Key Product Details
Structure / Form
Accession # AAB68668
N-terminal Sequence Analysis
Predicted Molecular Mass
The ED50 for this effect is 0.4-2 µg/mL.
Scientific Data Images for Recombinant Human LILRB3/CD85a/ILT5 Fc Chimera Protein, CF
Recombinant Human LILRB3/CD85a/ILT5 Fc Chimera Protein BioactivityRecombinant Human LILRB3/CD85a/ILT5 Fc Chimera (Catalog # 1806-T5) binds fluorescein-conjugated S. aureus Bioparticles. The ED50 for this effect is 0.4-2 µg/mL.
Formulation, Preparation and Storage
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
Reconstitute at 250 μg/mL in PBS.
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
85-95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3). Alternative splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors (4-6). Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5, 7). On osteoclast precursors, LILRB3 ligation inhibits RANK L/TRANCE or M-CSF induced differentiation (8). LILRB3 can also bind to ligands exposed on necrotic tumor cells (9). Both PIR-B and LILRB3 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (3). R&D Systems in-house testing indicates that LILRB3 binds to
Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (10). In the mouse CNS, PIR-B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (11). Upon binding to MAG, PIR-B associates with TrkB and NGF R/p75 in cerebellar granule neurons (12).
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- Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
- Nakayama, M. et al. (2007) J. Immunol. 178:4250.
- Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1174.
- Sloane, D.E. et al. (2004) Blood 104:2832.
- Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
- Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
- Mori, Y. et al. (2008) J. Immunol. 181:4742.
- Jones, D.C. et al. (2016) Oncotarget PMID 26769854.
- Zheng, J. et al. (2012) Nature 485:656.
- Atwal, J.K. et al. (2008) Science 322:967.
- Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.
Entrez Gene IDs
Product Specific Notices for Recombinant Human LILRB3/CD85a/ILT5 Fc Chimera Protein, CF
For research use only