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Recombinant Human Ly6K Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10648-L6

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10648-L6-050

Key Product Details

Source

CHO

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Ly6K protein
Human Ly6K
(Asp18-Gly138)
Accession # Q17RY6.2
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp18

Predicted Molecular Mass

41 kDa

SDS-PAGE

41-61 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 0.75-7.5 μg/mL.

Scientific Data Images for Recombinant Human Ly6K Fc Chimera Protein, CF

Recombinant Human Ly6K Fc Chimera Protein Bioactivity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.75-7.5 μg/mL.

Recombinant Human Ly6K Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human Ly6K Fc Chimera Protein (Catalog # 10648-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-61 kDa and 82-122 kDa, respectively.

Formulation, Preparation and Storage

10648-L6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly6K

Lymphocyte antigen 6 complex, locus K (Ly6K) is a member of the LY6/urokinase-type plasminogen activator receptors (uPARS) family which participate in a wide range of functions from cell proliferation and migration to cytokine production (1). In humans, there are over 30 Ly6/uPARS members, characterized by a conserved, three-fingered disulfide linked LU domain that creates structural motif and a glycosylphosphatidylinositol (GPI)-anchoring site (2, 3). Members of the family are categorized as transmembrane or secretory depending on the presence of a GPI-anchored signal sequence. Mature LY6K contains an extracellular domain (ECD) with a single LU domain and a GPI-anchoring site. Within the ECD, human Ly6K shares 39% and 36% amino acid sequence identity with mouse and rat Ly6K, respectively. Human Ly6K is strongly expressed in testis and is crucial for the production of fertile spermatozoa (4). Additionally, Ly6K has been shown to be upregulated in a number of types of cancer and promotes tumor cell proliferation and metastasis and is a potential cancer therapy target (5). Furthermore, Ly6K promotes glioblastoma tumorigenicity via caveolin-1–mediated ERK1/2 signaling (6). The over-expression of Ly6K suppresses T cell development in the in vivo mouse model (7).

References

  1. Loughner, C.L. et al. (2016) Hum Genomics. 10:10.
  2. Upadhyay, G. (2019) Front. Immunol. 10:819.
  3. Song, D. et al. (2018) Oncol. Lett. 17:379.
  4. Endo S. et al. (2016) Sci. Rep. 6:23616.
  5. Benti, S. et al. (2020) Cancers 12:509.
  6. Sastry, N.G. et al. (2020) Neuro-Oncology. 22:1315.
  7. Son D. et al. (2019) Oncol. Lett. 17:379.

Long Name

Lymphocyte Antigen 6 Complex Locus K

Alternate Names

CO16, CT97

Entrez Gene IDs

54742 (Human); 76486 (Mouse)

Gene Symbol

LY6K

UniProt

Additional Ly6K Products

Product Documents for Recombinant Human Ly6K Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Ly6K Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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