Recombinant Human IL-4 (CHO-expressed) Protein

Name: Recombinant Human IL-4 (CHO-expressed) Protein
Brand: R&D Systems
SKU: 6507-IL
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 6507-IL

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Key Product Details

Source:	CHO
Accession #:	P05112
Structure / Form:	Monomer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-4 (CHO-expressed) Protein

Product Specifications for Recombinant Human IL-4 (CHO-expressed) Protein

Source	Chinese Hamster Ovary cell line, CHO-derived human IL-4 protein His25-Ser153
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His25
Predicted Molecular Mass	15.0 kDa
SDS-PAGE	17-25 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.05-0.2 ng/mL.

Scientific Data Examples for Recombinant Human IL-4 (CHO-expressed) Protein

	Recombinant Human IL-4 (CHO-expressed) Protein Bioactivity Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.05-0.2 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

6507-IL

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6507-IL/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1 ‑ 3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5 - 7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10 ‑ 13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

Item	Value
References	Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894. Redfield, C. et al. (1991) Biochemistry 30:11029. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451. Mosman, T.R. et al. (1987) J. Immunol. 138:1813. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701. Paludan, S.R. (1998) Scand. J. Immunol. 48:459. Corthay, A. (2006) Scand. J. Immunol. 64:93. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name	Interleukin 4
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names	B cell growth factor 1, B-cell stimulatory factor 1, BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL-4B_cell stimulatory factor 1, IL4, IL4E12, Lymphocyte stimulatory factor 1, MGC79402, binetrakin, interleukin 4, interleukin-4, pitrakinra

Citations for Recombinant Human IL-4 (CHO-expressed) Protein (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-4 (CHO-expressed) Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Zhou et al. (2018), Exosomes released from tumor-associated macrophages transfer miRNAs that induce a Treg/Th17 cell imbalance in epithelial ovarian cancer Cancer Immunol Res, 0(0).
PMID: 30396909
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

R Bruen et al. (2017), Liraglutide dictates macrophage phenotype in apolipoprotein E null mice during early atherosclerosis Cardiovasc Diabetol, 16(1):143.
PMID: 29110715
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Matsubara K et al. (2015), Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity. J Neurosci, 35(6):2452-64.
PMID: 25673840

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Customer Reviews for Recombinant Human IL-4 (CHO-expressed) Protein (2)

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Recombinant Human IL-4 (CHO-expressed) Protein

Name: Jayalakshmi Miriyala

Application: Stem/Immune cell maintenance or differentiation

Reason for Rating: I used this to differentiate the monocytes to dendritic cells

Verified Customer | Posted 10/01/2020
Recombinant Human IL-4 (CHO-expressed) Protein

Name: Robert Berahovich

Application: In vitro bioactivity in cell culture

Reason for Rating: Excellent for inducing human monocyte maturation into dendritic cells (along with GM-CSF). Cells were cultured for 6 days with IL-4 at a final concentration of 35 ng/ml.

Verified Customer | Posted 09/29/2020

Flow cytometric of human monocytes (left) before and after differentiation into dendritic cells (right). CD1a-CD14+ monocytes are in the upper left quadrant, whereas CD1a+CD14- dendritic cells are in the lower right quadrant.

Recombinant Human IL-4 (CHO-expressed) Protein

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Human IL-4 (CHO-expressed) Protein

Scientific Data Examples for Recombinant Human IL-4 (CHO-expressed) Protein

Formulation, Preparation and Storage

Carrier Free

6507-IL

Carrier

6507-IL/CF

Carrier Free

Reconstitution Calculator

Background: IL-4

Citations for Recombinant Human IL-4 (CHO-expressed) Protein (3)

Customer Reviews for Recombinant Human IL-4 (CHO-expressed) Protein (2)

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Recombinant Human IL-4 (CHO-expressed) Protein

Recombinant Human IL-4 (CHO-expressed) Protein

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