Product Specifications for Recombinant Human IL-18 R alpha/IL-1 R5 His-tag, CF
Chinese Hamster Ovary cell line, CHO-derived human IL-18 R alpha/IL-1 R5 protein Glu20-Arg329, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Glu20 & Cys22
Predicted Molecular Mass
57-68 kDa, under reducing conditions.
Measured by its ability to inhibit the Recombinant Human IL-18/IL-1F4 Catalog # 9124-IL) induced response on KG-1 human acute myelogenous leukemia cells in the presence of Recombinant Human IL-18 R beta /IL-1 R7 Fc Chimera (Catalog # 9124-IL). The ED50 for this effect is 0.400-4.00 μg/mL.
Scientific Data Examples for Recombinant Human IL-18 R alpha/IL-1 R5 His-tag, CF
Recombinant Human IL‑18 R alpha /IL‑1 R5 His-tag Protein Bioactivity.
Recombinant Human IL‑18 R alpha /IL‑1 R5 His-tag Protein (Catalog # 11179-LR) inhibits the Recombinant Human IL‑18/IL‑1F4 (9124-IL) induced response on KG‑1 human acute myelogenous leukemia cells in the presence of Recombinant Human IL‑18 R beta /IL-1 R7 Fc Chimera (118-AP). The ED50 for this effect is 0.400-4.00 μg/mL.
Recombinant Human IL‑18 R alpha /IL‑1 R5 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human IL‑18 R alpha /IL‑1 R5 His-tag Protein (Catalog # 11179-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57-68- kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5
IL-18 Receptor alpha (IL-18 Ra), also known as IL-18 R1, IL-1 R5, and IL-1 Rrp, is a transmembrane component of the IL-18 Receptor (1). It associates with IL‑18 Rb/IL‑1 R7 to form the high affinity receptor complex for IL-18 and confer cellular responsiveness to IL-18 (2, 3). IL-18 is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. Mature human IL-18 Ra consists of a 308 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 191 aa cytoplasmic domain (4). Within the ECD, human IL-18 Ra shares 56% aa sequence identity with mouse and rat IL-18 Ra. IL-18 Ra is widely expressed on epithelial cells as well as on B cells, NK cells, CD4+ and CD8+ T cells, and dendritic cells (5-8). Signaling through the IL-18 receptor promotes a Th1 biased immune response and NK cell cytolytic activity (6, 7, 9-11). It also contributes to cigarette smoke-induced airway inflammation and epithelial damage (12).
Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
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