What are the diferences between Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB) and Recombinant Human IL-17A (Human Cell-expressed) Protein (Catalog # 7955-IL)?

Name: Recombinant Human IL-17A Protein, CF
Brand: R&D Systems
SKU: 317-ILB
Rating: 5 (4 reviews)

There are multiple differences between Catalog # 317-ILB and 7955-IL. 317-ILB is raised in E. coli, while 7955-IL is raised in Human Embryonic Kidney (HEK293) cells. Human Cell-expressed 7955-IL has a lower endotoxin specification (<0.01 EU per 1 μg of the protein by the LAL method) compared to E. coli-expressed 317-ILB (<0.10 EU per 1 μg of the protein). Human Cell-expressed 7955-IL exhibits greater activity in the CXCL1/GRO alpha secretion assay in HT‑29 human colon adenocarcinoma cells (0.12 - 1.2 ng/mL) compared to E. coli-expressed 317-ILB (0.4-4.0 ng/mL).

Recombinant Human IL-17A Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 317-ILB

Citations (87)

(4)

Product Datasheet / COA / SDS

New Version Available - 7955-IL/CF

New rh IL-17A Available! ~4 fold better activity; HEK293 expressed; Lower endotoxin specification!

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	Q16552
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IL-17A Protein, CF

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Product Specifications for Recombinant Human IL-17A Protein, CF

Source	E. coli-derived human IL-17/IL-17A protein Ile20-Ala155, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	16 kDa (monomer)
Activity	Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.4-4 ng/mL. Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 1.5-7.5 ng/mL.

Scientific Data Examples for Recombinant Human IL-17A Protein, CF

	Recombinant Human IL-17A Protein Bioactivity Recombinant Human IL-17 (Catalog # 317-ILB) induces CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.4-4 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

317-ILB

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-17/IL-17A

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9‑12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9‑11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15).
IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

Item	Value
References	Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479. Yao, Z. et al. (1995) J. Immunol. 155:5483. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593. Chang, S.H. and C. Dong (2007) Cell Res. 17:435. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447. Wright, J.F. et al. (2008) J. Immunol. 181:2799. Rong, Z. et al. (2009) Cell Res. 19:208. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271. Liang, S.C. et al. (2007) J. Immunol. 179:7791. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755. Hsu, H. et al. (2008) Nat. Immunol. 9:166. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857. Wang, L. et al. (2009) J. Exp. Med. 206:1457. Kryczek, I. et al. (2009) Blood 114:357.
Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names	CTLA-8, CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8, IL17, IL17A, IL17interleukin-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A

Citations for Recombinant Human IL-17A Protein, CF (87)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-17A Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

K Jamal Jame et al. (2021), Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD International Journal of Molecular Sciences, 22(13).
PMID: 34281240
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

W Soonthornc et al. (2021), MicroRNA-378a-3p is overexpressed in psoriasis and modulates cell cycle arrest in keratinocytes via targeting BMP2 gene Scientific Reports, 11(1):14186.
PMID: 34244572
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

P Ebner-Peki et al. (2021), Self-assembly of differentiated progenitor cells facilitates spheroid human skin organoid formation and planar skin regeneration Theranostics, 11(17):8430-8447.
PMID: 34373751
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

S Shao et al. (2021), Phospholipase A2 enzymes represent a shared pathogenic pathway in psoriasis and pityriasis rubra pilaris JCI Insight, 0(0).
PMID: 34491907
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Lu et al. (2020), NADPH oxidase 1 is highly expressed in human large and small bowel cancers PLoS ONE, 15(5):e0233208.
PMID: 32428030
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

RA Lu et al. (2020), Inhibiting Airway Smooth Muscle Contraction Using Pitavastatin: A Role for the Mevalonate Pathway in Regulating Cytoskeletal Proteins Front Pharmacol, 11(0):469.
PMID: 32435188
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

L Mercurio et al. (2020), Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21 Aging (Albany NY), 12(8):6823-6851.
PMID: 32302288
Species: Human
Sample Types: Reference Standard

