Recombinant Human Meprin alpha Subunit/MEP1A Protein, CF

Name: Recombinant Human Meprin alpha Subunit/MEP1A Protein, CF
Brand: R&D Systems
SKU: 3220-ZN

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3220-ZN

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	AAA21338
Structure / Form:	Disulfide-linked homodimer
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human Meprin alpha Subunit/MEP1A Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Meprin alpha Subunit/MEP1A protein Val22-Gln601 & Leu27-Gln601, both with a C-terminal 10-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val22 and Leu27
Predicted Molecular Mass	67 kDa
SDS-PAGE	86 kDa, reducing conditions
Activity	Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007). The specific activity is >500 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3220-ZN

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: Meprin alpha Subunit/MEP1A

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide-linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 746 amino acid sequence of human meprin alpha subunit precursor consists of a signal peptide (residues 1 to 21), a pro region (residues 22 to 65), and a mature chain (residues 66 to 746) containing following domains, catalytic (residues 62 to 263), MAM (residues 264 to 433), MATH (residues 434 to 593), EGF-like (residues 670 to 710), transmembrane (residues 713 to 740), and cytoplasmic (residues 741 to 746). The pro enzyme terminating at residue 601 was expressed and the secreted protein purified from conditioned medium. The molecular masses of recombinant human MEP1A are similar to those observed for the alpha subunit of rat Meprin A (5).

Item	Value
References	Bond, J.S. and Beynon, R.J. (1995) Protein Sci. 4:1247. Stocker, W. et al. (1995) Protein Sci. 4:823. Bertenshaw, G.P., et al. (2001) J. Biol. Chem. 276:13248. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895. Bertenshaw, G.P., et al. (2003) J. Biol. Chem. 278:2522.
Entrez Gene IDs	4224 (Human); 17287 (Mouse); 25684 (Rat)
Alternate Names	EC 3.4.24, EC 3.4.24.18, MEP1A, Meprin alpha Subunit, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, PABA peptide hydrolase, PPH alpha, PPHA, bA268F1.1 (meprin A alpha (PABA peptide hydrolase)), endopeptidase-2, meprin A subunit alpha, meprin A, alpha (PABA peptide hydrolase)

Citations for Recombinant Human Meprin alpha Subunit/MEP1A Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Meprin alpha Subunit/MEP1A Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

V Biasin et al. (2017), Meprin ? contributes to collagen deposition in lung fibrosis Sci Rep, 7(0):39969.
PMID: 28059112
Species: N/A
Sample Types: N/A
Applications: Zymography

Van Hiel et al. (2012), The ovicidal, larvacidal and adulticidal properties of 5,5'-dimethyl-2,2'-bipyridyl against Drosophila melanogaster. PLoS ONE, 7(11):e49961.
PMID: 23185497
Species: Human

Applications: Bioassay

Tsuchiya S et al. (2010), Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp). J. Bone Miner. Res., 26(0):220.
PMID: 20687161

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Recombinant Human Meprin alpha Subunit/MEP1A Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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