Recombinant Human LIFR alpha (Mammalian-expressed), CF

Name: Recombinant Human LIFR alpha (Mammalian-expressed), CF
Brand: R&D Systems
SKU: 7487-LR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7487-LR

Citations (1)

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Key Product Details

Source:	HEK293
Accession #:	NP_001121143
Applications:	Bioactivity

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Product Specifications for Recombinant Human LIFR alpha (Mammalian-expressed), CF

Source	Human embryonic kidney cell, HEK293-derived human LIF R alpha protein Met1-Ser833
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gln45 predicted: No result obtained, sequencing might be blocked
Predicted Molecular Mass	89.4 kDa
SDS-PAGE	110-140 kDa, reducing conditions
Activity	Measured by its ability to inhibit LIF-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is typically 1-6 μg/mL in the presence of 0.3 ng/mL of recombinant human LIF.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7487-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 300 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature human LIF R alpha consists of a 789 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2). Within the ECD, human LIF R alpha shares 72% aa sequence identity with mouse and rat LIF R alpha. LIF R alpha binds the pleiotropic cytokine LIF with low affinity (3). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (4, 6). The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (4). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (6, 7). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (8, 9). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (10-12).

Item	Value
References	Muller-Newen, G. (2003) Science STKE 2003:pe40. Gearing, D.P. et al. (1991) EMBO J. 10:2839. Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616. Gearing, D.P. et al. (1992) Science 255:1434. Giese, B. et al. (2005) J. Cell Sci. 118:5129. Ip, N.Y. et al. (1992) Cell 69:1121. Davis, S. et al. (1993) Science 260:1805. Metcalf, D (2003) Stem Cells 21:5. Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319. Stewart, C.L. et al. (1992) Nature 359:76. Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.
Long Name	Leukemia Inhibitory Factor Receptor alpha
Entrez Gene IDs	3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)
Alternate Names	CD118, CD118 antigen, FLJ98106, FLJ99923, LIF R alpha, LIF R beta, LIF receptor, LIF-R, LIFR, LIFR alpha, LIFRa, SJS2, STWS, SWS, leukemia inhibitory factor receptor, leukemia inhibitory factor receptor alpha

Citations for Recombinant Human LIFR alpha (Mammalian-expressed), CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human LIFR alpha (Mammalian-expressed), CF include:

Species: Chicken
Sample Types: In Vivo
Applications: In Vivo

Salm F et al. (2015), The Phosphoinositide 3-Kinase p110alpha Isoform Regulates Leukemia Inhibitory Factor Receptor Expression via c-Myc and miR-125b to Promote Cell Proliferation in Medulloblastoma. PLoS ONE, 10(4):e0123958.
PMID: 25915540

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