L Gorchs et al. (2020), The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity Sci Rep, 10(1):17444.
PMID: 33060625
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Yan et al. (2020), T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation Cell Rep, 31(13):107820.
PMID: 32610123
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Qin et al. (2019), The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 117(0):84-90.
PMID: 30831444
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Wu et al. (2019), IL-4 and IL-17A Cooperatively Promote Hydrogen Peroxide Production, Oxidative DNA Damage, and Upregulation of Dual Oxidase 2 in Human Colon and Pancreatic Cancer Cells J. Immunol., 0(0).
PMID: 31548328
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Merriman et al. (2019), Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress J. Biol. Chem., 0(0).
PMID: 31591269
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Standard

JC Chamcheu et al. (2019), Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models Cells, 8(9).
PMID: 31540162
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

D Deli? et al. (2019), Integrated microRNA/mRNA expression profiling of the skin of psoriasis patients J. Dermatol. Sci., 0(0).
PMID: 31843230
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

X Xu et al. (2019), The Link between Type III Reg and STAT3-Associated Cytokines in Inflamed Colonic Tissues Mediators Inflamm., 2019(0):7859460.
PMID: 31780871
Species: Rabbit
Sample Types: Complex Sample Type
Applications: In Vivo

M Croes et al. (2018), Interleukin 17 enhances bone morphogenetic protein-2-induced ectopic bone formation Sci Rep, 8(1):7269.
PMID: 29740080
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Mercurio et al. (2018), IL-38 has an anti-inflammatory action in psoriasis and its expression correlates with disease severity and therapeutic response to anti-IL-17A treatment Cell Death Dis, 9(11):1104.
PMID: 30377293
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Rutkowska et al. (2018), EBI2 regulates pro-inflammatory signalling and cytokine release in astrocytes Neuropharmacology, 133(0):121-128.
PMID: 29374507
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Wang et al. (2017), IL-22 Increases Permeability of Intestinal Epithelial Tight Junctions by Enhancing Claudin-2 Expression J. Immunol., 0(0).
PMID: 28939759
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Carretta et al. (2017), BRD3/4 inhibition and FLT3-ligand deprivation target pathways that are essential for the survival of human MLL-AF9+ leukemic cells PLoS ONE, 12(12):e0189102.
PMID: 29240787
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SL Fossum et al. (2017), Ets Homologous Factor (EHF) has Critical Roles in Epithelial Dysfunction in Airway Disease J. Biol. Chem., 0(0).
PMID: 28461336
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KA Cho et al. (2017), Th17 cell-mediated immune responses promote mast cell proliferation by triggering stem cell factor in keratinocytes Biochem. Biophys. Res. Commun., 0(0).
PMID: 28456630
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

BF Wang et al. (2017), Interferon-? induced insufficient autophagy contributes to p62-dependent apoptosis of epithelial cells in chronic rhinosinusitis with nasal polyps Allergy, 0(0).
PMID: 28258963
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JS Ainscough et al. (2017), Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36? Proc. Natl. Acad. Sci. U.S.A, 114(13):E2748-E2757.
PMID: 28289191
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells Sci Rep, 6(0):36795.
PMID: 27827428
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Qingshui Huang (2016), Interleukin-17A-Induced Epithelial-Mesenchymal Transition of Human Intrahepatic Biliary Epithelial Cells: Implications for Primary Biliary Cirrhosis Tohoku J. Exp. Med, 240(4):269-275.
PMID: 27916760
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

TM Garcia-Bat et al. (2016), Enhanced Cytotoxic CD8 T Cell Priming Using Dendritic Cell-Expressing Human Papillomavirus-16 E6/E7-p16INK4 Fusion Protein with Sequenced Anti-Programmed Death-1 J. Immunol, 196(6):2870-8.
PMID: 26851223
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Hiroaki Yamazaki (2016), A Novel PEGylation Method for Improving the Pharmacokinetic Properties of Anti-Interleukin-17A RNA Aptamers Nucleic Acid Ther, 0(0).
PMID: 27827561
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA (Standard)

