Recombinant Human Inhibin A Protein

Name: Recombinant Human Inhibin A Protein
Brand: R&D Systems
SKU: 8506-AB
Rating: 4 (1 reviews)

Catalog # 8506-AB | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Source:	CHO
Accession #:	P05111 (alpha chain) & P08476 (beta chain)
Structure / Form:	Disulfide-linked heterodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human Inhibin A Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human Inhibin A protein Ser233-Ile366 (alpha chain) & Gly311-Ser426 (beta chain)
Purity	>95%, by SDS-PAGE with silver staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ser233 (alpha chain) & Gly311 (beta chain)
Predicted Molecular Mass	15 kDa (alpha chain) & 13 kDa (beta chain)
SDS-PAGE	12-25 kDa, reducing conditions
Activity	Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED₅₀for this effect is 3-18 ng/mL.

Scientific Data Examples for Recombinant Human Inhibin A Protein

	Recombinant Human Inhibin A Protein Bioactivity Recombinant Human Inhibin A (Cat# 8506-AB) neutralizes Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED₅₀ for this effect is 3-18 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

8506-AB

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8506-AB/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Inhibin A

Inhibin A is a member of the TGF-beta superfamily of proteins (1). Mature Inhibin A is a disulfide-linked dimer composed of alpha and beta subunits (1). Inhibin A shares a common alpha subunit with the closely related protein Inhibin B but has a unique beta subunit ( beta A) (2, 3). The mature alpha subunit of human Inhibin A has a predicted molecular weight of 18 kDa and shares 80% amino acid (aa) sequence identity with the mouse and rat orthologs (2, 4). The mature beta subunit of this human protein has a predicted molecular weight of 13 kDa and shares 100% aa sequence identity with the mouse and rat orthologs. Inhibin binds and antagonizes ActRIIA and ActRIIB in complex with the TGF-beta RIII and/or IGSF1 co-receptors and subsequently acts to suppress Activin-induced Follicle Stimulating Hormone (FSH) secretion (1, 5-7). Inhibins are produced by gonadal cells in both males and females (8). They are thought to be involved in the regulation of gametogenesis, and embryonic and fetal development (8, 9). Elevated concentrations of Inhibins are associated with pregnancy, preeclampsia, and ovarian cancer, and Inhibin A levels are typically measured during prenatal screening for Down’s syndrome (10-14).

Item	Value
References	Phillips, D.J. and T.K. Woodruff (2004) Growth Factors 22:13. Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957. Ying, S.Y. (1987) Proc. Soc. Exp. Biol. Med. 186:253. Mayo, K.E. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5849. Lewis, K.A. et al. (2000) Nature 404:411. Martens, J.W. et al. (1997) Endocrinology 138:2928. Chapman, S.C. et al. (2002) Mol. Cell. Endocrinol. 196:79. de Kretser, D.M. and D.M. Robertson (1989) Biol. Reprod. 40:33. Knight, P.G. et al. (2012) Mol. Cell. Endocrinol. 359:53. Walentowicz, P. et al. (2014) PLoS One 9:e90575. Kondi-Pafiti, A. et al. (2013) Clin. Exp. Obstet. Gynecol. 40:109. Kuijper, E.A. et al. (2013) Reprod. Biomed. Online 27:33. Carty, D.M. et al. (2008) Trends Cardiovasc. Med. 18:186. Wald, N.J. et al. (1996) Prenat. Diagn. 16:143.
Entrez Gene IDs	3623 (Human); 16322 (Mouse)
Alternate Names	A-inhibin subunit, INHA, Inhibin A, inhibin alpha chain, inhibin, alpha

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Recombinant Human Inhibin A Protein

Name: Pablo Hurtado

Application: CellProlif

Verified Customer | Posted 10/09/2020

Recombinant Human Inhibin A Protein

Catalog # 8506-AB | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human Inhibin A Protein

Scientific Data Examples for Recombinant Human Inhibin A Protein

Formulation, Preparation and Storage

Carrier Free

8506-AB

Carrier

8506-AB/CF

Carrier Free

Reconstitution Calculator

Background: Inhibin A

Citations for Recombinant Human Inhibin A Protein

Customer Reviews for Recombinant Human Inhibin A Protein (1)

Recombinant Human Inhibin A Protein

Product Documents for Recombinant Human Inhibin A Protein

Product Datasheets

COA

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