Recombinant Human/Mouse Wnt-5a Protein

Name: Recombinant Human/Mouse Wnt-5a Protein
Brand: R&D Systems
SKU: 645-WN
Rating: 5 (8 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 645-WN

Citations (165)

(8)

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Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	P22725
Applications:	Bioactivity

View Full Technical Details for Recombinant Human/Mouse Wnt-5a Protein

Product Specifications for Recombinant Human/Mouse Wnt-5a Protein

Source	Chinese Hamster Ovary cell line, CHO-derived Wnt-5a protein Gln38-Lys380
Purity	>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Asn44 & No results obtained: Gln38 predicted
Predicted Molecular Mass	38 kDa
SDS-PAGE	45 kDa, reducing conditions
Activity	Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a. Optimal concentrations should be determined by each laboratory for each application.

Scientific Data Examples for Recombinant Human/Mouse Wnt-5a Protein

	Bioactivity of Mouse Wnt-5a Recombinant mouse Wnt-5a (645-WN) inhibits Wnt-3a-induced alkaline phosphatase production in the MC3T3-E1 mouse preosteoblast cell line. The ED₅₀ for this effect is 0.1-0.5 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a (1324-WN).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

645-WN

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

645-WN/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-5a

Wnt-5a is a 44‑50 kDa member of the Wnt family of proteins (1‑6). Based on its activity towards C57Mg mammary epithelium, it is classified as a nontransforming Wnt. Human Wnt‑5a is synthesized as a 380 amino acid (aa) precursor that contains a 37 aa signal sequence, a 25 aa prosegment, and a 319 aa mature region (1, 2, 3). The mature region has 24 cysteine residues that form multiple intrachain disulfide bonds, plus four N‑linked glycosylation sites that are utilized for proper secretion (3, 5, 7). There is also a palmitate adduct at Cys104 that is essential for activity, and a potential palmitoleic acid modification at Ser244 that may also contribute to secretion (7‑9). One alternative start site is reported at Met16. Over aa 38‑380, human and mouse Wnt‑5a are identical in amino acid sequence (1, 10). Cells known to express Wnt‑5a include brainstem astrocytes (11), mammary epithelium (12), CD34+ primitive progenitor stem cells (13), chondrocytes (14), CD34^- pericytes and vascular smooth muscle cells (15), plus mesenchymal cells at various sites (16, 17). There are multiple receptors for Wnt‑5a. These include Fzd-1, -2,
-3, -4, -5, and -7 (3, 18‑22), Ror2 (3), LRP6 (23), Ryk (24) and sFRP1 (25). All these molecules function within the context of a larger number of “co‑factors” that regulate signaling by the Wnts. Initially, it was suggested that there were three pathways for Wnt signaling; a beta -catenin-mediated canonical pathway, and two noncanonical pathways described as the Wnt/JNK (PCP) pathway and the Wnt/Ca⁺⁺ pathway (26, 27). And it was assumed that various Wnts could be accommodated by these classifications. At present, it is now recognized that individual Wnts, through various combinations of receptor complex subunits, can have diverse effects, perhaps even within the same cell (3, 6, 27). Further complexity is introduced by the fact that Xenopus Wnt‑5a and Wnt‑11 are known to form bioactive heterodimers following Tyr sulfation (28). Thus, predicting the activity of Wnt‑5a, or any other Wnt, on any cell type will require substantial insight into the interaction between all the extracellular, cell surface and intracellular components of the Wnt signaling system.

Item	Value
References	Clark, C.C. et al. (1993) Genomics 18:249. LeJeune, S. et al. (1995) Clin. Cancer Res. 1:215. Mikels, A.J. & R. Nusse (2006) PLoS Biol. 4:e115. Nishita, M. et al. (2010) Trends Cell Biol. 20:346. Mikels, A.J. & R. Nusse (2006) Oncogene 25:7461. van Amerongen, R. & R. Nusse (2009) Development 136:3205. Kurayoshi, M. et al. (2007) Biochem. J. 402:515. Takada, R. et al. (2006) Dev. Cell 11:791. Port, F. & K. Basler (2010) Traffic May 3. [Epub ahead of print]. Gavin, B.J. et al. (1990) Genes Dev. 4:2319. Castelo-Branco, G. et al. (2006) Mol. Cell. Neurosci. 31:251. Jonsson, M. et al. (1998) Br. J. Cancer 78:430. van Den Berg, D.J. et al. (1998) Blood 92:3189. Kruger, C. & C. Kappen (2010) PLoS One 5:e8978. Lin, G. et al. (2008) Stem Cells Dev. 17:1053. Lickert, H. et al. (2001) Mech. Dev. 105:181. Danielson, K.G. et al. (1995) J. Biol. Chem. 270:31225. Gazit, A. et al. (1999) Oncogene 18:5959. Bazhin, A. V. et al. (2010) Cell. Mol. Life Sci. 67:817. Kawasaki, A. et al. (2007) Cell. Signal. 19:2498. Blumenthal, A. et al. (2006) Blood 108:965. Umbhauer, M. et al. (2000) EMBO J. 19:4944. Bryja, V. et al. (2009) Mol. Biol. Cell 20:924. Keeble, T.R. et al. (2006) J. Neurosci. 26:5840. Lin, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11196. Rao, T.P. & M. Kuhl (2010) Circ. Res. 106:1798. McDonald, S.L. & A. Silver (2009) Br. J. Cancer 101:209. Cha, S-W. et al. (2009) Curr. Biol. 19:1573.
Long Name	Wingless-type MMTV Integration Site Family, Member 5a
Entrez Gene IDs	7474 (Human); 22418 (Mouse)
Alternate Names	WNT-5A protein, Wnt-5a, Wnt5a, hWNT5A, protein Wnt-5a, wingless-type MMTV integration site family, member 5A

