Skip to main content

Novus Biologicals products are now on

Better as one

Recombinant Human Jagged 2 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1726-JG

Catalog #
Size / Price

Key Product Details



Accession #

Structure / Form

Disulfide-linked homodimer





Product Specifications


Chinese Hamster Ovary cell line, CHO-derived human Jagged 2 protein
Human Jagged 2
Accession #AAB61285
N-terminus C-terminus


>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis


Predicted Molecular Mass

138.7 kDa (monomer)


165-175 kDa, reducing conditions


Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842.
The ED50 for this effect is 0.5-2 µg/mL.

Formulation, Preparation and Storage

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 2

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1 - 5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor (SwissProt # Q9Y219). It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421 - 461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).


  1. Shawber, C. et al. (1996) Dev. Biol. 180:370.
  2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
  3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
  4. Schickwann, T. et al. (2000) Blood 96:950.
  5. DeHart, S. et al. (2005) Blood 105:3521.
  6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

Alternate Names


Entrez Gene IDs

3714 (Human); 16450 (Mouse); 29147 (Rat)

Gene Symbol



Additional Jagged 2 Products

Product Documents for Recombinant Human Jagged 2 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Jagged 2 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human Jagged 2 Fc Chimera Protein, CF (14)

Showing  1 - 10 of 14 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Jagged 2 Fc Chimera Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 10 of 14 publications Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.