Recombinant Human MIF Protein Best Seller
Catalog # 289-MF | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Product Specifications
Source
Pro2-Ala115, with an N-terminal Met
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
Scientific Data Images for Recombinant Human MIF Protein
Recombinant Human MIF Protein SEC-MALS.
Recombinant Human MIF (Catalog # 289-MF) has a molecular weight (MW) of 32.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 289-MF
Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl with BSA as a carrier protein. |
Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 289-MF/CF
Formulation | Supplied as a 0.2 μm filtered solution in MES and NaCl. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: MIF
MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4 - 7). Secretion occurs nonclassically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha-helices and six beta-strands, four of which form a beta-sheet. The two remaining beta-strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50 - 65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49 - 65. On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine and rat MIF, respectively.
References
- Norand, E.F. and M. Leech (2005) Front. Biosci. 10:12.
- Donn, R.P. and D.W. Ray (2004) J. Endocrinol. 182:1.
- Calandra, T. and T. Roger (2003) Nat. Rev. Immunol. 3:791.
- Kozak, C.A. et al. (1995) Genomics 27:405.
- Weiser, W.Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86:7522.
- Paralkar, V. and G. Wistow (1994) Genomics 19:48.
- Wistow, G.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:1272.
- Flieger, O. et al. (2003) FEBS Lett. 551:78.
- Philo, J.S. et al. (2004) Biophys. Chem. 108:77.
- Sun, H-W. et al. (1996) Protein Eng. 9:631.
- Stamps, S.L. et. al. (2000) Biochemistry 39:9671.
- Nguyen, M.T. et al. (2003) J. Biol. Chem. 278:33654.
- Sato, A. et al. (2003) Dev. Comp. Immunol. 27:401.
- Bernhagen, J. et al. (1994) Biochemistry 33:14144.
- Leng, L. et al. (2003) J. Exp. Med. 197:1467.
- Meyer-Siegler, K.L. and P.L. Vera (2005) J. Urol. 173:615.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human MIF Protein
Product Specific Notices for Recombinant Human MIF Protein
For research use only
Citations for Recombinant Human MIF Protein (30)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human MIF Protein include:
-
Species: Human
Sample Types: Whole Cells
Applications: BioassayCS Rigazio et al. (2022-11-12), Involvement of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor in proinflammatory mechanisms promoting cardiovascular injury mechanisms promoting cardiovascular inflammation tThe combined action of glycoinositolphospholipid from Trypanosoma cruzi and macrophage migration inhibitory factor increases proinflammatory mediator production by cardiomyocytes and vascular endothelial cells Microbial pathogenesis, 2022-10(0):105881.
PMID: 36379373 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayHA Oh et al. (2020-05-28), Migration Inhibitory Factor in Conditioned Medium from Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells Stimulates Hair Growth Cells, 2020-09(6).
PMID: 32481584 -
Species: Human
Sample Types: Whole Cell
Applications: BioassayH Chen et al. (2020-01-21), LncRNA-NEAT1 from the competing endogenous RNA network promotes cardioprotective efficacy of mesenchymal stem cell-derived exosomes induced by macrophage migration inhibitory factor via the miR-142-3p/FOXO1 signaling pathway Stem Cell Res Ther, 2020-011(1):31.
PMID: 31964409 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayLA Hernández- et al. (2018-11-19), Functional MIF promoter haplotypes modulate Th17-related cytokine expression in peripheral blood mononuclear cells from control subjects and rheumatoid arthritis patients Cytokine, 2018-1115(0):89-96.
PMID: 30467094 -
Species: Human
Sample Types: Plasma
L Rijvers et al. (2018-09-25), The macrophage migration inhibitory factor pathway in human B cells is tightly controlled and dysregulated in multiple sclerosis Eur. J. Immunol., 2018-00(0).
PMID: 30160778 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayF Ietta et al. (2018-08-14), Role of the Macrophage Migration Inhibitory Factor (MIF) in the survival of first trimester human placenta under induced stress conditions Sci Rep, 2018-08(1):12150.
PMID: 30108299 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayJ Lang et al. (2018-07-05), An hPSC-Derived Tissue-Resident Macrophage Model Reveals Differential Responses of Macrophages to ZIKV and DENV Infection Stem Cell Reports, 2018-00(0).
PMID: 29983385 -
Applications: ELISA (Standard)JA Fraser et al. (2018-05-14), Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations Sci Rep, 2018-08(1):7506.
PMID: 29760395 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySU Noh et al. (2018-04-24), Expression of Macrophage Migration Inhibitory Factor in Footpad Skin Lesions with Diabetic Neuropathy Mol Pain, 2018-00(0):1744806918775.
PMID: 29690804 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayS Sugita et al. (2018-04-01), Natural Killer Cell Inhibition by HLA-E Molecules on Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells Invest. Ophthalmol. Vis. Sci., 2018-059(5):1719-1731.