Citations for Recombinant Human/Mouse Wnt-5a Protein (165)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human/Mouse Wnt-5a Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2102), Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex. J. Cell. Sci., 125(0):2017-29.
PMID: 22328498
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Ashry et al. (2022), FSH stimulated bovine granulosa cell steroidogenesis involves both canonical and noncanonical WNT signaling Domestic animal endocrinology, 78(0):106678.
PMID: 34607220
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Dehghani-G et al. (2022), Wnt5A and TGFbeta1 Converges through YAP1 Activity and Integrin Alpha v Up-Regulation Promoting Epithelial to Mesenchymal Transition in Ovarian Cancer Cells and Mesothelial Cell Activation Cells, 11(2).
PMID: 35053353
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Zhao et al. (2022), Cadmium exposure reprograms energy metabolism of hematopoietic stem cells to promote myelopoiesis at the expense of lymphopoiesis in mice Oncogene, 231(0):113208.
PMID: 35051759
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

EM Ghia et al. (2022), High expression level of ROR1 and ROR1-signaling associates with venetoclax resistance in chronic lymphocytic leukemia Leukemia, 0(0).
PMID: 35418613
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

CJ Brereton et al. (2022), Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosis Elife, 11(0).
PMID: 35188460
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

C Kornsuthis et al. (2022), Non-canonical Wnt signaling participates in Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells Scientific Reports, 12(1):7583.
PMID: 35534526
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

X Xue et al. (2022), Transient and Prolonged Activation of Wnt Signaling Contribute Oppositely to the Pathogenesis of Asherman's Syndrome International Journal of Molecular Sciences, 23(15).
PMID: 35955940
Species: Mouse
Sample Types: Whole Cells
Applications: Differentiation

N Salerno et al. (2022), Pharmacological clearance of senescent cells improves cardiac remodeling and function after myocardial infarction in female aged mice Mechanisms of Ageing and Development, 208(0):111740.
PMID: 36150603
Species: Porcine
Sample Types: Whole Cells
Applications: Bioassay

LJ Spicer et al. (2022), Hormone regulation of thrombospondin-1 mRNA in porcine granulosa cells in vitro Oncogene, 244(0):107048.
PMID: 35914333
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

KB Kim et al. (2022), WNT5A-RHOA signaling is a driver of tumorigenesis and represents a therapeutically actionable vulnerability in small cell lung cancer Cancer Research, 0(0).
PMID: 36102736
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LV Lopez et al. (2022), MicroRNA Expression in the Infarcted Heart Following Neonatal Cardiovascular Progenitor Cell Transplantation in a Sheep Model of Stem Cell-Based Repair Cell Transplantation, 31(0):9636897221136.
PMID: 36564913
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Z Zhang et al. (2021), Regulation of ABCG2 expression by Wnt5a through FZD7 in human pancreatic cancer cells Mol Med Rep, 23(1).
PMID: 33200805
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Wan et al. (2021), FOSL2 promotes VEGF-independent angiogenesis by transcriptionnally activating Wnt5a in breast cancer-associated fibroblasts Theranostics, 11(10):4975-4991.
PMID: 33754039
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

T Skaria et al. (2021), Transcriptional Regulation of Drug Metabolizing CYP Enzymes by Proinflammatory Wnt5A Signaling in Human Coronary Artery Endothelial Cells Frontiers in Pharmacology, 12(0):619588.
PMID: 34079452
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

