Recombinant Human Mesothelin (aa 296-580) Fc Protein, CFNew

Name: Recombinant Human Mesothelin (aa 296-580) Fc Protein, CF
Brand: R&D Systems
SKU: 10959-MS

Catalog # 10959-MS

Product Datasheet / COA / SDS

C-Terminal

Order Information

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Key Product Details

Source:	HEK293
Accession #:	AAH09272.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Mesothelin (aa 296-580) Fc Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Mesothelin protein

Human Mesothelin (Glu296-Gly580) Accession # AAH09272.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu296

Predicted Molecular Mass

59 kDa

SDS-PAGE

65-80 kDa, reducing conditions.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CA125/MUC16 (Catalog # 5609-MU) is immobilized at 200 ng/mL (100 µL/well), Recombinant Human Mesothelin (aa 296-580) Fc Chimera binds with an ED₅₀ of 1.00-6.00 ng/mL.

Recombinant Human Mesothelin (aa 296-580) Fc Protein, CF Scientific Data Examples

	Recombinant Human Mesothelin C-Terminal (aa 296‑580) Fc Chimera Protein Binding Activity. When Recombinant Human CA125/MUC16 (5609-MU) is immobilized at 200 ng/mL (100 µL/well), Recombinant Human Mesothelin (aa 296-580) Fc Chimera Protein (Catalog # 10959-MS) binds with an ED₅₀ of 1.00-6.00 ng/mL.
	Recombinant Human Mesothelin C-Terminal (aa 296‑580) Fc Chimera Protein SDS-PAGE. 2 μg/lane of Recombinant Human Mesothelin C-Terminal (aa 296‑580) Fc Chimera Protein (Catalog # 10959-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-80 kDa and 130-160 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10959-MS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1.00 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: Mesothelin

Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and over-expressed in several human tumors (1, 2). The mesothelin gene encodes a ~70 kDa precursor protein that is cleaved at a dibasic proteolytic site into a 40 kDa membrane-bound protein termed MSLN and a 31 kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell (3, 4). Cleaved, human MSLN remains attached to the cell surface via a GPI linkage and shares 58% amino acid sequence identity with mouse and rat MSLN. In human, alternate splicing generates additional MSLN isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor (3). Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 4). It is up‑regulated in mesotheliomas and a range of carcinomas and adenomas (5-7). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression (7, 8). It is co‑expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (9). A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions (9-11).

Item	Value
References	Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136. Muminova, Z.E. et al. (2004) BMC Cancer 4:19. Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531. Ordonez, N.G. (2003) Mod. Pathol. 16:192. Argani, P. et al. (2001) Clin. Cancer Res. 7:3862. Li, M. et al. (2008) Mol. Cancer Ther. 7:286. Uehara, N. et al. (2008) Mol. Cancer Res. 6:186. Rump, A. et al. (2004) J. Biol. Chem. 279:9190. Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751. Robinson, B.W.S. et al. (2003) Lancet 362:1612.
Entrez Gene IDs	10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)
Alternate Names	CAK1, CAK1 antigen, MPF, MPFSMRP, MSLN, Mesothelin, Pre-pro-megakaryocyte-potentiating factor, SMR, megakaryocyte potentiating factor, soluble MPF mesothelin related protein

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