Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Name: Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF
Brand: R&D Systems
SKU: 8429-T4
Rating: 5 (2 reviews)

Catalog # 8429-T4 | R&D Systems, Inc. a Bio-Techne Brand

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Product Datasheet / COA / SDS

(HEK293-expressed)

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	Q8N423.4
Structure / Form:	Monomer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Product Specifications for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human LILRB2/CD85d/ILT4 protein Gln22-His458, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Thr23
Predicted Molecular Mass	48 kDa
SDS-PAGE	62-72 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human LILRB2/CD85d/ILT4 is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with a typical ED₅₀ of 25-150 ng/mL.

Scientific Data Examples for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

	Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein Bioactivity When Recombinant Human LILRB2/CD85d/ILT4 (Catalog # 8429-T4) is coated onto a microplate at 2 µg/mL, Recombinant Human Angiopoietin-like 7 Protein (Catalog # 914-AN) binds with a typical ED₅₀ of 25-150 ng/mL.
	Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein SDS-PAGE 1 μg/lane of Recombinant Human LILRB2/CD85d/ILT4 (Catalog # 8429-T4) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 69.9 and 66.8 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8429-T4

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

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Background: LILRB2/CD85d/ILT4

The immunoglobulin-like transcript (ILT) molecules comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR) (1, 2). ILT4, also known as CD85d, LIR-2, and LILRB2, is closely related to mouse PIR-B and is primarily expressed on monocytes, dendritic cells (DC), and neutrophils (3, 4). Mature human ILT4 consists of a 440 amino acid (aa) extracellular domain (ECD) with 4 Ig-like domains, a 21 aa transmembrane segment, and a 116 aa cytoplasmic domain with 3 inhibitory immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (5). The ECD of human ILT4 shares 76% aa identity with chimpanzee ILT4 and 74%, 81%, 33%, 52%, 77%, 61%, and 64% aa identity with human ILT1, 2, 3, 5, 6, 7, and 8, respectively. ILT4 binds to classical MHC I proteins as well as the non-classical HLA-G1 and HLA-F molecules (5-9). It competes with CD8 alpha for MHC I binding but does not compete with KIR2DL1 (7). Ligation of ILT4 induces tyrosine phosphorylation within its cytoplasmic ITIMs, association with SHP-1, and inhibition of stimulatory signaling (3, 6). ILT4 activation promotes the development of tolerogenic dendritic cells and the subsequent induction of regulatory T cells and CD4⁺ T cell anergy (10-12). Ligation of ILT4 on neutrophils further attenuates immune responses by inhibiting neutrophil phagocytic activity and production of reactive oxygen species (4). ILT4 also binds to multimeric Angiopoietin-like 2, and its ligation supports the expansion of hematopoietic stem cells from cord blood (13). In the brain, ILT4 and mouse PIR-B function as receptors for oligomeric Abeta (1-42) peptide (14).

Item	Value
References	Suciu-Foca, N. et al. (2005) Int. Immunopharmacol. 5:7. Hofmeister, V. and E.H. Weiss (2003) Semin. Canc. Biol. 13:317. Fanger, N.A. et al. (1998) Eur. J. Immunol. 28:3423. Baudhuin, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:17957. Borges, L. et al. (1997) J. Immunol. 159:5192. Colonna, M. et al. (1998) J. Immunol. 160:3096. Shiroishi, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:8856. Lepin, E.J.M. et al. (2000) Eur. J. Immunol. 30:3552. Allen, R.L. et al. (2001) J. Immunol. 167:5543. Chang, C.C. et al. (2002) Nat. Immunol. 3:237. Ristich, V. et al. (2005) Eur. J. Immunol. 35:1133. Gregori, S. et al. (2010) Blood 116:935. Deng, M. et al. (2014) Blood 124:924. Kim, T. et al. (2013) Science 341:1399.
Long Name	Leukocyte Immunoglobulin-like Receptor, Subfamily B (with TM and ITIM Domains), Member 5
Entrez Gene IDs	10288 (Human)
Alternate Names	CD85 antigen-like family member D, CD85d, CD85d antigen, ILT-4, ILT4, ILT4CD85d, Ig-like transcript 4, Immunoglobulin-like transcript 4, LILRB2, LIR-2subfamily A (with TM domain), member 6, LIR2, LIR2CD85D, Leukocyte immunoglobulin-like receptor 2, MIR10, MIR10LILRA6, Monocyte/macrophage immunoglobulin-like receptor 10, leukocyte immunoglobulin-like receptor subfamily B member 2, leukocyte immunoglobulin-like receptor subfamily B member 2 soluble isoform, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2

Citations for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

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Customer Reviews for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF (2)

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Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Name: Anonymous

Application: Binding assay/Protein-protein interaction

Reason for Rating: HLA-G reagent shows specific binding to ILT4. Binding is weak so could not get KD

Verified Customer | Posted 09/01/2021

Immobilize HLA-G and flow ILT4. Reverse orientation also shows binding
Name: Anonymous

Reason for Rating: Great reagent for capturing anti-RB2 antibody

Verified Customer | Posted 04/28/2020

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Product Documents for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

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Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Catalog # 8429-T4 | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Scientific Data Examples for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: LILRB2/CD85d/ILT4

Citations for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Customer Reviews for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF (2)

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Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Product Documents for Recombinant Human LILRB2/CD85d/ILT4 His-tag Protein, CF

Product Datasheets

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