E Ramos-Fern et al. (2021), Wnt5a promotes hippocampal postsynaptic development and GluN2B-induced expression via the eIF2&alpha HRI kinase Scientific Reports, 11(1):7395.
PMID: 33795747
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Tai et al. (2021), IgD promotes pannus formation by activating Wnt5A-Fzd5-CTHRC1-NF-kappaB signaling pathway in FLS of CIA rats and the regulation of IgD-Fc-Ig fusion protein International immunopharmacology, 101(0):108261.
PMID: 34688134
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Radaszkiew et al. (2021), RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy Elife, 10(0).
PMID: 34702444
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Feng et al. (2021), Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation Science Advances, 7(20).
PMID: 33990333
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Jurásek et al. (2021), Phosphorylation-induced changes in the PDZ domain of Dishevelled 3 Scientific Reports, 11(1):1484.
PMID: 33452274
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Kowalski-J et al. (2021), Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain Science Advances, 7(46):eabj7917.
PMID: 34757789
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Neuhaus et al. (2021), Immunocytochemical Analysis of Endogenous Frizzled-(Co-)Receptor Interactions and Rapid Wnt Pathway Activation in Mammalian Cells International Journal of Molecular Sciences, 22(21).
PMID: 34769487
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: Bioassay

A Böttcher et al. (2021), Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates Nature Cell Biology, 23(1):23-31.
PMID: 33398177
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

NC DeVito et al. (2021), Pharmacological Wnt ligand inhibition overcomes key tumor-mediated resistance pathways to anti-PD-1 immunotherapy Cell Reports, 35(5):109071.
PMID: 33951424
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

P Kozielewic et al. (2020), Structural insight into small molecule action on Frizzleds Nat Commun, 11(1):414.
PMID: 31964872
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Luo et al. (2020), H3K27me3-mediated PGC1&alpha gene silencing promotes melanoma invasion through WNT5A and YAP J. Clin. Invest., 130(2):853-862.
PMID: 31929186
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

E Flores-Her et al. (2020), Canonical and non-canonical Wnt signaling are simultaneously activated by Wnts in colon cancer cells Cell. Signal., 72(0):109636.
PMID: 32283254
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

SY Jeong et al. (2020), Ryk modulates the niche activity of mesenchymal stromal cells by fine-tuning canonical Wnt signaling Exp. Mol. Med., 0(0).
PMID: 32724069
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

W Kawarazaki et al. (2020), Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency J. Clin. Invest., 0(0).
PMID: 32597829
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Liu et al. (2020), Wnt5a/CaMKII/ERK/CCL2 axis is required for tumor-associated macrophages to promote colorectal cancer progression Int. J. Biol. Sci., 16(6):1023-1034.
PMID: 32140070
Species: Rat

Applications: Cell Culture

M Mecha et al. (2020), Involvement of Wnt7a in the role of M2c microglia in neural stem cell oligodendrogenesis J Neuroinflammation, 17(1):88.
PMID: 32192522
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

M Díaz-Corán et al. (2020), Norrin restores blood-retinal barrier properties after vascular endothelial growth factor-induced permeability J. Biol. Chem., 0(0).
PMID: 32086377
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

B Theivanthi et al. (2020), A tumor-intrinsic PD-L1-NLRP3 inflammasome signaling pathway drives resistance to anti-PD-1 immunotherapy J. Clin. Invest., 0(0).
PMID: 32017708
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

X Qiao et al. (2020), Wnt5a regulates Ameloblastoma Cell Migration by modulating Mitochondrial and Cytoskeletal Dynamics J Cancer, 11(18):5490-5502.
PMID: 32742496
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Karvonen et al. (2020), Glucocorticoids induce differentiation and chemoresistance in ovarian cancer by promoting ROR1-mediated stemness Cell Death Dis, 11(9):790.
PMID: 32989221
Species: Human
Sample Types: Whole Cells
Applications: Control

Q Chen et al. (2020), Egr-1 mediates low-dose arecoline induced human oral mucosa fibroblast proliferation via transactivation of Wnt5a expression BMC Mol Cell Biol, 21(1):80.
PMID: 33167868
Species: Mouse
Sample Types: Whole Tissue
Applications: Tissue Culture

C Morenilla- et al. (2020), A Zic2-regulated switch in a noncanonical Wnt/&betacatenin pathway is essential for the formation of bilateral circuits Sci Adv, 6(46).
PMID: 33188033
Species: Mouse
Sample Types: Reference Standard
Applications: ELISA Standard

S Ikezu et al. (2020), Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities Mol. Psychiatry, 0(0).
PMID: 32071385
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