PMID: 29610856 -
Species: Human,Llama
Sample Types: Recombinant Protein,In Vivo
Applications: Surface Plasmon Resonance (SPR,In VivoA Sparkes et al. (2018-01-25), Novel half-life extended anti-MIF nanobodies protect against endotoxic shock FASEB J., 2018-00(0):fj201701189R.
PMID: 29401625 -
Sample Types: Protein
Applications: BioassayR Jalili et al. (2018-01-16), Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies Proc. Natl. Acad. Sci. U.S.A., 2018-00(0).
PMID: 29339495 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayR Kumar et al. (2017-06-30), Modulating glioma-mediated myeloid-derived suppressor cell development with sulforaphane PLoS ONE, 2017-012(6):e0179012.
PMID: 28666020 -
Applications: ELISA (Standard)Neslihan Coban (2017-02-01), CYP19A1, MIF and ABCA1 genes are targets of the ROR? in monocyte and endothelial cells Cell Biol. Int, 2017-00(0).
PMID: 27925372 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayNo Y et al. (2015-09-09), HIF1alpha-Induced by Lysophosphatidic Acid Is Stabilized via Interaction with MIF and CSN5. PLoS ONE, 2015-010(9):e0137513.
PMID: 26352431 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySugita S et al. (2015-01-20), Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells. Invest Ophthalmol Vis Sci, 2015-056(2):1051-62.
PMID: 25604685 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayPatel P et al. (2014-02-13), The expression of HIV-1 Vpu in monocytes causes increased secretion of TGF-beta that activates profibrogenic genes in hepatic stellate cells. PLoS ONE, 2014-09(2):e88934.
PMID: 24551192 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayLi J et al. (2012-08-14), Tumor Microenvironment Macrophage Inhibitory Factor Directs the Accumulation of Interleukin-17-producing Tumor-infiltrating Lymphocytes and Predicts Favorable Survival in Nasopharyngeal Carcinoma Patients. J Biol Chem, 2012-0287(42):35484-95.
PMID: 22893706 -
Species: Mouse
Sample Types: In Vivo
Applications: In VivoHerrero LJ et al. (2011-07-05), Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis. Proc. Natl. Acad. Sci. U.S.A., 2011-0108(29):12048-53.
PMID: 21730129 -
Sample Types: N/A
Applications: ELISA (Standard)Schipper HS et al. (2010-06-08), A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-056(0):1320.
PMID: 20530731 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayHuang MC et al. (2010-02-12), Human CD4- 8- T cells are a distinctive immunoregulatory subset. FASEB J, 2010-024(7):2558-66.
PMID: 20154266 -
Species: Bovine
Sample Types: Whole Cells
Applications: BioassayWadgaonkar R et al. (2008-12-01), Thrombin induced secretion of macrophage migration inhibitory factor (MIF) and its effect on nuclear signaling in endothelium. J. Cell. Biochem., 2008-1105(5):1279-88.
PMID: 18821572 -
Species: Mouse
Sample Types: In Vivo
Applications: In VivoGilliver SC et al. (2008-07-24), Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factor. Endocrinology, 2008-0149(11):5747-57.
PMID: 18653719 -
Species: Human
Sample Types: N/A,Whole Cells
Applications: ELISA (Standard),BioassayAmin MA et al. (2005-11-29), Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB. Blood, 2005-1107(6):2252-61.
PMID: 16317091 -
Sample Types: N/A
Applications: ELISA (Standard)Matsumoto K et al. (2005-02-01), Elevated macrophage migration inhibitory factor (MIF) levels in the urine of patients with focal glomerular sclerosis. Clin. Exp. Immunol., 2005-0139(2):338-47.
PMID: 15654833 -
Sample Types: N/A
Applications: Western BlotPaulesu L et al. (2004-11-24), Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation. Biol. Reprod., 2004-172(4):949-53.
PMID: 15564603 -
Applications: ELISA (Standard)Chuang CC et al. (2004-03-01), High concentrations of circulating macrophage migration inhibitory factor in patients with severe blunt trauma: Is serum macrophage migration inhibitory factor concentration a valuable prognostic factor? Crit. Care Med., 2004-032(3):734-9.
PMID: 15090955 -
Species: Mouse
Sample Types: In Vivo
Applications: In VivoAshcroft GS et al. (2003-05-01), Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor. J. Clin. Invest., 2003-0111(9):1309-18.
PMID: 12727922 -
Sample Types: N/A
Applications: ELISA (Standard)Matsumoto K et al. (2002-10-01), Urinary levels of macrophage migration inhibitory factor in patients with IgA nephropathy. 7587, 2002-192(2):309-15.
PMID: 12218308 -
Sample Types: N/A
Applications: ELISA (Standard)Wang L et al. (2002-05-15), Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production. J. Immunol., 2002-0168(10):4889-96.
PMID: 11994438
There are no citations that match your criteria.
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.