Recombinant Human LAIR1 Fc Chimera Protein, CF

Name: Recombinant Human LAIR1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 9964-LR
Rating: 4 (1 reviews)

Catalog # 9964-LR | R&D Systems, Inc. a Bio-Techne Brand

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Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	NP_002278
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human LAIR1 Fc Chimera Protein, CF

Product Specifications for Recombinant Human LAIR1 Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human LAIR1 protein

Human LAIR1 (Gln22-His163) Accession # NP_002278	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained. Gln22 inferred from enzymatic pyroglutamate treatment revealing Glu23.

Predicted Molecular Mass

42 kDa

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, 100 μL/well, Recombinant Human LAIR1 Fc Chimera binds with an ED₅₀ of 25-150 ng/mL.

Scientific Data Examples for Recombinant Human LAIR1 Fc Chimera Protein, CF

	Recombinant Human LAIR1 Fc Chimera Protein Binding Activity When Bovine Collagen I is coated at 10 µg/mL, Recombinant Human LAIR-1 Fc Chimera binds with an ED₅₀ of 25-150 ng/mL.
	Recombinant Human LAIR1 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Human LAIR1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 55-65 kDa and 110-130 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9964-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAIR1

LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily (1-4). LAIR1 is a collagen-binding protein that is expressed in a differentiation- and activation-dependent manner on most immune cells, including T, B, NK and dendritic cells (DC), monocytes, CD34⁺ hematopoietic progenitors, most thymocytes, and selected granulocyte populations (2-7). Mature human LAIR1 is a 266 amino acid (aa) type I transmembrane protein that includes a 144 aa extracellular domain (ECD) with one collagen-binding C2-type Ig-like domain, and a 101 aa cytoplasmic domain with two ITIM motifs (2, 3, 8, 9). Of four potential human LAIR1 splice variants, LAIR1b has a 17 aa deletion within the ECD, but outside the Ig domain. LAIR1c differs from LAIR1b by one aa. LAIR1d has a 78 aa cytoplasmic truncation and lacks ITIM motifs. Human LAIR1 ECD shares <45% aa sequence identity with mouse, rat, bovine or canine LAIR1 ECD, but all are functional orthologs. Humans, but not rodents, also express the 152 aa secreted protein LAIR2, which shares 83% aa sequence identity with the LAIR1 ECD up to aa 140 and can block LAIR1 collagen binding (1, 2). A soluble form of LAIR1 found in plasma and urine also binds collagen (10). Adhesion of LAIR1 to collagens in the extracellular matrix, transmembrane collagens expressed by tumor cells, or antibody-mediated crosslinking of LAIR1, inhibits signals relayed by ITAM-bearing receptors and some cytokine-mediated signals (6-8, 13). Processes that are inhibited include B and T cell receptor-mediated activation, NK and T cell‑mediated cytotoxicity, and basophil degranulation (1-4, 8). LAIR1 is reduced or absent on chronic lymphocytic leukemia (CLL) B cells, and some B and DC cells in systemic lupus erythematosus (SLE). Its under‑expression potentially enhances CLL proliferation and SLE immune responses (7, 11, 12).

Item	Value
References	Meyaard, L. (2008) J. Leukoc. Biol. 83:799. Meyaard, L. et al. (1997) Immunity 7:283. Lebbink, R.J. et al. (2004) J. Immunol. 172:5535. Ouyang, W. et al. (2003) Biochem. Biophys. Res. Commun. 310:1236. Verbrugge, A. et al. (2006) J. Leukoc. Biol. 79:828. Lebbink, R.J. et al. (2006) J. Exp. Med. 203:1419. Bonaccorsi, I. et al. (2010) PLoS ONE 5:e15080. Tang, X. et al. (2009) J. Immunol. 182:5446. Brondijk, T.H.C. et al. (2010) Blood 115:1364. Olde Nordkamp, M.J. et al. (2011) Arthritis Rheum. 63:3749. Poggi, A. et al. (2008) Leukemia 22:980. Colombo, B.M. et al. (2012) PLoS ONE 7:e31903. Rygiel, T.P. et al. (2011) Mol. Immunol. 49(1-2):402.
Long Name	Leukocyte-associated Ig-like Receptor 1
Entrez Gene IDs	3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)
Alternate Names	CD305, CD305 antigen, CD305leukocyte-associated Ig-like receptor 1, HLAIR1, LAIR-1, LAIR1, leukocyte-associated immunoglobulin-like receptor 1

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