A Reggio et al. (2020), Adipogenesis of skeletal muscle fibro/adipogenic progenitors is affected by the WNT5a/GSK3/beta-catenin axis Cell Death Differ., 0(0).
PMID: 32382110
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Bose et al. (2020), Rlip Depletion Suppresses Growth of Breast Cancer Cancers (Basel), 12(6).
PMID: 32498332
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Qiao et al. (2020), Wnt5a regulates Ameloblastoma Cell Migration by modulating Mitochondrial and Cytoskeletal Dynamics J Cancer, 11(18):5490-5502.
PMID: 32742496
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Wei et al. (2020), Notch signalling drives synovial fibroblast identity and arthritis pathology Nature, 582(7811):259-264.
PMID: 32499639
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Zhang et al. (2019), Inhibition of chemotherapy resistant breast cancer stem cells by a ROR1 specific antibody Proc. Natl. Acad. Sci. U.S.A., 116(4):1370-1377.
PMID: 30622177
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Lutze et al. (2019), Non-canonical WNT-signaling controls differentiation of lymphatics and extension lymphangiogenesis via RAC and JNK signaling Sci Rep, 9(1):4739.
PMID: 30894622
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Karvonen et al. (2019), Wnt5a and ROR1 activate non-canonical Wnt signaling via RhoA in TCF3-PBX1 acute lymphoblastic leukemia and highlight new treatment strategies via Bcl-2 co-targeting Oncogene, 0(0).
PMID: 30631148
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Kaiser et al. (2019), WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis Nat Commun, 10(1):1498.
PMID: 30940800
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Ji et al. (2019), Systematically understanding the immunity leading to CRPC progression PLoS Comput. Biol., 15(9):e1007344.
PMID: 31504033
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Wu et al. (2019), Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors Cells, 8(10).
PMID: 31557955
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

G Barbero et al. (2019), An Autocrine Wnt5a Loop Promotes NF-kappaB Pathway Activation and Cytokine/Chemokine Secretion in Melanoma Cells, 8(9).
PMID: 31510045
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MM de Rezende et al. (2019), Divergent effects of Wnt5b on IL-3- and GM-CSF-induced myeloid differentiation Cell. Signal., 67(0):109507.
PMID: 31857239
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

F Zhao et al. (2019), Construction of a vascularized bladder with autologous adipose-derived stromal vascular fraction cells combined with bladder acellular matrix via tissue engineering J Tissue Eng, 10(0):2041731419891.
PMID: 31827758
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

BK Tusi et al. (2018), Population snapshots predict early haematopoietic and erythroid hierarchies Nature, 0(0).
PMID: 29466336
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Kokabu et al. (2018), BMP3 expression by osteoblast lineage cells is regulated by canonical Wnt signaling FEBS Open Bio, 8(2):168-176.
PMID: 29435407
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BD Boyan et al. (2018), Role of Wnt11 during Osteogenic Differentiation of Human Mesenchymal Stem Cells on Microstructured Titanium Surfaces Sci Rep, 8(1):8588.
PMID: 29872092
P Qian et al. (2018), Retinoid-Sensitive Epigenetic Regulation of the Hoxb Cluster Maintains Normal Hematopoiesis and Inhibits Leukemogenesis Cell Stem Cell, 22(5):740-754.e7.
PMID: 29727682
Sample Types: Whole Cells
Applications: Bioassay

HK Lin et al. (2018), Caveolin-1 down-regulation is required for Wnt5a-Frizzled 2 signalling in Ha-RasV12-induced cell transformation J. Cell. Mol. Med., 0(0).
PMID: 29502342
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BN Nakamura et al. (2018), A20 regulates canonical wnt-signaling through an interaction with RIPK4 PLoS ONE, 13(5):e0195893.
PMID: 29718933
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay

T Takahashi et al. (2018), The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice Int J Mol Sci, 19(3).
PMID: 29510587
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Xiong et al. (2018), miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer Cell. Signal., 44(0):33-42.
PMID: 29339084
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Xu et al. (2018), Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells Stem Cells, 0(0).
PMID: 29377497
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

YK Bae et al. (2018), Intracellular Calcium Determines the Adipogenic Differentiation Potential of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells via the Wnt5a/?-Catenin Signaling Pathway Stem Cells Int, 2018(0):6545071.
PMID: 30123291
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

SH Lee et al. (2018), Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes Nat Commun, 9(1):3434.
PMID: 30143647
Species: Mouse

P Andersen et al. (2018), Precardiac organoids form two heart fields via Bmp/Wnt signaling Nat Commun, 9(1):3140.
PMID: 30087351
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

P Periasamy et al. (2018), Identification of genes which regulate stroma-dependent in vitro hematopoiesis PLoS ONE, 13(10):e0205583.
PMID: 30308055
J Heliste et al. (2018), Receptor tyrosine kinase profiling of ischemic heart identifies ROR1 as a potential therapeutic target BMC Cardiovasc Disord, 18(1):196.
PMID: 30342492
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

BA Brown et al. (2018), Aging differentially modulates the Wnt pro-survival signalling pathways in vascular smooth muscle cells Aging Cell, 0(0):e12844.
PMID: 30548452
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Ogorodniko et al. (2018), Transcriptome 3'end organization by PCF11 links alternative polyadenylation to formation and neuronal differentiation of neuroblastoma Nat Commun, 9(1):5331.
PMID: 30552333
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Baio et al. (2018), Spaceflight Activates Protein Kinase C Alpha Signaling and Modifies the Developmental Stage of Human Neonatal Cardiovascular Progenitor Cells Stem Cells Dev., 0(0).
PMID: 29320953
Species: Human
Sample Types: Whole Cells
Applications: Differentiation

