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Recombinant Human IL-20R alpha Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1176-IR

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1176-IR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-20 R alpha protein
Human IL-20 R alpha
(Val30 - Lys250)
Accession # Q9UHF4
DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val30

Predicted Molecular Mass

52 kDa (monomer)

SDS-PAGE

70-85 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rhIL-20 Ra/Fc Chimera at 1 µg/mL (100 µL/well) can bind rhIL-26 with a linear range of 0.3-20 ng/mL.

Formulation, Preparation and Storage

1176-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-20R alpha

IL-20 receptor alpha (IL-20 R alpha), also named IL-20 R1, CRF2-8, and ZCYTOR7, belongs to the class II cytokine receptor family, which includes 12 members. These receptors are characterized by the patterns of conserved amino acid (aa) residues in their extracellular domains, which are composed of tandem fibronectin type III domains (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1).

The gene for human IL-20 R alpha is mapped to chromosome 6 and encodes a 553 aa glycoprotein with a 29 aa signal peptide, a 221 aa extracellular domain, a 24 aa transmembrane region and a 279 aa intracellular domain (2). IL-20 R alpha is widely expressed and is detected at high levels in multiple tissues including skin, testis, heart, placenta, salivary gland and prostate gland (1). The expression of IL-20 R alpha, together with that of IL-20 R beta, is upregulated in psoriatic skin lesions on keratinocytes, immune cells, and endothelial cells (1, 2).

IL-20 R alpha heterodimerizes with IL-20 R beta to form the functional receptor that mediates IL-19, IL-20 and IL-24 signals (3, 4). IL-20 R alpha also heterodimerizes with IL-10 R beta to form the functional receptor complex for IL-26 (5). Binding of these IL-10 family class II cytokines to their functional receptors induces activation of the JAK-STAT signal transduction pathway. At low ligand concentrations, STAT3 has been shown to be the predominant STAT proteins activated through either complexes (3 - 5).

References

  1. Kotenko, S.V. (2003) Cytokine & Growth Factor Reviews 13:223.
  2. Xie, M.H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2001) J. Immunol. 167:3534.
  4. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517s.
  5. Sheikh. F. et al. (2004) J. Immunol. 172:2006.

Long Name

Interleukin 20 Receptor alpha

Alternate Names

CRF2-8, IL-20 R alpha, IL-20R1, IL-20Ra, IL20R alpha, IL20RA, zcytor7

Entrez Gene IDs

53832 (Human); 237313 (Mouse)

Gene Symbol

IL20RA

UniProt

Additional IL-20R alpha Products

Product Documents for Recombinant Human IL-20R alpha Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-20R alpha Fc Chimera Protein, CF

For research use only

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