Key Product Details
Met42-Leu1019, with an N-terminal Met and 7-His tag
N-terminal Sequence Analysis
Predicted Molecular Mass
The specific activity is >1,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
|Formulation||Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij-35 and Glycerol.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Insulysin, or insulin-degrading enzyme (IDE), is a zinc metallopeptidase of the inverzincin family. IDE is primarily located in the cytosol, but has been detected as a secreted enzyme and associated with the plasma membrane as well (1). The enzyme is expressed in many tissues, with the highest levels in liver, kidney, brain, and testis (2). IDE hydrolyzes a variety of regulatory peptides, including insulin, glucagon, atrial natriuretic factor, and transforming growth factor-alpha in vitro (1). In addition, IDE has been shown to degrade the amyloid beta (A beta) peptide, which polymerizes into the plaques associated with Alzheimer's disease (3). Deficiencies in IDE activity may contribute to the pathogenesis of type 2 diabetes mellitus (DM2) and Alzheimer's disease. The IDE region of human chromosome 10q has been genetically linked to DM2 (4). When the IDE gene was specifically disrupted in mice, IDE -/- animals developed hyperinsulinemia and glucose intolerance, characteristics of DM2 (5). The IDE -/- mice were also shown to have a significant decrease in A beta degradation in the brain, resulting in increased cerebral accumulation of A beta peptide. This in vivo evidence is consistent with the hypotheses that IDE is important for the degradation of insulin in cells and for the clearance of A beta peptide in the brain.
- Affholter, J. A. et al. (1988) Science 242:1415.
- Duckworth, W.C. et al. (1998) Endocr. Rev. 19:608.
- Akiyama, H. et al. (1990) Biochem. Biophys. Res. Commun. 170:1325.
- Selkoe, D.J. (2001) Neuron 32:177.
- Ghosh, S. et al. (2000) Am. J. Hum. Genet. 67:1174.
- Farris, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4162.
Product Specific Notices for Recombinant Human Insulysin/IDE Protein, CF
For research use only