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Recombinant Human IL-17C Protein

Bio-Techne includes R&D Systems | Catalog # 1234-IL

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Carrier Free
1234-IL-025/CF

Catalog #
Availability
Size / Price
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With Carrier
1234-IL-025

Key Product Details

Source

E. coli

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

E. coli-derived human IL-17C protein
His19-Val197, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

19.8 kDa (monomer)

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-17 RE Fc Chimera (Catalog # 8358-MR) is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human IL-17C that produces 50% of the optimal binding response is 1.5-7.5 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1234-IL
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1234-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17C

Interleukin‑17C (IL‑17C) is a 15‑20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL‑17C shares 79% and 76% amino acid sequence identity with mouse and rat IL‑17C, respectively (3). IL‑17C binds to IL‑17 RE with high affinity and to IL‑17 RA with low affinity (4, 5). These two receptor chains can associate into a heterodimeric receptor for IL‑17C (4‑6). IL‑17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma/ delta T cells, while IL‑17 RA is widely expressed (4, 5). IL‑17 RE is required for mediating the pro‑inflammatory and homeostatic actions of IL‑17C in the skin and mucosa (1, 2). IL‑17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells (4, 6‑9). It is up‑regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL‑17 RE is reciprocally down‑regulated in psoriatic lesions (10). The interaction of IL‑17C with IL‑17 RE promotes mucosal immunity through the induction of anti‑bacterial peptides and pro‑inflammatory cytokines and chemokines (4, 6, 8, 9). IL‑17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL‑17C is additionally up‑regulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL‑17A, IL‑17F, IL‑22, CCR6, and CCL20 (5).

References

  1. Pappu, R. et al. (2012) Trends Immunol. 33:343.
  2. Rubino, S.J. et al. (2012) Trends Immunol. 33:112.
  3. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
  4. Ramirez-Carrozzi, V. et al. (2011) Nat. Immunol. 12:1159.
  5. Chang, S.H. et al. (2011) Immunity 35:611.
  6. Song, X. et al. (2011) Nat. Immunol. 12:1151.
  7. Pfeifer, P. et al. (2012) Am. J. Respir. Cell Mol. Biol. 48:415.
  8. Johnston, A. et al. (2013) J. Immunol. 190:2252.
  9. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
  10. Johansen, C. et al. (2009) Br. J. Dermatol. 160:319.
  11. Reynolds, J.M. et al. (2012) J. Immunol. 189:4226.

Long Name

Interleukin 17C

Alternate Names

Cytokine CX2, IL17C

Entrez Gene IDs

27189 (Human); 234836 (Mouse)

Gene Symbol

IL17C

UniProt

Additional IL-17C Products

Product Documents for Recombinant Human IL-17C Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-17C Protein

For research use only

Citations for Recombinant Human IL-17C Protein (4)

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    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/27/2020

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