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Recombinant Human LSECtin/CLEC4G Protein, CF

Catalog # 2947-CL | R&D Systems, Inc. a Bio-Techne Brand
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2947-CL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LSECtin/CLEC4G protein
Ser54-Cys293, with an N-terminal 9-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser54

Predicted Molecular Mass

27.8 kDa

SDS-PAGE

37 kDa, reducing conditions

Activity

Measured by its ability to bind Jurkat human acute T cell leukemia cells in a flow cytometry assay.
A 2-8 fold increase in fluorescence intensity can be observed when Jurkat cells are stained with 10 µg/tube rhLSECtin/his and mouse anti-polyHistidine-PE as compared to cells stained with Mouse Anti-polyHistidine PE-conjugated Monoclonal Antibody (Catalog # IC050P).
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

2947-CL
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LSECtin/CLEC4G

LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), also known as C-type lectin superfamily 4, member G (CLEC4G), is a member of subgroup II of the C-type (Ca2+-dependent) lectin superfamily (1). The protein was named LSECtin because its initial expression was described to be restricted to liver and lymph node sinusoidal endothelial cells (1). Since then, however, LSECtin has also been detected in peripheral blood and thymic dendritic cells isolated ex vivo, and in monocyte-derived macrophages and dendritic cells at the RNA and protein level (2). Human LSECtin is an approximately 40 kDa, single-pass, type II transmembrane glycoprotein that is 293 amino acids (aa) in length. It contains a short N-terminal cytoplasmic tail (aa 1-31) and a 21 aa transmembrane region. Its extracellular region consists of two N-linked glycosylation sites (aa 73 and 159), a coil-coil neck domain (aa 96-136), a C-type lectin-like domain (CTLD) of the type found in human
DC-SIGN and DC-SIGN receptor (aa 165-289), and a C-terminal Ca2+-dependent carbohydrate-recognition domain (C-type CRD) (1). Human LSECtin shares 64% aa sequence identity with mouse LSECtin. LSECtin binds to mannose, GlcNAc, and fucose in a Ca2+-dependent manner (1-3). In addition, LSECtin has the ability to bind to surface glycoproteins of enveloped viruses (3, 4). In particular, interaction of LSECtin with the surface glycoproteins of severe acute respiratory syndrome (SARS) coronavirus and Ebola virus has been described, and LSECtin-mediated infection of cells by Ebola virus has been demonstrated (3, 4).

References

  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Dominguez-Soto, A. et al. (2007) Blood 109:5337.
  3. Powlesland, A. et al. (2008) J. Biol. Chem. 283:593.
  4. Gramberg, T. et al. (2005) Virology 340:224.

Long Name

Liver And Lymph Node Sinusoidal Endothelial Cell C-type Lectin

Alternate Names

CLEC4G, Q6UXB4

Entrez Gene IDs

339390 (Human); 75863 (Mouse)

Gene Symbol

CLEC4G

UniProt

Product Documents for Recombinant Human LSECtin/CLEC4G Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LSECtin/CLEC4G Protein, CF

For research use only

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