DE Reichman et al. (2018), Wnt inhibition promotes vascular specification of embryonic cardiac progenitors Development, 145(1).
PMID: 29217753
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

I Ackers et al. (2018), Blocking Wnt5a signaling decreases CD36 expression and foam cell formation in atherosclerosis Cardiovasc. Pathol., 34(0):1-8.
PMID: 29474941
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Rosengren et al. (2018), TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms Cell. Mol. Life Sci., 0(0).
PMID: 29396625
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Z Li et al. (2018), Wnt5a suppresses inflammation-driven intervertebral disc degeneration via a TNF-Î±/NF-ÎºB -Wnt5a negative-feedback loop Osteoarthr. Cartil., 0(0).
PMID: 29656141
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

YK Cho et al. (2018), Effect of SFRP5 (Secreted Frizzled-Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With Arterial Stiffness in Human Subjects Arterioscler. Thromb. Vasc. Biol., 0(0).
PMID: 29674475
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MK Hasan et al. (2018), Wnt5a induces ROR1 to recruit DOCK2 to activate Rac1/2 in chronic lymphocytic leukemia Blood, 0(0).
PMID: 29678828
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Kessenbroc et al. (2017), Diverse regulation of mammary epithelial growth and branching morphogenesis through noncanonical Wnt signaling Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28270600
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Skaria et al. (2017), WIF1 prevents Wnt5A mediated LIMK/CFL phosphorylation and adherens junction disruption in human vascular endothelial cells J Inflamm (Lond), 14(0):10.
PMID: 28529460
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

O Bernatik et al. (2017), A Novel Role for the BMP Antagonist Noggin in Sensitizing Cells to Non-canonical Wnt-5a/Ror2/Disheveled Pathway Activation Front Cell Dev Biol, 5(0):47.
PMID: 28523267
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Jang et al. (2017), WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells Biochem. Biophys. Res. Commun, 0(0).
PMID: 28137581
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Skaria et al. (2017), Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells Cell Adh Migr, 11(1):24-38.
PMID: 27159116
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

MW Susman et al. (2017), Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the control of cell and tissue behaviors in vertebrates Elife, 6(0).
PMID: 28885975
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KB Schou et al. (2017), KIF13B establishes a CAV1-enriched microdomain at the ciliary transition zone to promote Sonic hedgehog signalling Nat Commun, 8(0):14177.
PMID: 28134340
Species: Human
Sample Types: Whole Cells

P Paclíková et al. (2017), N-terminal part of Dishevelled DEP domain is required for Wnt/?-catenin signaling in mammalian cells Mol. Cell. Biol., 0(0).
PMID: 28674183
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Chen et al. (2017), Generation of iPSC-derived limb progenitor-like cells for stimulating phalange regeneration in the adult mouse Cell Discov, 3(0):17046.
PMID: 29263795
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R Behera et al. (2017), Inhibition of age-related therapy resistance in melanoma by rosiglitazone-mediated induction of Klotho Clin. Cancer Res, 0(0).
PMID: 28232477
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Strakova et al. (2017), The tyrosine Y250(2.39) in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled Cell. Signal., 38(0):85-96.
PMID: 28668722
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

MMF Steinbusch et al. (2017), Expression of RMRP RNA is regulated in chondrocyte hypertrophy and determines chondrogenic differentiation Sci Rep, 7(1):6440.
PMID: 28743979
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Z Prgomet et al. (2017), Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance Eur. J. Oral Sci., 0(0).
PMID: 28603941
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

I Savvidou et al. (2017), Beta-catenin inhibitor BC2059 is efficacious as monotherapy or in combination with proteasome inhibitor bortezomib in multiple myeloma Mol. Cancer Ther., 0(0).
PMID: 28500235
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Ndoye et al. (2017), ATG5 mediates a positive feedback loop between Wnt signaling and autophagy in melanoma Cancer Res., 0(0).
PMID: 28887323
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Gao et al. (2017), Ring finger protein 43 associates with gastric cancer progression and attenuates the stemness of gastric cancer stem-like cells via the Wnt-?/catenin signaling pathway Stem Cell Res Ther, 8(1):98.
PMID: 28446252
Species: Mouse
Sample Types: Whole Embryo
Applications: Bioassay

