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Recombinant Human LAIR2 Fc Chimera Protein, CF

Catalog # 10166-LR | R&D Systems, Inc. a Bio-Techne Brand
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10166-LR-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LAIR2 protein
Human LAIR2
(Gln22-Pro152)
Accession # Q6ISS4
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Glu23 inferred from enzymatic pyroglutamate treatment revealing Gln22

Predicted Molecular Mass

41 kDa

SDS-PAGE

50-56 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Human LAIR2 Fc Chimera binds with an ED50 of 0.04-0.32 ng/mL.

Scientific Data Images for Recombinant Human LAIR2 Fc Chimera Protein, CF

Recombinant Human LAIR2 Fc Chimera Protein Binding Activity

Recombinant Human LAIR2 Fc Chimera Protein Binding Activity

When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Human LAIR2 Fc Chimera (Catalog # 10166-LR) binds with an ED50 of 0.04-0.32 ng/mL.
Recombinant Human LAIR2 Fc Chimera Protein SDS-PAGE

Recombinant Human LAIR2 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human LAIR2 Fc Chimera (Catalog # 10166-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-56 kDa and 100-120 kDa, respectively.

Formulation, Preparation and Storage

10166-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR2

LAIR2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR1, its transmembrane counterpart, LAIR2 shares 83% aa identity across the signal sequence and extracellular domains (1-3). Although one is secreted and the other is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR2 may compete with LAIR1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C-terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported. LAIR2 is a soluble collagen-receptor, and it can be detected in the synovial fluid of rheumatoid arthritis patients, urine of pregnant women, and as well as primary cells (5, 6). In vitro studies have demonstrated LAIR2 can compete with LAIR1 for the same collagen binding site and suggesting LAIR2 may play an important role in immune cell activation (5, 6). LAIR2 can interact with complement component 1q (C1q) and mannose-binding lectin (MBL) and act as a complement inhibitor for the treatment and prevention of antibody-mediated allograft rejection and antibody-mediated clinical conditions (7).

References

  1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
  2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.
  3. Meyaard, L. et al. (1997) Immunity 7:283.
  4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.
  5. Lebbink, R.J. et al. (2008) J. Immunol. 180:1662.
  6. Olde Nordkamp, M.J. et al. (2011) Arthritis Rheum. 63:3749.
  7. Olde Nordkamp, M.J. et al. (2014) J. Innate Immun. 6:284.

Long Name

Leukocyte-associated Ig-like Receptor 2

Alternate Names

CD306

Entrez Gene IDs

3904 (Human); 102141809 (Cynomolgus Monkey)

Gene Symbol

LAIR2

UniProt

Product Documents for Recombinant Human LAIR2 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LAIR2 Fc Chimera Protein, CF

For research use only

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