Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF

Name: Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF
Brand: R&D Systems
SKU: 816-LR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 816-LR

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	Q13478
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF

Source

Mouse myeloma cell line, NS0-derived human IL-18 R alpha/IL-1 R5 protein

Human IL-18 R alpha (Glu20 - Arg329) Accession # Q13478	IEGRDID	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Glu20

Predicted Molecular Mass

63 kDa (monomer)

SDS-PAGE

95-105 kDa, reducing conditions

Activity

Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL‑18 R beta /IL‑1 R7 Fc Chimera (Catalog # 118-AP). Approximately 5‑15 µg/mL of rhIL-18 Ra/Fc will inhibit 50% of the biological response due to 40 ng/mL of rhIL-18 in the presence of 30 µg/mL of rhIL-18 R beta (AcPL)/Fc Chimera.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

816-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

Item	Value
References	Parnet, P. et al. (1996) J. Biol. Chem. 271:3967. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name	Interleukin 18 Receptor alpha
Entrez Gene IDs	8809 (Human); 16182 (Mouse)
Alternate Names	CD218a, IL-1 R5, IL-1 Rrp, IL-18 R alpha, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Citations for Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF include:

Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Takei S et al. (2011), Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. Arthritis Res. Ther., 13(2):R52.
PMID: 21435242
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Nazarian SH et al. (2007), Yaba monkey tumor virus encodes a functional inhibitor of interleukin-18. J. Virol., 82(1):522-8.
PMID: 17959666

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