Recombinant Human IL-22BP Fc Chimera Protein, CF

Name: Recombinant Human IL-22BP Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1087-BP

Catalog # 1087-BP

Citations (5)

Product Datasheet / COA / SDS

New Version Available - 8498-BP/CF

A New rh IL-22BP is Available! It exhibits higher purity, has greater than 4X the activity, and has a His-tag!

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Key Product Details

Source:	NS0
Accession #:	NP_851826
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-22BP protein

Human IL-22BP (Thr22-Pro231) Accession # NP_851826	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Thr22

Predicted Molecular Mass

51.3 kDa (monomer)

SDS-PAGE

75-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is 4-20 ng/mL in the presence of 1 ng/mL of Recombinant Human IL-22 (Catalog # 782-IL).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1087-BP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-22BP

Interleukin 22 binding protein (IL-22BP), also known as CRF2-10, CRF2-X, and IL-22 RA2, is a 35-45 kDa secreted glycoprotein in the type II cytokine receptor family (CRF). IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is also a component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29 (1, 2). IL-22BP blocks the interaction of IL-22 with IL-22 R, preventing IL-22 induced production of reactive oxygen species, IL-6, IL-10, and TNF-a (3-8). In vivo, it regulates the proinflammatory effects of IL-22 (e.g. neutrophil infiltration) but not of IL-10 (7). Mouse IL-22BP can neutralize the bioactivity of both mouse and human IL-22 (6). IL-22BP is produced by dendritic cells (DC), epithelial cells, activated B cells, and activated monocytes (3, 6, 9, 10). It is constitutively expressed by DC but is down-regulated during local inflammation and in response to tissue damage (11-13). IL-22BP is critical for limiting IL-22 induced epithelial cell proliferation during wound healing, and its deficiency can enable uncontrolled proliferation and enhance tumor development (12). Mature human IL-22BP contains two Fibronectin type-III domains (4, 6). Alternative splicing generates additional isoforms that contain a 32 amino acid (aa) insertion in the first Fn-III domain and may also be truncated within the second Fn-III domain (3, 4, 14). Human IL-22BP without the 32 aa insertion shares 68% and 73% amino acid (aa) sequence identity with mouse and rat IL-22BP, respectively.

Item	Value
References	Lim, C. and R. Savan (2014) Cytokine Growth Factor Rev. 25:257. Mizoguchi, A. (2012) Inflamm. Bowel Dis. 18:1777. Xu, W. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9511. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096. Li, J. et al. (2004) Int. Immunopharmacol. 4:693. Wei, C.-C. et al. (2003) Genes Immun. 4:204. Weber, G.F. et al. (2007) Infect. Immun. 75:1690. Whittington, H.A. et al. (2004) Am. J. Respir. Cell Mol. Biol. 31:220. Lecart, S. et al. (2002) Int. Immunol. 14:1351. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577. Wolk, K. et al. (2007) J. Immunol. 178:5973. Huber, S. et al. (2012) Nature 491:259. Martin, J.C.J. et al. (2014) Mucosal Immunol. 7:101. Dumoutier, L. et al. (2001) J. Immunol. 166:7090.
Long Name	Interleukin 22 Binding Protein
Entrez Gene IDs	116379 (Human); 237310 (Mouse)
Alternate Names	CRF2-10, CRF2-S1IL22BP, CRF2-X, Cytokine receptor class-II member 10, Cytokine receptor family 2 member 10, Cytokine receptor family type 2, soluble 1, IL-22 RA2, IL-22 receptor subunit alpha-2, IL-22BP, IL-22BPCRF2X, IL-22R-alpha-2, IL-22RA2, IL22BP, IL22RA2, Interleukin-22-binding protein, MGC150509, MGC150510, class II cytokine receptor, interleukin 22 receptor, alpha 2, interleukin 22-binding protein, interleukin-22 receptor subunit alpha-2, zcytoR16

Citations for Recombinant Human IL-22BP Fc Chimera Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-22BP Fc Chimera Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Wang et al. (2017), IL-22 Increases Permeability of Intestinal Epithelial Tight Junctions by Enhancing Claudin-2 Expression J. Immunol., 0(0).
PMID: 28939759
Species: Human
Sample Types: Serum
Applications: ELISA (Standard)

Perriard G et al. (2015), Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes. J Neuroinflammation, 12(0):119.
PMID: 26077779
Species: Human
Sample Types: Buffer
Applications: Bioassay

Witte K et al. (2009), Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines. Genes Immun., 10(8):702-14.
PMID: 19798076
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wolk K et al. (2004), IL-22 increases the innate immunity of tissues. Immunity, 21(2):241-54.
PMID: 15308104
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Donnelly RP et al. (2004), The expanded family of class II cytokines that share the IL-10 receptor-2 (IL-10R2) chain. J. Leukoc. Biol., 76(2):314-21.
PMID: 15123776

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