K Minegishi et al. (2017), A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking Dev. Cell, 40(5):439-452.e4.
PMID: 28292423
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Poobalasin et al. (2017), Heterozygous Vangl2(Looptail) mice reveal novel roles for the planar cell polarity pathway in adult lung homeostasis and repair Dis Model Mech, 0(0).
PMID: 28237967
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Jang et al. (2017), LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974 Sci Rep, 7(0):41612.
PMID: 28128299
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Vesel et al. (2017), ABCB1 and ABCG2 drug transporters are differentially expressed in non-small cell lung cancers (NSCLC) and expression is modified by cisplatin treatment via altered Wnt signaling Respir. Res, 18(1):52.
PMID: 28340578
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Petersen et al. (2017), Agonist-induced dimer dissociation as a macromolecular step in G protein-coupled receptor signaling Nat Commun, 8(1):226.
PMID: 28790300
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MK Connacher et al. (2017), Rear-polarized Wnt5a-receptor-actin-myosin-polarity (WRAMP) structures promote the speed and persistence of directional cell migration Mol. Biol. Cell, 0(0).
PMID: 28592632
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hoeke A Baarsma (2016), Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD J. Exp. Med, 0(0).
PMID: 27979969
Species: N/A

Applications: Bioassay

(2016), Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation J Biol Chem, 0(0).
PMID: 27402827
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling PLoS ONE, 11(5):e0156002.
PMID: 27214384
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ronald A DePinho (2016), Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth Cell, 167(5):1281-1295.e18.
PMID: 27863244
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

O Lyros et al. (2016), Dysregulation of WNT5A/ROR2 Signaling Characterizes the Progression of Barrett's-associated Esophageal Adenocarcinoma Mol Cancer Res, 0(0).
PMID: 27084312
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tu Z et al. (2016), Uterine RAC1 via Pak1-ERM signaling directs normal luminal epithelial integrity conducive to on-time embryo implantation in mice. Cell Death Differ, 23(1):169-81.
PMID: 26184908
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), KLF4 transcriptionally activates non-canonical WNT5A to control epithelial stratification Sci Rep, 6(0):26130.
PMID: 27184424
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tiwary S et al. (2016), FRIZZLED7 Is Required for Tumor Inititation and Metastatic Growth of Melanoma Cells. PLoS ONE, 11(1):e0147638.
PMID: 26808375
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms Nat. Med, 0(0).
PMID: 27918563
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Horigane S et al. (2016), Facilitation of axon outgrowth via a Wnt5a-CaMKK-CaMKIalpha pathway during neuronal polarization. Mol Brain, 9(1):8.
PMID: 26772170
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Identification of proliferative and mature ?-cells in the islets of Langerhans Nature, 535(7612):430-4.
PMID: 27398620
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), WNT5A signaling impairs breast cancer cell migration and invasion via mechanisms independent of the epithelial-mesenchymal transition J Exp Clin Cancer Res, 35(1):144.
PMID: 27623766
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

(2016), Wnt5a induces renal AQP2 expression by activating calcineurin signalling pathway Nat Commun, 7(0):13636.
PMID: 27892464
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Increased Wnt5a in squamous cell lung carcinoma inhibits endothelial cell motility BMC Cancer, 16(1):915.
PMID: 27876017
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Omar Farah (2016), Porcupine-dependent Wnt signaling controls stromal proliferation and endometrial gland maintenance through the action of distinct WNTs Dev. Biol, 0(0).
PMID: 27965056
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Regeneration of Articular Cartilage by Human ESC-Derived Mesenchymal Progenitors Treated Sequentially With BMP-2 and Wnt5a Stem Cells Transl Med, 0(0).
PMID: 27496569
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ouji Y et al. (2015), Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro. J Invest Dermatol, 135(6):1598-608.
PMID: 25437427
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Suryawanshi A et al. (2015), Canonical wnt signaling in dendritic cells regulates Th1/Th17 responses and suppresses autoimmune neuroinflammation. J Immunol, 194(7):3295-304.
PMID: 25710911
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Zhao D et al. (2015), WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features. BMC Gastroenterol, 15(0):173.
PMID: 26652024
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Handorf A et al. (2015), Endogenously produced Indian Hedgehog regulates TGFbeta-driven chondrogenesis of human bone marrow stromal/stem cells. Stem Cells Dev, 24(8):995-1007.
PMID: 25519748
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lee J et al. (2014), Interleukin-1beta-induced Wnt5a enhances human corneal endothelial cell migration through regulation of Cdc42 and RhoA. Mol Cell Biol, 34(18):3535-45.
PMID: 25022753
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tsai H et al. (2014), Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways. PLoS ONE, 9(8):e104937.
PMID: 25170755
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Liu et al. (2014), Wnt5a through noncanonical Wnt/JNK or Wnt/PKC signaling contributes to the differentiation of mesenchymal stem cells into type II alveolar epithelial cells in vitro. PLoS ONE, 9(3):e90229.
PMID: 24658098
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Yu C et al. (2014), Recombinant Wnt3a and Wnt5a elicit macrophage cytokine production and tolerization to microbial stimulation via Toll-like receptor 4. Eur J Immunol, 44(5):1480-90.
PMID: 24643512
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ekstrom E et al. (2014), WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells. Mol Cancer, 13(0):88.
PMID: 24766647
Species: Rat
Sample Types: Tissue Homogenates
Applications: Bioassay

