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Recombinant Human MSPR/Ron Protein, CF

Catalog # 1947-MS | R&D Systems, Inc. a Bio-Techne Brand
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1947-MS-050

Key Product Details

Accession #

Source

NS0

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human MSP R/Ron protein
Glu25-Leu571 (single chain) & Gly310-Leu571 (beta), with a C-terminal 10-His tag; Glu25-Arg309 (alpha)

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu25 & Gly310

Predicted Molecular Mass

60 kDa (single chain), 29.7 kDa ( alpha subunit), 29.6 kDa ( beta subunit)

SDS-PAGE

75 kDa, 40 kDa and 38 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human MSP R/Ron is immobilized at 100 ng/mL (100 μL/well), the concentration of recombinant human MSP/MST1 that produces 50% optimal binding response is found to be 8-40 ng/mL.

Formulation, Preparation and Storage

1947-MS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MSPR/Ron

Macrophage stimulating protein receptor (MSP R), encoded by the human Ron and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea (1, 2). This family of receptors is synthesized as a single-chain precursors that are cleaved into a mature disulfide-linked heterodimers composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Biologically active ligands (MSP and HGF) for this family of receptors are also disulfide-linked alpha-beta heterodimers. Human MSP R cDNA encodes a 1400 amino acid (aa) residue precursor protein with a 24 aa signal peptide, a 285 aa residue alpha chain (Glu25-Arg309) and a 1091 aa residue transmembrane beta chain (Gly310-Thr1400). The extracellular domain of MSP R is comprised of an N-terminal sema domain, a PSI (plexin semaphorins integrins) domain, followed by four immunoglobulin-like folds shared by plexins and transcription factors (3). The soluble sema domain binds MSP and inhibits the MSP R-dependent signaling pathways. MSP receptor is expressed in multiple tissues including specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage (1, 2). Although free MSP alpha or beta chains have been shown to bind MSP R, only the heterodimeric MSP can induce receptor activation and cause biological activity (4, 5). MSP R associates with other transmembrane molecules including integrins, cadherins and other cytokine receptors. Transactivation and signaling crosstalk between MSP R and its associated transmembrane receptors have been demonstrated (6-8). Human MSP R shares 72% amino acid sequence identity with mouse MSP R.

References

  1. Gaudino, G. et al. (1994) EMBO J. 13:3524.
  2. Wang, M-H. et al. (1994) Science 266:117.
  3. Angelonis, D. et al. (2004) J. Biol. Chem. 279:3726.
  4. Wang, M-H. et al. (1997) J. Biol. Chem. 272:16999.
  5. Danilkovitch, A. et al. (1999) J. Biol. Chem. 274:29937.
  6. Danilkovitch-Miagkova, A. et al. (2000) J. Biol. Chem. 275:14783.
  7. Danilkovitch-Miagkova, A. and A. Leonard (2001) Histol. Histopathol. 16:623.
  8. Santora, M. et al. (2003) Devel. Cell 2:257.

Long Name

Macrophage Stimulating Protein Receptor

Alternate Names

CD136, MSP R, MST1R, Ron

Entrez Gene IDs

4486 (Human); 19882 (Mouse)

Gene Symbol

MST1R

UniProt

Product Documents for Recombinant Human MSPR/Ron Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human MSPR/Ron Protein, CF

For research use only

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    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 10/24/2017
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