Recombinant Human CA125/MUC16 Protein, CF

Name: Recombinant Human CA125/MUC16 Protein, CF
Brand: R&D Systems
SKU: 5609-MU

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 5609-MU

Citations (11)

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Source:	CHO
Accession #:	NP_078966.2
Applications:	Binding Activity

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Product Specifications for Recombinant Human CA125/MUC16 Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human CA125/MUC16 protein Met13360-Gln14347 (Met13472Thr & Gln13957Lys) with a C-terminal 6-His tag
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met13360
Predicted Molecular Mass	110 kDa
SDS-PAGE	170-200 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human CA125/MUC16 is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin‑3 (Catalog # 1154-GA) that produces 50% of the optimal binding response is found to be approximately 0.35-1.4 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5609-MU

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CA125/MUC16

MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy (1, 2). Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human MUC16 that can vary from 1148 to 22152 amino acids (aa) in length and 200 - 5000 kDa in size (1, 2). The 22152 aa form contains ser/thr-rich N-terminal tandem repeats, 4 LRR (Leu-rich repeat) domains, 56 SEA (sea urchin sperm protein, enterokinase and agrin) domains, a transmembrane domain, and a 31 aa cytoplasmic domain that includes a tyrosine phosphorylation site (1-4). SEA domains are ~120 aa in length, contain conserved residues including potential O-glycosylation sites and a pair of cysteines, and are often found in transmembrane mucins (3). The protein produced by R&D Systems represents aa 13360-14347 of the full sequence and includes the last 6 SEA domains. It shares 68% aa identity with canine MUC16. MUC16 is over-expressed by tumor cells including ovarian and mesothelial cancers (5). The transmembrane form can adhere to mesothelin in the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity (5-7). MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells (8). MUC16-expressing tumors adhere to NK cells, down-regulate CD16 and suppress NK response, which may promote immune evasion (9, 10). MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy (10). In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome (11).

Item	Value
References	Yin, B. W. T. K. O. Lloyd, 2001, J. Biol. Chem. 276:27371. Maeda, T. et al. (2004) J. Biol. Chem. 279:13174. Fendrick, J. L. et al. (1997) Tumour Biol. 18:278. Swissprot accession Q8WXI7. Kaneko, O. et al. (2009) J. Biol. Chem. 284:3739. Rump, A. et al. (2004) J. Biol. Chem. 279:9190. Gubbels, J. A. A. et al. (2006) Mol. Cancer 5:50. Seelenmeyer, C. et al. (2003) J. Cell Sci. 116:1305. Patankar, M. S. et al. (2005) Gynecol. Oncol. 99:704. Belisle, J. A. et al. (2007) Immunology 122:418. Blalock, T. D. et al. (2007) Invest. Ophthalmol. Vis. Sci. 48:4509.
Long Name	Ovarian Cancer Antigen CA125
Entrez Gene IDs	94025 (Human)
Alternate Names	CA-125, CA125, CA125 ovarian cancer antigen, CA125MUC-16, FLJ14303, MUC16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, mucin 16, cell surface associated, mucin-16

Citations for Recombinant Human CA125/MUC16 Protein, CF (11)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human CA125/MUC16 Protein, CF include:

Species: Akkermansia muciniphila
Sample Types: Protein
Applications: Bioassay

DJ Shon et al. (2022), Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences The Journal of Biological Chemistry, 0(0):101917.
PMID: 35405095
Species: Human
Sample Types: Recombinant Proteins
Applications: Bioassay

LM Molinos-Al et al. (2022), Transient viral exposure drives functionally-coordinated humoral immune responses in HIV-1 post-treatment controllers Nature Communications, 13(1):1944.
PMID: 35410989
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

H Shaban et al. (2021), Label-Free Detection and Spectrometrically Quantitative Analysis of the Cancer Biomarker CA125 Based on Lyotropic Chromonic Liquid Crystal Biosensors, 11(8).
PMID: 34436073
Species: N/A
Sample Types: Film
Applications: ELISA

PC Wu et al. (2021), A Single-Substrate Biosensor with Spin-Coated Liquid Crystal Film for Simple, Sensitive and Label-Free Protein Detection Biosensors, 11(10).
PMID: 34677330
Species: Human
Sample Types: Protein
Applications: Reference Standard

J Song et al. (2021), Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays Biomedicines, 9(12).
PMID: 34944713
Species: N/A
Sample Types: Recombinant Protein
Applications: Binding Assay

KA Malsagova et al. (2020), Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor Biosensors, 10(12).
PMID: 33353197
Species: Bacteria - E. Coli
Sample Types: Recombinant Protein
Applications: Bioassay

SA Malaker et al. (2019), The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 30910957
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay

JK Fierle et al. (2019), Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery Sci Rep, 9(1):12815.
PMID: 31492910
Species: Mouse
Sample Types: Plasma
Applications: ELISA (Standard)

(2016), Establishment of patient-derived tumor xenograft models of epithelial ovarian cancer for pre-clinical evaluation of novel therapeutics Clin Cancer Res, 0(0).
PMID: 27573169
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Fischer N et al. (2015), Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG. Nat Commun, 6(0):6113.
PMID: 25672245
Species: N/A
Sample Types: Recombinant Protein
Applications: Western Blot

Prassas et al. (2014), False biomarker discovery due to reactivity of a commercial ELISA for CUZD1 with cancer antigen CA125. Clin Chem, 60(2):381-8.
PMID: 24097894

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Recombinant Human CA125/MUC16 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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