Dawes L et al. (2014), Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism. Dev Biol, 385(2):291-303.
PMID: 24211762
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhang A et al. (2014), Wnt5a promotes migration of human osteosarcoma cells by triggering a phosphatidylinositol-3 kinase/Akt signals. Cancer Cell Int, 14(1):15.
PMID: 24524196
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Povinelli B et al. (2014), Wnt5a regulates hematopoietic stem cell proliferation and repopulation through the Ryk receptor. Stem Cells, 32(1):105-15.
PMID: 23939973
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Many A et al. (2014), Both canonical and non-canonical Wnt signaling independently promote stem cell growth in mammospheres. PLoS ONE, 9(7):e101800.
PMID: 25019931
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kilander M et al. (2014), Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 26(9):1943-9.
PMID: 24873871
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Godoy J et al. (2014), Wnt-5a ligand modulates mitochondrial fission-fusion in rat hippocampal neurons. J Biol Chem, 289(52):36179-93.
PMID: 25336659
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Bainbridge T et al. (2014), Evolutionary divergence in the catalytic activity of the CAM-1, ROR1 and ROR2 kinase domains. PLoS ONE, 9(7):e102695.
PMID: 25029443
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Smith A et al. (2014), Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart. Nat Protoc, 9(7):1662-81.
PMID: 24945383
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Keats E et al. (2014), Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis. Stem Cells, 32(6):1649-60.
PMID: 24496952
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhao C et al. (2014), GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation. PLoS ONE, 9(1):e85058.
PMID: 24416340
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Okamoto M et al. (2014), Noncanonical Wnt5a enhances Wnt/beta-catenin signaling during osteoblastogenesis. Sci Rep, 4(0):4493.
PMID: 24670389
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zhao Y et al. (2014), Wnt5a promotes inflammatory responses via nuclear factor kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) pathways in human dental pulp cells. J Biol Chem, 289(30):21028-39.
PMID: 24891513
Species: N/A
Sample Types: N/A
Applications: Western Blot

Ishizuya-Oka et al. (2014), Thyroid hormone-regulated Wnt5a/Ror2 signaling is essential for dedifferentiation of larval epithelial cells into adult stem cells in the Xenopus laevis intestine. PLoS ONE, 9(9):e107611.
PMID: 25211363
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Linnskog R et al. (2014), Interleukin-6 drives melanoma cell motility through p38alpha-MAPK-dependent up-regulation of WNT5A expression. Mol Oncol, 8(8):1365-78.
PMID: 24954857
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Bentzinger C et al. (2014), Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. J Cell Biol, 205(1):97-111.
PMID: 24711502
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lee G et al. (2014), Prostate cancer bone metastases acquire resistance to androgen deprivation via WNT5A-mediated BMP-6 induction. Br J Cancer, 110(6):1634-44.
PMID: 24518599
Li S et al. (2014), IL-1beta mediates MCP-1 induction by Wnt5a in gastric cancer cells. BMC Cancer, 14(0):480.
PMID: 24993819
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Cajanek L et al. (2013), Tiam1 regulates the Wnt/Dvl/Rac1 signaling pathway and the differentiation of midbrain dopaminergic neurons. Mol Cell Biol, 33(1):59-70.
PMID: 23109420
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Thrasivoulou C et al. (2013), Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 288(50):35651-9.
PMID: 24158438
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Li B et al. (2013), Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways. J Biol Chem, 288(19):13610-9.
PMID: 23539626
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Florian M et al. (2013), A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing. Nature, 503(7476):392-6.
PMID: 24141946
Ye Z et al. (2013), Wnt5a uses CD146 as a receptor to regulate cell motility and convergent extension. Nat Commun, 4(0):2803.
PMID: 24335906
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Goel S et al. (2012), Both LRP5 and LRP6 receptors are required to respond to physiological Wnt ligands in mammary epithelial cells and fibroblasts. J. Biol. Chem., 287(20):16454-66.
PMID: 22433869
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tran H et al. (2012), Reversible modification of adenomatous polyposis coli (APC) with K63-linked polyubiquitin regulates the assembly and activity of the beta-catenin destruction complex. J Biol Chem, 287(34):28552-63.
PMID: 22761442
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Cohen E et al. (2012), Wnt5a and Wnt11 are essential for second heart field progenitor development. Development, 139(11):1931-40.
PMID: 22569553
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Blakely BD et al. (2011), Wnt5a regulates midbrain dopaminergic axon growth and guidance. PLoS ONE, 6(3):e18373.
PMID: 21483795
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Scheel C et al. (2011), Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell, 145(6):926-40.
PMID: 21663795
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kilander MB et al. (2011), WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins. Cell. Signal., 23(3):550-4.
PMID: 21070854
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Li B et al. (2011), WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity. PLoS ONE, 6(8):e22920.
PMID: 21857966
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Bourhis E et al. (2010), Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6. J. Biol. Chem., 285(12):9172-9.
PMID: 20093360
Species: Human
Sample Types: Whole Cells

