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Recombinant Human ILDR1 Fc Chimera Protein, CF

Catalog # 10152-D1 | R&D Systems, Inc. a Bio-Techne Brand
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10152-D1-050

Key Product Details

Source

HEK293

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ILDR1 protein
Human ILDR1
(Leu24-His167)
Accession # Q86SU0
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24

Predicted Molecular Mass

43 kDa

SDS-PAGE

40-49 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human ILDR1 Fc Chimera is immobilized at 2.5 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human ILDR2 Fc Chimera that produces 50% of the optimal binding response is 2-12 μg/mL

Scientific Data Images for Recombinant Human ILDR1 Fc Chimera Protein, CF

Recombinant Human ILDR1 Fc Chimera Protein SDS-PAGE

Recombinant Human ILDR1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human ILDR1 Fc Chimera (Catalog # 10152-D1) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-49 kDa and 80-100 kDa, respectively.

Formulation, Preparation and Storage

10152-D1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ILDR1

Immunoglobulin-like domain containing receptor 1, or ILDR1, is a single pass Type I membrane protein that belongs to the immunoglobulin superfamily. Human ILDR1 contains a signal peptide (aa 1-23), an Ig-like V-type extracellular domain (aa 24-167), a helical transmembrane domain amino acids (aa168-188), and a cytoplasmic domain (aa 189-546) that contains a cysteine rich region and an arginine rich region (1). The extracellular domain of human ILDR1 shares a 96.5% homology with mouse and rat respectively. ILDR1 is mainly expressed in the prostate, with lower levels also found in testis, pancreas, heart and liver (1). ILDR1 has also been found expressed by intestinal cholecystokinin (CCK)-producing cells (2). ILDR1 has three splice variants. Two of those are expressed on the cell surface, while one is expressed in the cytoplasm, except when co-expressed with one of the other two splice variants. The cytoplasmic variant is the shortest transcript and is not found in healthy tissue, but only in lymphoma samples (1). It is suggested that ILDR1 plays a role in regulating intestinal hormone levels by stimulating release of CCK in response to fatty acids (2). Loss of function of ILDR1 is associated with Autosomal-Recessive Hearing Impairment (3). Our data shows that ILDR1 binds to human immunoglobulin-like domain containing receptor 2 (ILDR2).

References

  1. Hauge, H. et al. (2004) Biochem. Biophys. Res. Commun. 323:970.
  2. Chandra, R. et al. (2013) J. Clin. Invest. 123:3343.
  3. Borck, G. et al. (2011) Am. J. Hum. Genet. 88:127.

Long Name

Immunoglobulin Like Domain Containing Receptor 1

Alternate Names

Angulin-2, DFNB42, ILDR1alpha, ILDR1beta

Entrez Gene IDs

286676 (Human); 106347 (Mouse); 303914 (Rat)

Gene Symbol

ILDR1

UniProt

Product Documents for Recombinant Human ILDR1 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ILDR1 Fc Chimera Protein, CF

For research use only

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