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Recombinant Human Ly6D Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 10169-L6

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10169-L6-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Ly6D protein
Human Ly6D
(Leu21-Asn98)
Accession # Q14210
GGGS
Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu21

Predicted Molecular Mass

35 kDa

SDS-PAGE

38-46 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 1-7.5 μg/mL.

Scientific Data Images for Recombinant Human Ly6D Fc Chimera Protein, CF

Recombinant Human Ly6D Fc Chimera Protein Bioactivity

Recombinant Human Ly6D Fc Chimera Protein Bioactivity

Recombinant Human Ly6D Fc Chimera (Catalog # 10169-L6) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 1-7.5 μg/mL.
Recombinant Human Ly6D Fc Chimera Protein SDS-PAGE

Recombinant Human Ly6D Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human Ly6D Fc Chimera (Catlog # 10169-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 38-46 kDa and 80-90 kDa, respectively.

Formulation, Preparation and Storage

10169-L6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly6D

Ly6D (Lymphocyte antigen 6D), also known as E48 antigen, is a member of the lymphostromal cell membrane Ly6 protein superfamily (1, 2). There are at least twenty different human Ly6 proteins that have been identified, ranging from 11 to 36 kDa. These are classified as either secreted, or membrane-bound proteins which have the GPI-anchor signaling sequence. Included in the GPI-anchored cell surface glycoproteins are Ly6A-I and Ly6K (2-4). Human Ly6D is synthesized as a 128 amino acid (aa) protein that includes a 20 aa signal peptide, a 78 aa Ly6D chain, and a 30 aa propeptide. Within the main chain region, human Ly6D shares 64% and 58% aa sequence identity with mouse and rat Ly6D, respectively. Ly6D expression is significantly increased in bladder, brain and CNS, breast, head and neck, gastric, lung, ovarian, pancreatic, colorectal and kidney cancer than their counterpart normal tissues. High Ly6D expression is correlated with poor clinical outcome in brain and CNS, pancreatic, and colorectal, breast, colorectal, lung, gastric and ovarian cancer (4). LY6D is a progenitor marker that liaises intrinsically castration-resistant luminal cells and castration-resistant prostate tumor growth (5). LY6D is associated with an aggressive phenotype of ER-positive breast carcinoma, and these are potent markers for distant metastasis of ER-positive breast cancer patients (6).

References

  1. Gumley, T.P. et al. (1995) Immunol. Cell Biol. 73:277.
  2. Lv, Y. et al. (2018) Cell. Physiol. Biochem. 45:1219.
  3. Kong, H.K. and J.H. Park. (2012) BMB Rep. 45:595.
  4. Luo, L. et al. (2016) Oncotarget 7:11165.
  5. Barros-Silva JD, et al. (2018) Cell Rep. 25:3504
  6. Mayama A et al. (2018) Cancer Sci. 109:3350.

Long Name

Lymphocyte Antigen 6 Family Member D

Alternate Names

E48 Antigen, Ly-6D

Entrez Gene IDs

8581 (Human); 17068 (Mouse); 315075 (Rat)

Gene Symbol

LY6D

UniProt

Additional Ly6D Products

Product Documents for Recombinant Human Ly6D Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Ly6D Fc Chimera Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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