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Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)

Bio-Techne includes R&D Systems | Catalog # 618-IL

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Carrier Free
618-IL-010/CF
618-IL-050/CF

Catalog #
Availability
Size / Price
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With Carrier
618-IL-010
618-IL-050

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human IL-8/CXCL8 protein
Ala23-Ser99 & Ser28-Ser99

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23 & Ser28

Predicted Molecular Mass

8.9 kDa

Activity

Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474.
The ED50 for this effect is 0.2-0.4 µg/mL.

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2.
The ED50 for this effect is 1-5 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 618-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 618-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties (1). CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4 (2), and it can also interact with matrix and cell surface glycosaminoglycans (3). Mature human CXCL8 shares 65%-69% amino acid (aa) sequence identiity with canine, feline, and porcine CXCL8 (4). There is no CXCL8 gene counterpart in rodent. N-terminal truncation by multiple proteases generates a range of shorter forms, and an alternative splice form of human CXCL8 carries an eleven aa substitution at the C-terminus (5). The bioactivity of CXCL8 is regulated by these truncations, by CXCL8 citrullination at Arg5 (N-terminal to the ELR motif) (6), and by the decoy receptor DARC (7). CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). CXCR1 and CXCR2 associate into functional homodimers and heterodimers with each other (8). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (9). It triggers the antimicrobial activation of neutrophils through CXCR1 (10). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents CXCL8 interaction with CXCR1 (11). CXCL8 is upregulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (12). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (13-16).

References

  1. Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133.
  2. Nesmelova, I.V. et al. (2005) J. Biol. Chem. 280:4948.
  3. Pichert, A. et al. (2012) Biomatter 2:142.
  4. Schmid, J. and C. Weissmann (1987) J. Immunol. 139:250.
  5. Mortier, A. et al. (2008) Pharmacol. Ther. 120:197.
  6. Proost, P. et al. (2008) J. Exp. Med. 205:2085.
  7. Neote, K. et al. (1994) Blood 84:44.
  8. Munoz, L.M. et al. (2009) J. Immunol. 183:7337.
  9. Gerszten, R.E. et al. (1999) Nature 398:718.
  10. Jones, S.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93:6682.
  11. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236.
  12. Apostolakis, S. et al. (2009) Cardiovasc. Res. 84:353.
  13. Martin, D. et al. (2009) J. Biol. Chem. 284:6038.
  14. Li, A. et al. (2005) Angiogenesis 8:63.
  15. Waugh, D.J. and C. Wilson (2008) Clin. Cancer Res. 14:6735.
  16. Fernando, R.I. et al. (2011) Cancer Res. 71:5296.

Long Name

Interleukin 8

Alternate Names

CXCL8, GCP1, IL8, LAI, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, NCF, TCF, TSG1

Entrez Gene IDs

3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)

Gene Symbol

CXCL8

UniProt

Additional IL-8/CXCL8 Products

Product Documents for Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)

For research use only

Citations for Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived) (5)

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Customer Reviews for Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived) (2)

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  • Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 11/15/2023
    Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived) 618-IL
  • Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)
    Name: Anonymous
    Application: Sprouting angiogenesis
    Verified Customer | Posted 07/25/2023
    Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived) 618-IL

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FAQs for Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)

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  • Q: Are mouse or rat CXCL8/IL-8 products offered?

    A: Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

  • Q: What is the difference between 208-IL (rhCXCL8/IL-8) and  618-IL (rhEndothelial Cell-Derived IL-8/CXCL8)?

    A: Several N-terminal processed forms of CXCL8/IL-8 are produced by proteolytic cleavage after secretion by cells such as peripheral blood monocytes, leukcocytes and endothelial cells. Catalog # 208-IL consists only of the sequence Ser28 to Ser99 (also known as IL-8, 6-77), which is the most prominent form of IL-8. According to literature, IL-8(6-77) has a 5-10-fold higher activity in neutrophil activation. Catalog # 618-IL consists of Ser28 to Ser99 (IL-8, 6-77) and Ala23 to Ser99 (IL-8).  In our chemoattraction assay, 208-IL has a 2-fold higher activity than 618-IL.Several N-terminal processed forms of CXCL8/IL-8 are produced by proteolytic cleavage after secretion by cells such as peripheral blood monocytes, leukcocytes and endothelial cells. Catalog # 208-IL consists only of the sequence Ser28 to Ser99 (also known as IL-8, 6-77), which is the most prominent form of IL-8. According to literature, IL-8(6-77) has a 5-10-fold higher activity in neutrophil activation. Catalog # 618-IL consists of Ser28 to Ser99 (IL-8, 6-77) and Ala23 to Ser99 (IL-8).  In our chemoattraction assay, 208-IL has a 2-fold higher activity than 618-IL.

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Reconstitution Calculator

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