Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF

Name: Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI7700

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AVI7700

Product Datasheet / COA / SDS

Biotinylated

Catalog #

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Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	P24394.1
Structure / Form:	Disulfide-linked homodimer, Biotinylated via Avi-tag
Applications:	Binding Activity, Bioactivity

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Product Specifications for Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF

Source

Human embryonic kidney cell, HEK293-derived human IL-4R alpha protein

Human IL-4R alpha
(Gly24-His232)
Accession # P24394.1

IEGRMD

Human IgG₁
(Pro100-Lys330)

Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Gly24

Predicted Molecular Mass

52 kDa

SDS-PAGE

73-82 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 5-30 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL‑4 (Catalog # 204-IL). Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human IL-4R alpha Fc Chimera Avi-tag (Catalog # AVI7700) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004), the concentration of Recombinant Human IL-4 (Catalog # 204-IL) that produces 50% of the optimal binding response is approximately 3-20 ng/mL.

Scientific Data Examples for Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein, CF

	Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein Binding Activity When Biotinylated Recombinant Human IL-4R alpha Fc Chimera Avi-tag (Catalog # AVI7700) is captured on EvenCoat Streptavidin Coated Plates (Catalog # CP004), the concentration of Recombinant Human IL-4 (Catalog # 204-IL) that produces 50% of the optimal binding response is approximately 3-20 ng/mL.
	Recombinant Human IL-4R alpha Fc Chimera Avi-tag Protein SDS-PAGE 2 μg/lane of Biotinylated Recombinant Human IL-4R alpha Fc Chimera Avi-tag (Catalog # AVI7700) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-82 kDa and 145 -165 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI7700

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-4R alpha

Interleukin 4 Receptor alpha (IL-4R alpha ), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1-5). Mature human IL-4R alpha consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6, 7). Within the ECD, human IL-4R alpha shares 51% aa sequence identity with mouse and rat IL-4R alpha. Soluble forms of IL-4R alpha, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity (8-11). IL-4R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (6). It can associate with the common gamma chain ( gamma c) to form the IL-4 responsive type I receptor in which gamma c increases the affinity for IL-4 and enables signaling (12, 13). It can alternatively associate with IL-13R alpha 1 to form the type II receptor which is responsive to both IL-4 and IL-13 (14, 15). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize gamma c (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (16, 17).

Item	Value
References	Wills-Karp, M. and F.D. Finkelman (2008) Sci. Signal. 1:pe55. Gordon, S. and F.O. Martinez (2010) Immunity 32:593. Kuperman, D.A. and R.P. Schleimer (2008) Curr. Mol. Med. 8:384. Li, Z. et al. (2009) Cell. Mol. Immunol. 6:415. Lee, Y.W. et al. (2010) Biomol. Ther. 18:135. Idzerda, R.L. et al. (1990) J. Exp. Med. 171:861. Galizzi, J.P. et al. (1990) Int. Immunol. 2:669. Kruse, S. et al. (1999) Int. Immunol. 11:1965. Blum, H. et al. (1996) J. Immunol. 157:1846. Jung, T. et al. (1999) Int. Arch. Allergy Immunol. 119:23. Mosley, B. et al. (1989) Cell 59:335. Kondo, M. et al. (1993) Science 262:1874. Russell, S.M. et al. (1993) Science 262:1880. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. 93:497. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265. Ramalingam, T.R. et al. (2008) Nat. Immunol. 9:25. Overwijk, W.W. and K.S. Schluns (2009) Clin. Immunol. 132:153.
Long Name	Interleukin 4 Receptor alpha
Entrez Gene IDs	3566 (Human); 16190 (Mouse)
Alternate Names	CD124, CD124 antigen, IL-4 R alpha, IL-4 receptor subunit alpha, IL-4R alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4Ra, IL4R, IL4R alpha, IL4RACD124, interleukin 4 receptor, interleukin-4 receptor alpha chain, interleukin-4 receptor subunit alpha

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