Recombinant Human LAIR2 Fc Chimera Protein, CF

Name: Recombinant Human LAIR2 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10166-LR
Rating: 4 (1 reviews)

Catalog # 10166-LR

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Key Product Details

Source:	HEK293
Accession #:	Q6ISS4
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human LAIR2 Fc Chimera Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human LAIR2 protein

Human LAIR2 (Gln22-Pro152) Accession # Q6ISS4	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained: Glu23 inferred from enzymatic pyroglutamate treatment revealing Gln22

Predicted Molecular Mass

41 kDa

SDS-PAGE

50-56 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Human LAIR2 Fc Chimera binds with an ED₅₀ of 0.04-0.32 ng/mL.

Recombinant Human LAIR2 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Human LAIR2 Fc Chimera Protein Binding Activity When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Human LAIR2 Fc Chimera (Catalog # 10166-LR) binds with an ED₅₀ of 0.04-0.32 ng/mL.
	Recombinant Human LAIR2 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human LAIR2 Fc Chimera (Catalog # 10166-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-56 kDa and 100-120 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10166-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Desired Reconstitution Concentration

Background: LAIR2

LAIR2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR1, its transmembrane counterpart, LAIR2 shares 83% aa identity across the signal sequence and extracellular domains (1-3). Although one is secreted and the other is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR2 may compete with LAIR1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C-terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported. LAIR2 is a soluble collagen-receptor, and it can be detected in the synovial fluid of rheumatoid arthritis patients, urine of pregnant women, and as well as primary cells (5, 6). In vitro studies have demonstrated LAIR2 can compete with LAIR1 for the same collagen binding site and suggesting LAIR2 may play an important role in immune cell activation (5, 6). LAIR2 can interact with complement component 1q (C1q) and mannose-binding lectin (MBL) and act as a complement inhibitor for the treatment and prevention of antibody-mediated allograft rejection and antibody-mediated clinical conditions (7).

Item	Value
References	Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330. Meyaard, L. et al. (1999) J. Immunol. 162:5800. Meyaard, L. et al. (1997) Immunity 7:283. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553. Lebbink, R.J. et al. (2008) J. Immunol. 180:1662. Olde Nordkamp, M.J. et al. (2011) Arthritis Rheum. 63:3749. Olde Nordkamp, M.J. et al. (2014) J. Innate Immun. 6:284.
Long Name	Leukocyte-associated Ig-like Receptor 2
Entrez Gene IDs	3904 (Human); 102141809 (Cynomolgus Monkey)
Alternate Names	CD306, CD306 antigen, CD306leukocyte-associated Ig-like receptor-2, LAIR-2, LAIR2, MGC71634, leukocyte-associated Ig-like receptor 2, leukocyte-associated immunoglobulin-like receptor 2

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Recombinant Human LAIR2 Fc Chimera Protein, CF

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Key Product Details

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Product Specifications

Recombinant Human LAIR2 Fc Chimera Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: LAIR2

Citations for Recombinant Human LAIR2 Fc Chimera Protein, CF

Customer Reviews (1)

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Recombinant Human LAIR2 Fc Chimera Protein, CF

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