Recombinant Human IL-4R alpha Fc Chimera Protein, CF

Name: Recombinant Human IL-4R alpha Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 7700-4R
Rating: 3 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7700-4R

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Key Product Details

Source:	HEK293
Accession #:	P24394
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human IL-4R alpha Fc Chimera Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human IL-4 R alpha protein

Human IL-4 R alpha (Gly24-His232) Accession # P24394	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE with silver staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly24

Predicted Molecular Mass

51 kDa (monomer)

SDS-PAGE

65-90 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 4-20 ng/mL in the presence of 0.2 ng/mL of Recombinant Human IL-4 (Catalog # 204-IL).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7700-4R

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-4R alpha

Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4 Ra plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1-5). Mature human IL-4 Ra consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6, 7). Within the ECD, human IL-4 Ra shares 51% aa sequence identity with mouse and rat IL-4 Ra. Soluble forms of IL-4 Ra, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity (8-11). IL-4 Ra is a component of two distinct receptor complexes and shows species selectivity between human and mouse (6). It can associate with the common gamma chain ( gamma c) to form the IL-4 responsive type I receptor in which gamma c increases the affinity for IL-4 and enables signaling (12, 13). It can alternatively associate with IL-13 Ra1 to form the type II receptor which is responsive to both IL-4 and IL-13 (14, 15). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize gamma c (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (16, 17).

Item	Value
References	Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55. Gordon, S. and F.O. Martinez (2010) Immunity 32:593. Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384. Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415. Lee, Y.W. et al. (2010) Biomol. Ther. 18:135. Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861. Galizzi, J.P. et al. (1990) Int. Immunol. 2:669. Kruse, S. et al. (1999) Int. Immunol. 11:1965. Blum, H. et al. (1996) J. Immunol. 157:1846. Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23. Mosley, B. et al. (1989) Cell 59:335. Kondo, M. et al. (1993) Science 262:1874. Russell, S.M. et al. (1993) Science 262:1880. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265. Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25. Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.
Long Name	Interleukin 4 Receptor alpha
Entrez Gene IDs	3566 (Human); 16190 (Mouse)
Alternate Names	CD124, CD124 antigen, IL-4 R alpha, IL-4 receptor subunit alpha, IL-4R alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4Ra, IL4R, IL4R alpha, IL4RACD124, interleukin 4 receptor, interleukin-4 receptor alpha chain, interleukin-4 receptor subunit alpha

Citations for Recombinant Human IL-4R alpha Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-4R alpha Fc Chimera Protein, CF include:

Species: Human
Sample Types: Peptide
Applications: Binding Assay

LL Eggink et al. (2018), An innovative immunotherapeutic strategy for ovarian cancer: CLEC10A and glycomimetic peptides J Immunother Cancer, 6(1):28.
PMID: 29665849

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Recombinant Human IL-4 R alpha Fc Chimera Protein, CF

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Application: In vitro bioactivity in cell culture

Verified Customer | Posted 12/06/2017

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