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Recombinant Human ITIH5 His-tag Protein, CF

Catalog # 11009-IT | R&D Systems, Inc. a Bio-Techne Brand
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11009-IT-050

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ITIH5 protein
Ser17-Leu942 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser17

Predicted Molecular Mass

104 kDa

SDS-PAGE

110-122 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ITIH5 His-tag Protein is coated at 2.00 μg/mL (100 μL/well), it binds to Recombinant Human TSG-6  (Catalog # 2104-TS). The ED50 for this binding is 1.00-10.0 μg/mL.

Scientific Data Images for Recombinant Human ITIH5 His-tag Protein, CF

Recombinant Human ITIH5 His-tag Protein Binding Activity.

When Recombinant Human ITIH5 His-tag Protein (Catalog # 11009-IT) is coated at 2.00 μg/mL (100 μL/well), it binds to Recombinant Human TSG-6 (2104-TS). The ED50 for this binding is 1.00‑10.0 μg/mL.

Recombinant Human ITIH5 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human ITIH5 His-tag Protein (Catalog # 11009-IT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110‑122 kDa.

Formulation, Preparation and Storage

11009-IT
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ITIH5

Inter-alpha-trypsin inhibitor heavy chain 5 (ITIH5) is a heavy chain (HC) member of the ITIH family with predominant expression in the placenta, mammary gland, and ovary (1). It is composed of an N-terminal signal sequence, vault protein inter‑alpha‑trypsin domain (VIT), von-Willebrand type A domain, C-terminal cleavage site and multicopper oxidase domain conserved within other HCs in the family (1). The cleavage site present in ITIH members is processed during chain assembly (1) to reveal a C-terminal aspartic acid residue that enables crosslinking of the HC directly to chondroitin sulfate (CS) and hence to bikunin (2, 3). ITIHs can alternatively be associated with hyaluronan (HA) to form a Serum-derived hyaluronan associated protein (SHAP)-hyaluronan (HA) complex known to assist in stabilizing HA-rich extracellular matrices in inflammatory processes (3, 4). ITIH5 has been reported to modulate inflammation in skin disease and obesity and was suggested to play an important role more broadly in inflammation through its interaction with HA (5, 6). Tumor necrosis factor-stimulated gene 6 (TSG-6)-mediates interactions with the ITIH5 to enable the transfer of HC to HA matrix by forming a TSG-6:HC complex (7,8) that can facilitate Tumor Growth Factor beta1 (TGF beta1)‑dependent myofibroblast differentiation (9). ITIH5 has also been reported as downregulated or epigenetically silenced in several invasive cancers with poor prognosis including lung adenocarcinoma, breast cancer, gastric cancer, bladder cancer, and melanoma highlighting its potential as a therapeutic target (1, 10‑12).

References

  1. Himmelfarb, M. et al. (2004) Cancer Lett. 204:69.
  2. Salier, J.P. et al. (1996) Biochem. J. 315:1.
  3. Zhuo, L. et al. (2004) J. Biol. Chem. 279:38079.
  4. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
  5. Anveden, A. et al. (2012) Obesity 20:708.
  6. Huth, S. et al. (2020) Skin Pharmacol. Physiol. 33:198.
  7. Zhuo, L. et al. (2006) J. Biol. Chem. 281:20303.
  8. Baranova, N.S. et al. (2013) J. Biol. Chem. 288:29642.
  9. Martin, J. et al. (2016) J. Biol. Chem. 291:13789.
  10. Mai, C. et al. (2014) Med. Oncol. 31:53.
  11. Dotsch, M. et al. (2015) Epigenetics. 10:903.
  12. Rose, M. et al. (2017) Mol. Cancer 16:44.

Long Name

Inter-alpha-Trypsin Inhibitor Heavy Chain H5

Alternate Names

KIAA1953, MGC10848, PP14776

Entrez Gene IDs

80760 (Human)

Gene Symbol

ITIH5

UniProt

Additional ITIH5 Products

Product Documents for Recombinant Human ITIH5 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ITIH5 His-tag Protein, CF

For research use only

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