Recombinant Human JAM-A Fc Chimera Protein, CF

Name: Recombinant Human JAM-A Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1103-JM

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1103-JM

Citations (5)

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Key Product Details

Source:	NS0
Accession #:	Q9Y624
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human JAM-A Fc Chimera Protein, CF

Product Specifications for Recombinant Human JAM-A Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human JAM-A protein

Human JAM-A (Ser28 - Ala242) Accession # Q9Y624	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser28

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1103-JM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: JAM-A

The family of junctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1) and platelet F11 receptor (3), is predominantly expressed at intercellular junctions of both epithelial cells and endothelial cells (1 - 4). It is also expressed on circulating blood cells including neutrophils, monocytes, platelets, erythrocytes and lymphocytes (5). Human JAM-A cDNA predicts a 299 amino acid (aa) residue precursor protein with a putative 27 aa signal peptide, a 210 aa extracellular region containing two Ig-like V-subset domains, a 24 aa transmembrane domain and a 38 aa cytoplasmic domain. The human and mouse proteins share approximately 67% aa sequence homology. Human JAM-A also shares approximately 35% and 32% aa sequence homology with human JAM-B and JAM-C, respectively. JAM-A exhibits homophilic interactions to regulate tight junction assembly and modulate paracellular permeability. This homophilic interation also mediates platelet aggregation and adhesion to endothelial cells and may play a role in thrombosis (3). JAM-A binds heterotypically with the beta 2 integrin lymphocyte function-associated antigen-1 (LFA-1). This JAM-A-LFA-1 interaction is involved in leukocyte adhesion and transmigration (6). JAM-A has also been shown to bind reovirus attachment protein sigma-1 to permit reovirus infection and signal virus-induced apoptosis (7).

Item	Value
References	Chavakis, T. et al. (2003) Thromb. Haemost. 89:13. Aurand-Lions, M. et al. (2001) Blood 98:3699. Sobocka, M.B. et al. (2000) Blood 95:2600. Martin-Padura, I. et al. (1998) J. Cell Biol. 142:117. Williams, L.A. et. al. (1999) Mol. Immunol. 36:1175. Ostermann, G. et al. (2002) Nature Immunol. 3:151. Barton, E.S. et al. (2001) Cell 104:441.
Long Name	Junctional Adhesion Molecule A
Entrez Gene IDs	50848 (Human); 16456 (Mouse); 101925695 (Cynomolgus Monkey)
Alternate Names	CD321, CD321 antigen, F11 receptor, F11R, JAM-1, JAM-A, JAM1CD321, JAMA, JCAMJAM, Junctional adhesion molecule 1Platelet F11 receptor, PAM-1, PAM-1KAT, Platelet adhesion molecule 1, junctional adhesion molecule A

Citations for Recombinant Human JAM-A Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human JAM-A Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

E Walker et al. (2021), Development of near-infrared imaging agents for detection of junction adhesion molecule-A protein Translational Oncology, 14(3):101007.
PMID: 33421750
Species: Human
Sample Types: Plasma
Applications: Standard

Williams D et al. (2015), JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals. J Leukoc Biol, 97(2):401-12.
PMID: 25420915
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Haarmann A et al. (2010), Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown. PLoS ONE, 5(10):e13568.
PMID: 21060661
Species: Human
Sample Types: Recombinant Protein
Applications: Enzyme Assay

Koenen RR et al. (2009), Regulated release and functional modulation of junctional adhesion molecule A by disintegrin metalloproteinases. Blood, 113(19):4799-809.
PMID: 19258599
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Miljkovic-Licina M et al. (2009), Sushi repeat protein X-linked 2, a novel mediator of angiogenesis. FASEB J., 23(12):4105-16.
PMID: 19667118

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Showing 1 - 5 of 5 citations Showing All

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Recombinant Human JAM-A Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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