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Recombinant Human IL-36 alpha/IL-1F6 Protein

Bio-Techne includes R&D Systems | Catalog # 1078-IL

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Carrier Free
1078-IL-025/CF

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With Carrier
1078-IL-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

E. coli-derived human IL-36 alpha/IL-1F6 protein
Met1-Phe158

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

17.7 kDa

Activity

Measured by its binding ability in a functional ELISA.
Immobilized recombinant human IL-36 alpha at 1 µg/mL (100 µL/well) can bind recombinant human IL-1 Rrp2 Fc Chimera (Catalog # 872-RP) with a linear range of 0.15-5 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1078-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Tween® 20 with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1078-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Tween® 20.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 alpha/IL-1F6

Human interleukin‑36 alpha [IL‑36 alpha; previously IL‑1F6 and also FIL‑1 epsilon (epsilon)] is a member of the IL‑1 family of proteins (1 ‑ 3, 6). IL‑1 family members include IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36Ra/IL‑1F5, IL‑37/IL‑1F7, IL‑36 beta/IL‑1F8, IL‑36 gamma/IL‑1F9 and IL‑1F10 (4, 6). All family members show a 12 beta‑strand, beta‑trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL‑36 alpha/IL‑1F6 is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation site(s) (1 ‑ 3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL‑1F6 has 54% aa identity. Within the family, IL‑1F6 is 30% aa identical to IL‑1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL‑1 beta, IL‑36Ra/IL‑1F5, IL‑37/IL‑1F7, IL‑36 beta/IL‑1F8, IL‑36 gamma/IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL‑36 alpha/IL‑1F6 include monocytes, B cells and T cells (1, 4). Notably, IL‑36 alpha/IL‑1F6 is the only novel IL‑1 family member found to be expressed on T cells. The receptor for IL‑36 alpha/IL‑1F6 is reported to be a combination of IL‑1 Rrp2 and IL‑1 RAcP (5). Recombinant IL‑36 alpha/IL‑1F6, along with IL‑36 beta/IL‑1F8 and IL‑36 gamma/IL‑1F9, has been shown to act as an agonist by activating the pathway involving NF‑ kappaB and MAPK in an IL‑1 Rrp2 dependent manner. This suggests that IL‑36 alpha/IL‑1F6 may signal in a similar fashion to IL‑1 and IL‑18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Alternate Names

FIL1 epsilon, IL-1E, IL1F6, IL36 alpha, IL36A

Entrez Gene IDs

27179 (Human); 54448 (Mouse)

Gene Symbol

IL36A

UniProt

Additional IL-36 alpha/IL-1F6 Products

Product Documents for Recombinant Human IL-36 alpha/IL-1F6 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-36 alpha/IL-1F6 Protein

Tween is a registered trademark of ICI Americas.

For research use only

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