Source	Chinese Hamster Ovary cell line, CHO-derived human ITIH5 protein Ser17-Leu942 with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser17
Predicted Molecular Mass	104 kDa
SDS-PAGE	110-122 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Human ITIH5 His-tag Protein is coated at 2.00 μg/mL (100 μL/well), it binds to Recombinant Human TSG-6 (Catalog # 2104-TS). The ED₅₀ for this binding is 1.00-10.0 μg/mL.

Recombinant Human ITIH5 His-tag Protein, CF Scientific Data Examples

	Recombinant Human ITIH5 His-tag Protein Binding Activity. When Recombinant Human ITIH5 His-tag Protein (Catalog # 11009-IT) is coated at 2.00 μg/mL (100 μL/well), it binds to Recombinant Human TSG-6 (2104-TS). The ED₅₀ for this binding is 1.00‑10.0 μg/mL.
	Recombinant Human ITIH5 His-tag Protein SDS-PAGE. 2 μg/lane of Recombinant Human ITIH5 His-tag Protein (Catalog # 11009-IT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110‑122 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11009-IT

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 2 weeks, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

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Background: ITIH5

Inter-alpha-trypsin inhibitor heavy chain 5 (ITIH5) is a heavy chain (HC) member of the ITIH family with predominant expression in the placenta, mammary gland, and ovary (1). It is composed of an N-terminal signal sequence, vault protein inter‑alpha‑trypsin domain (VIT), von-Willebrand type A domain, C-terminal cleavage site and multicopper oxidase domain conserved within other HCs in the family (1). The cleavage site present in ITIH members is processed during chain assembly (1) to reveal a C-terminal aspartic acid residue that enables crosslinking of the HC directly to chondroitin sulfate (CS) and hence to bikunin (2, 3). ITIHs can alternatively be associated with hyaluronan (HA) to form a Serum-derived hyaluronan associated protein (SHAP)-hyaluronan (HA) complex known to assist in stabilizing HA-rich extracellular matrices in inflammatory processes (3, 4). ITIH5 has been reported to modulate inflammation in skin disease and obesity and was suggested to play an important role more broadly in inflammation through its interaction with HA (5, 6). Tumor necrosis factor-stimulated gene 6 (TSG-6)-mediates interactions with the ITIH5 to enable the transfer of HC to HA matrix by forming a TSG-6:HC complex (7,8) that can facilitate Tumor Growth Factor beta 1 (TGF beta 1)‑dependent myofibroblast differentiation (9). ITIH5 has also been reported as downregulated or epigenetically silenced in several invasive cancers with poor prognosis including lung adenocarcinoma, breast cancer, gastric cancer, bladder cancer, and melanoma highlighting its potential as a therapeutic target (1, 10‑12).

Item	Value
References	Himmelfarb, M. et al. (2004) Cancer Lett. 204:69. Salier, J.P. et al. (1996) Biochem. J. 315:1. Zhuo, L. et al. (2004) J. Biol. Chem. 279:38079. Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674. Anveden, A. et al. (2012) Obesity 20:708. Huth, S. et al. (2020) Skin Pharmacol. Physiol. 33:198. Zhuo, L. et al. (2006) J. Biol. Chem. 281:20303. Baranova, N.S. et al. (2013) J. Biol. Chem. 288:29642. Martin, J. et al. (2016) J. Biol. Chem. 291:13789. Mai, C. et al. (2014) Med. Oncol. 31:53. Dotsch, M. et al. (2015) Epigenetics. 10:903. Rose, M. et al. (2017) Mol. Cancer 16:44.
Long Name	Inter-alpha-Trypsin Inhibitor Heavy Chain H5
Entrez Gene IDs	80760 (Human)
Alternate Names	ITI heavy chain H5, ITI-HC5, ITIH5, ITIH5 inter-alpha-trypsin inhibitor heavy chain family, member 5, KIAA1953, MGC10848, PP14776, inter-alpha (globulin) inhibitor H5, inter-alpha-inhibitor heavy chain 5, inter-alpha-trypsin inhibitor heavy chain H5

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Recombinant Human ITIH5 His-tag Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

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Background: ITIH5

Citations for Recombinant Human ITIH5 His-tag Protein, CF

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