Recombinant Human MSP/MST1 Protein

Name: Recombinant Human MSP/MST1 Protein
Brand: R&D Systems
SKU: 352-MS
Rating: 4 (1 reviews)

Catalog # 352-MS | R&D Systems, Inc. a Bio-Techne Brand

(1)

Citations (8)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	AAA59872
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human MSP/MST1 Protein

Technical Data
Citations (8) / Reviews (1)

Product Specifications for Recombinant Human MSP/MST1 Protein

Source	Mouse myeloma cell line, NS0-derived human MSP/MST1 protein Gln19-Gly711
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained, Gln19 predicted & Val484
Predicted Molecular Mass	78.5 kDa (MSP alpha / beta ); 53.3 kDa ( alpha chain), 25.2 kDa ( beta chain)
SDS-PAGE	78.5 kDa (MSP alpha / beta ); 53.3 kDa ( alpha chain), 25.2 kDa ( beta chain)
Activity	Measured by its ability to activate MSP R/Ron in MDA-MB-453 or T47D human breast cancer cells. 80 ng/mL of the recombinant human MSP significantly induces phosphorylation of MSP R/Ron measured by DuoSetIC human phospho-MSP R/Ron kit (Catalog # DYC1947). Measured by its binding ability in a functional ELISA. When Recombinant Human MSPR/Ron Protein (Catalog #1947-MS) is represent at 100 ng/mL (100 µg/mL), The concentration of Recombinant Human MSP/MST1 that produces 50% optimal binding response is found to be 8-40 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

352-MS

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

352-MS/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: MSP/MST1

Macrophage stimulating protein (MSP), also known as HGF-like protein, and scatter factor-2, is a member of the HGF family of growth factors (1). MSP is secreted as an inactive single chain precursor (pro-MSP) that contains a PAN/APPLE-like domain, four kringle domains, and a peptidase S1 domain which lacks enzymatic activity (2). Human MSP shares 79% aa sequence identity with mouse MSP and 44% aa sequence identity with human HGF. Pro-MSP is secreted by hepatocytes under the positive and negative control of CBP in complex with either HNF-4 or RAR, respectively (3). Circulating pro-MSP is proteolytically cleaved in response to tissue injury to yield biologically active disulfide linked heterodimers consisting of a 45 - 62 kDa alpha and a 25-35 kDa beta chain (4, 5). Pro-MSP can be activated by MT-SP1, a transmembrane protease that is expressed on macrophages and is upregulated in many cancers (6). Heterodimeric MSP as well as the isolated beta chain bind to MSP R/Ron with high-affinity, although only heterodimeric MSP can induce receptor dimerization and signaling (7, 8). MSP induces macrophage and keratinocyte proliferation and osteoclast activation (9, 10). It also inhibits LPS- or IFN-induced iNOS and IL-12 expression by macrophages and prevents apoptosis of epithelial cells separated from the ECM (11, 12).

Item	Value
References	Wang, M.-H. et al. (2002) Scand. J. Immunol. 56:545. Han, S. et al. (1991) Biochemistry 30:9768. Muraoka, R.S. et al. (1999) Endocrinology 140:187. Wang, M.-H. et al. (1996) J. Clin. Invest. 97:720. Nanney, L.B. et al. (1998) J. Invest. Dermatol. 111:573. Bhatt, A.S. et al. (2007) Proc. Natl. Acad. Sci. 104:5771. Wang, M.-H. et al. (1997) J. Biol. Chem. 272:16999. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937. Wang, M.-H. et al. (1996) Exp. Cell Res. 226:39. Kurihara, N. et al. (1998) Exp. Hematol. 26:1080. Morrison, A.C. et al. (2004) J. Immunol. 172:1825. Liu, Q.P. et al. (1999) J. Immunol. 163:6606.
Long Name	Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Entrez Gene IDs	4485 (Human); 15235 (Mouse); 24566 (Rat)
Alternate Names	D3F15S2, EC 3.4.21, HGFL, HGFLhepatocyte growth factor-like protein, MSP, MSPDNF15S2, MST1, Macrophage stimulatory protein, Macrophage-stimulating protein, NF15S2, SF2, hepatocyte growth factor-like protein homolog, macrophage stimulating 1 (hepatocyte growth factor-like)

Citations for Recombinant Human MSP/MST1 Protein (8)

Showing 1 - 8 of 8 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human MSP/MST1 Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

A Khaibullin et al. (2018-03-08), RON kinase inhibition reduces renal endothelial injury in sickle cell disease mice Haematologica, 2018-00(0).
PMID: 29519868
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

GI Vladimer et al. (2017-04-24), Global survey of the immunomodulatory potential of common drugs Nat. Chem. Biol., 2017-00(0).
PMID: 28437395
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

B Yin et al. (2017-04-19), RON and c-Met facilitate metastasis through the ERK signaling pathway in prostate cancer cells Oncol. Rep., 2017-00(0).
PMID: 28440432
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Yang C et al. (2015-02-06), Small heterodimer partner interacts with NLRP3 and negatively regulates activation of the NLRP3 inflammasome. Nat Commun, 2015-06(0):6115.
PMID: 25655831
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhou D et al. (2015-02-04), Variant RONDelta160 of the RON receptor tyrosine kinase promotes the growth and invasion in vitro and in vivo in gastric cancer cell lines. Cancer Cell Int, 2015-015(1):9.
PMID: 25685065
Species: Human

Applications: Enzyme Assay Substrate

Bhatt AS et al. (2007-03-27), Coordinate expression and functional profiling identify an extracellular proteolytic signaling pathway. Proc. Natl. Acad. Sci. U.S.A., 2007-0104(14):5771-6.
PMID: 17389401
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gunella G et al. (2006-04-24), Macrophage-stimulating protein differently affects human alveolar macrophages from smoker and non-smoker patients: evaluation of respiratory burst, cytokine release and NF-kappaB pathway. Br. J. Pharmacol., 2006-0148(4):478-89.
PMID: 16633352
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rampino T et al. (2002-03-01), Macrophage-stimulating protein is produced by tubular cells and activates mesangial cells. J. Am. Soc. Nephrol., 2002-013(3):649-57.
PMID: 11856768

There are no citations that match your criteria.

Showing 1 - 8 of 8 citations Showing All

Customer Reviews for Recombinant Human MSP/MST1 Protein (1)

4 out of 5

1 customer rating

5 stars

4 stars

100%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Recombinant Human MSP/MST1 Protein

Name: Anonymous

Application: Immunoassay Standard

Verified Customer | Posted 09/23/2019

We used this protein for developing an ELISA in combination with mAb (MAB352) and pAb (AF352). This combination worked well and detected human MSP in serum samples efficiently.

Recombinant Human MSP/MST1 Protein

Catalog # 352-MS | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Specifications for Recombinant Human MSP/MST1 Protein

Formulation, Preparation and Storage

Carrier Free

352-MS

Carrier

352-MS/CF

Carrier Free

Reconstitution Calculator

Background: MSP/MST1

Citations for Recombinant Human MSP/MST1 Protein (8)

Customer Reviews for Recombinant Human MSP/MST1 Protein (1)

Customer Images

Recombinant Human MSP/MST1 Protein

Product Documents for Recombinant Human MSP/MST1 Protein

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected