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Recombinant Human IL-17RA/IL-17R His-tag Protein, CF

Catalog # 11234-IR | R&D Systems, Inc. a Bio-Techne Brand
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11234-IR-100

Key Product Details

Source

CHO

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-17RA/IL-17R protein
Leu33-Trp320, with a C-terminal 6-His tag.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu33

Predicted Molecular Mass

34 kDa

SDS-PAGE

60-68 kDa, under reducing conditions.

Activity

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050‑1.00 µg/mL in the presence of 10.0 ng/mL recombinant human IL‑17 (Catalog # 7955-IL).

Scientific Data Images for Recombinant Human IL-17RA/IL-17R His-tag Protein, CF

Recombinant Human IL-17RA/IL-17R His-tag Protein Bioactivity.

Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.050‑1.00 µg/mL in the presence of 10.0 ng/mL Recombinant Human IL‑17A (7955-IL).

Recombinant Human IL-17RA/IL-17R His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-17RA/IL-17R His-tag Protein (Catalog # 11234-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-68 kDa.

Formulation, Preparation and Storage

11234-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Background: IL-17RA/IL-17R

Interleukin 17 (also known as CTLA‑8) is a T cell‑expressed pleotropic cytokine. IL‑17 binds to IL‑17 receptor (IL‑17 R) which shares no homology with any known family of receptors. While the expression of IL‑17 is restricted to activated T cells, the IL‑17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The human IL‑17 R gene was localized to chromosome 22. Human IL‑17 R is an 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy‑proximal transmembrane domain, and a 525 aa cytoplasmic tail. The aa sequence of human IL‑17 R is 69% identical to the mouse IL‑17 R. The signaling events of IL‑17 includes activation of NF‑ kappaB and JNK, and require TNF receptor‑associated factors 6 (TRAF6) in the signaling pathway.

References

  1. Yao, Z. et al. (1997) Cytokine 9:794.
  2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Long Name

Interleukin 17 Receptor

Alternate Names

CD217, Cdw217, IL-17 R, IL-17 RA, IL17RA

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Gene Symbol

IL17RA

UniProt

Additional IL-17RA/IL-17R Products

Product Documents for Recombinant Human IL-17RA/IL-17R His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-17RA/IL-17R His-tag Protein, CF

For research use only

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