Gudjonsson JE et al. (2010), Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 130(7):1849-59.
PMID: 20376066
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

O'Connell MP et al. (2010), The orphan tyrosine kinase receptor, ROR2, mediates Wnt5A signaling in metastatic melanoma. Oncogene, 29(1):34-44.
PMID: 19802008
Species: Zebrafish
Sample Types: Whole Cells
Applications: Bioassay

Ma LH et al. (2009), Establishment of a transitory dorsal-biased window of localized Ca2+ signaling in the superficial epithelium following the mid-blastula transition in zebrafish embryos. Dev. Biol., 327(1):143-57.
PMID: 19133253
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Verkaar F et al. (2009), Stably overexpressed human Frizzled-2 signals through the beta-catenin pathway and does not activate Ca2+-mobilization in Human Embryonic Kidney 293 cells. Cell. Signal., 21(1):22-33.
PMID: 18929644
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Steele BM et al. (2009), Canonical Wnt signaling negatively regulates platelet function. Proc. Natl. Acad. Sci. U.S.A., 106(47):19836-41.
PMID: 19901330
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Gogolla N et al. (2009), Wnt signaling mediates experience-related regulation of synapse numbers and mossy fiber connectivities in the adult hippocampus. Neuron, 62(4):510-25.
PMID: 19477153
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

O'Connell MP et al. (2009), Wnt5A activates the calpain-mediated cleavage of filamin A. J. Invest. Dermatol., 129(7):1782-9.
PMID: 19177143
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Endo Y et al. (2008), Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled3- and c-Jun N-terminal kinase-dependent mechanism. Mol. Cell. Biol., 28(7):2368-79.
PMID: 18212053
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Safholm A et al. (2008), The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility. Clin. Cancer Res., 14(20):6556-63.
PMID: 18927296
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Matushansky I et al. (2007), Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway. J. Clin. Invest., 117(11):3248-57.
PMID: 17948129
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Masckauchan TN et al. (2006), Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2. Mol. Biol. Cell, 17(12):5163-72.
PMID: 17035633
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Henry C et al. (0), Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion. Oncotarge6(37):40310-26.
PMID: 26515598
Species: Human
Sample Types: Organoid
Applications: Bioassay

Ideno H et al. (0), Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanisms. iScienc25(10):105140.
PMID: 36185382

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Recombinant Human/Mouse Wnt-5a Protein

Name: Anonymous

Application: CellProlif

Reason for Rating: wnt5a was used to test if it an promote stem cell proliferation.

Verified Customer | Posted 06/18/2022

put it in -80 freezer
Recombinant Human/Mouse Wnt-5a Protein

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 03/01/2022

Kept in -80 freezer
Recombinant Human/Mouse Wnt-5a Protein

Name: Anonymous

Application: Enzymatic activity in vitro

Verified Customer | Posted 11/29/2021

avoid freezing and refreezing, and kept in -80
Recombinant Human/Mouse Wnt-5a Protein

Name: Anonymous

Application: Luciferase activity.

Reason for Rating: very good product

Verified Customer | Posted 08/06/2021

Try to avoid thaw and freeze many times.
Recombinant Human/Mouse Wnt-5a Protein

Name: Anonymous

Application: Enzymatic activity in vitro

Reason for Rating: it worked very good.

Verified Customer | Posted 08/06/2021

preserve it under -20 after reconstitution
Recombinant Human/Mouse Wnt-5a Protein

Name: Gerre Lafond

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 02/19/2021
Recombinant Human/Mouse Wnt-5a Protein

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Verified Customer | Posted 10/07/2020
Recombinant Human/Mouse Wnt-5a Protein

Name: Jignesh Patel

Application: In vitro bioactivity in cell culture

Reason for Rating: Works great with invitro studies

Verified Customer | Posted 03/22/2018

Recombinant Human/Mouse Wnt-5a Protein

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Recombinant Human/Mouse Wnt-5a Protein

Recombinant Human/Mouse Wnt-5a Protein

Recombinant Human/Mouse Wnt-5a Protein

Recombinant Human/Mouse Wnt-5a Protein

Recombinant Human/Mouse Wnt-5a Protein

Recombinant Human/Mouse Wnt-5a Protein

Recombinant Human/Mouse Wnt-5a Protein

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