical Oncology 16:277. Montfoort, N. et al. (2018) Cell. 175:1744. Brooks, A. et al. (1997) J. Exp. Med. 185:795. Zaghi, E. et al. (2019) J Leukoc Biol. 105:1243. van Hall, T. et al. (2019) J Immunother Cancer. 7:263. André, P. et al. (2019) Cell. 175:1731. Mahaweni, N.M. et al. (2018) Front Immunol. 9:2848. Borst, L. et al. (2020) doi: 10.1158/1078-0432.CCR-19-2095.
Long Name	Natural Killer G2A
Entrez Gene IDs	3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)
Alternate Names	C-lectin type II protein, CD159 antigen-like family member A, CD159a, CD159a antigen, Klrc1, MGC13374, NK cell receptor A, NKG2, NKG2-1/B activating NK receptor, NKG2-A, NKG2-A/B type II integral membrane protein, NKG2-A/B-activating NK receptor, NKG2-A/NKG2-B type II integral membrane protein, NKG2-B, NKG2A, NKG2AMGC59791, killer cell lectin-like receptor subfamily C, member 1, natural killer cell lectin, natural killer group protein 2

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Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: NKG2A/CD159a

Citations for Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Product Documents for Recombinant Human NKG2A/CD159a Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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