Recombinant Human Integrin alpha 4 beta 1 Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11719-A4

Fc Chimera
R&D Systems, part of Bio-Techne
Catalog #
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11719-A4-050
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Key Product Details

Source

HEK293

Structure / Form

Disulfide linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Integrin alpha 4 beta 1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Integrin alpha 4 beta 1 protein
Human ITGA4
(Tyr34-Gln970)
Accession # P13612.3		 IEGR Human IgG1
(Pro99-Lys330) (with modifications)
Human ITGB1
(Gln21-Asp728)
Accession # P05556.2		 IEGR Human IgG1
(Pro99-Lys330) (with modifications)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr34 (Integrin alpha 4) & Gln21 inferred from enzymatic pyroglutamate treatment revealing Thr22 (Integrin beta 1)

Predicted Molecular Mass

131 kDa (Integrin alpha 4) & 105 kDa (Integrin beta 1)

SDS-PAGE

128-155 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Integrin alpha 4 beta 1 Fc Chimera (Catalog # 11719-A4) binds to Recombinant Human VCAM-1/CD106 His-tag (Catalog # 10201-VC) with an ED50 of <2.00 µg/mL.

Scientific Data Images for Recombinant Human Integrin alpha 4 beta 1 Fc Protein, CF

Recombinant Human Integrin alpha 4 beta 1 Fc Chimera Protein Binding Activity.

Recombinant Human Integrin alpha 4 beta 1 Fc Chimera Protein (Catalog # 11719-A4) binds to Recombinant Human VCAM-1/CD106 His-tag (10201-VC) in a functional ELISA with an ED50 of < 2.00 µg/mL.

Recombinant Human Integrin alpha 4 beta 1 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human Integrin alpha 4 beta 1 Fc Chimera Protein (Catalog # 11719-A4) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 128-155 kDa.

Formulation, Preparation and Storage

11719-A4
Formulation Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Integrin alpha 4 beta 1

Integrin alpha4 beta1, also called VLA4, is an integrin family adhesion receptor that shares the beta1 subunit with eleven other family members and the alpha4 subunit with integrin alpha4 beta7 (1 - 4). The non-covalent heterodimer of 150 kDa alpha4/CD49d and 130 kDa beta1/CD29 type I transmembrane glycoprotein subunits mediates cell adhesion to VCAM-1/CD106 on other cells and the CS-1 fragment of fibronectin in the extracellular matrix (2 - 4). The alpha4 extracellular domain (ECD) contains an N-terminal beta-propeller structure, followed by domains termed thigh, calf-1 and calf-2 (1). The beta1 ECD contains a vWFA domain, which interacts with the alpha4 beta-propeller to form a binding domain when the dimer is in active, extended and open conformation. Each subunit has a transmembrane sequence and a short cytoplasmic tail. The 944 aa human alpha4 extracellular domain (ECD) shares 85% aa identity with mouse, rat and canine alpha4, while the 708 aa human beta1 ECD shares 92 - 96% aa identity with rat, bovine, mouse, and feline beta1. Five alternate splice forms of the human beta1 cytoplasmic domain, including one antagonistic form, vary by 12 to 48 aa and show differential expression patterns (5). Leukocytes (except for neutrophils), erythroid precursors and some non-hematopoietic cells such as epicardial, endothelial and smooth muscle precursors, Schwann cells, and chorionic cells express alpha4 beta1 (6 - 10). Deletion is lethal in the mouse embryo due to faulty placentation and development of the epicardium and coronary vessels (7, 10). In the adult, alpha4 beta1 primarily regulates immune cell migration (11 - 13). Circulating leukocyte alpha4 beta1 is rapidly activated by inflamed endothelial cells that present VCAM-1 and chemokines such as SDF-1 (11). This activation facilitates rolling, firm adhesion, and extravasation. Interfering with leukocyte migration via the therapeutic alpha4 beta1antibody Natalizumab can reduce the severity of autoimmune disorders such as multiple sclerosis (12). Natalizumab can also mobilize hematopoietic precursors from the bone marrow by impeding their interaction with stromal cell VCAM-1 (8, 12). During immune cell activation, alpha4 beta1 can function as a costimulatory molecule (13, 14).

References

  1. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  2. Takada, Y. et al. (1989) EMBO J. 8:1361.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183.
  4. Takada, Y. et al. (2007) Genome Biol. 8:215.
  5. Svineng, G. et al. (1998) Biochem. J. 330:1255.
  6. Eshghi, S. et al. (2007) J. Cell Biol. 177:871.
  7. Yang, J.T. et al. (1995) Development 121:549.
  8. Sheppard, A.M. et al. (1994) Cell Adhes. Commun. 2:27.
  9. Haack, H. and R.O. Hynes (2001) Dev. Biol. 233:38.
  10. Grazioli, A. et al. (2006) Dev. Biol. 293:165.
  11. Alon, R. and K. Ley (2008) Curr. Opin. Cell Biol. 20:525.
  12. Bauer, M. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1920.
  13. Banerjee, E.R. et al. (2008) Exp. Hematol. 36:1004.
  14. Nguyen, K. et al. (2008) Immunity 28:810.

Alternate Names

CD49d, IA4

Entrez Gene IDs

3676 (Human)

Gene Symbol

ITGA4

Additional Integrin alpha 4 beta 1 Products

Product Documents for Recombinant Human Integrin alpha 4 beta 1 Fc Protein, CF

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Product Specific Notices for Recombinant Human Integrin alpha 4 beta 1 Fc Protein, CF

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