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Recombinant Human LAIR1 His Tagged Protein, CF

Catalog # 2664-LR | R&D Systems, Inc. a Bio-Techne Brand
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2664-LR-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human LAIR1 protein
Gln22-His163, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln22 predicted, sequencing might be blocked

Predicted Molecular Mass

16.3 kDa

SDS-PAGE

28-33 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.
The ED50 for this effect is 0.25-1.0 μg/mL.

Formulation, Preparation and Storage

2664-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR1

LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily (1-4). LAIR1 is a collagen-binding protein that is expressed in a differentiation- and activation-dependent manner on most immune cells, including T, B, NK and dendritic cells (DC), monocytes, CD34+ hematopoietic progenitors, most thymocytes, and selected granulocyte populations (2-7). Mature human LAIR1 is a 266 amino acid (aa) type I transmembrane protein that includes a 144 aa extracellular domain (ECD) with one collagen-binding C2-type Ig-like domain, and a 101 aa cytoplasmic domain with two ITIM motifs (2, 3, 8, 9). Of four potential human LAIR1 splice variants, LAIR1b has a 17 aa deletion within the ECD, but outside the Ig domain. LAIR1c differs from LAIR1b by one aa. LAIR1d has a 78 aa cytoplasmic truncation and lacks ITIM motifs. Human LAIR1 ECD shares <45% aa sequence identity with mouse, rat, bovine or canine LAIR1 ECD, but all are functional orthologs. Humans, but not rodents, also express the 152 aa secreted protein LAIR2, which shares 83% aa sequence identity with the LAIR1 ECD up to aa 140 and can block LAIR1 collagen binding (1, 2). A soluble form of LAIR1 found in plasma and urine also binds collagen (10).  Adhesion of LAIR1 to collagens in the extracellular matrix, transmembrane collagens expressed by tumor cells, or antibody-mediated crosslinking of LAIR1, inhibits signals relayed by ITAM-bearing receptors and some cytokine-mediated signals (6-8, 13).  Processes that are inhibited include B and T cell receptor-mediated activation, NK and T cell‑mediated cytotoxicity, and basophil degranulation (1-4, 8). LAIR1 is reduced or absent on chronic lymphocytic leukemia (CLL) B cells, and some B and DC cells in systemic lupus erythematosus (SLE). Its under‑expression potentially enhances CLL proliferation and SLE immune responses (7, 11, 12).

References

  1. Meyaard, L. (2008) J. Leukoc. Biol. 83:799.
  2. Meyaard, L. et al. (1997) Immunity 7:283.
  3. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535.
  4. Ouyang, W. et al. (2003) Biochem. Biophys. Res. Commun. 310:1236.
  5. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828.
  6. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419.
  7. Bonaccorsi, I. et al. (2010) PLoS ONE 5:e15080.
  8. Tang, X. et al. (2009) J. Immunol. 182:5446.
  9. Brondijk, T.H.C. et al. (2010) Blood 115:1364.
  10. Olde Nordkamp, M.J. et al. (2011) Arthritis Rheum. 63:3749.
  11. Poggi, A. et al. (2008) Leukemia 22:980.
  12. Colombo, B.M. et al. (2012) PLoS ONE 7:e31903.
  13. Rygiel, T.P. et al. (2011) Mol. Immunol. 49(1-2):402.

Long Name

Leukocyte-associated Ig-like Receptor 1

Alternate Names

CD305, HLAIR1, LAIR-1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Gene Symbol

LAIR1

UniProt

Additional LAIR1 Products

Product Documents for Recombinant Human LAIR1 His Tagged Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human LAIR1 His Tagged Protein, CF

For research use only

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  • Recombinant Human LAIR1 His Tagged Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
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