Dobrina N Hull (2016), Increase in circulating Th17 cells during anti-TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis Arthritis Res. Ther, 18(1):303.
PMID: 28010726
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Robert Sabat (2016), MMP8 Is Increased in Lesions and Blood of Acne Inversa Patients: A Potential Link to Skin Destruction and Metabolic Alterations Mediators Inflamm., 2016(0):4097574.
PMID: 27843200
Species: Complex Species Category
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)

(2016), Inhibiting complex IL-17A and IL-17RA interactions with a linear peptide Sci Rep, 6(0):26071.
PMID: 27184415
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Banas M et al. (2015), The expression and regulation of chemerin in the epidermis. PLoS ONE, 10(2):e0117830.
PMID: 25659101
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ling Y et al. (2015), Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis. J Exp Med, 212(5):619-31.
PMID: 25918342
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay

Chiricozzi et al. (2014), IL-17 induces an expanded range of downstream genes in reconstituted human epidermis model. PLoS ONE, 9(2):e90284.
PMID: 24587313
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ardeljan et al. (2014), Interleukin-17 retinotoxicity is prevented by gene transfer of a soluble interleukin-17 receptor acting as a cytokine blocker: implications for age-related macular degeneration. PLoS ONE, 9(4):e95900.
PMID: 24780906
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wang Y et al. (2014), Interleukin 17A promotes gastric cancer invasiveness via NF-kappaB mediated matrix metalloproteinases 2 and 9 expression. PLoS ONE, 9(6):e96678.
PMID: 24905806
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wu L et al. (2014), The differential regulation of human ACT1 isoforms by Hsp90 in IL-17 signaling. J Immunol, 193(4):1590-9.
PMID: 25024377
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rabeony H et al. (2014), Inhibition of keratinocyte differentiation by the synergistic effect of IL-17A, IL-22, IL-1alpha, TNFalpha and oncostatin M. PLoS ONE, 9(7):e101937.
PMID: 25010647
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Xia et al. (2014), Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway. PLoS ONE, 9(6):e98915.
PMID: 24892823
MacLeod A et al. (2014), Skin-resident T cells sense ultraviolet radiation-induced injury and contribute to DNA repair. J Immunol, 192(12):5695-702.
PMID: 24808367
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Adams et al. (2014), IL-17A induces Pendrin expression and chloride-bicarbonate exchange in human bronchial epithelial cells. PLoS ONE, 9(8):e103263.
PMID: 25141009
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zizzo G et al. (2013), IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids. J Immunol, 190(10):5237-46.
PMID: 23596310
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Johnston A et al. (2013), Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis. Hum Mol Genet, 22(9):1807-15.
PMID: 23376980
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Truchetet M et al. (2013), Interleukin-17A+ cell counts are increased in systemic sclerosis skin and their number is inversely correlated with the extent of skin involvement. Arthritis Rheum, 65(5):1347-56.
PMID: 23335253
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Chen Z et al. (2013), The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis. J Immunol, 190(10):5256-66.
PMID: 23606539
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gordon K et al. (2013), Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis. J Invest Dermatol, 133(3):712-22.
PMID: 23190894
Species: Human
Sample Types: Plasma
Applications: Bioassay

Rosen L et al. (2013), Anti-GM-CSF autoantibodies in patients with cryptococcal meningitis. J Immunol, 190(8):3959-66.
PMID: 23509356
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wilson TR et al. (2012), Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 487(7408):505-9.
PMID: 22763448
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Corvaisier M et al. (2012), IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 10(9):e1001395.
PMID: 23055831
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Ramirez-Carrozzi V et al. (2011), IL-17C regulates the innate immune function of epithelial cells in an autocrine manner. Nat. Immunol., 12(12):1159-66.
PMID: 21993848
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Liu Y et al. (2011), IL-17A and TNF-alpha Exert Synergistic Effects on Expression of CXCL5 by Alveolar Type II Cells In Vivo and In Vitro. J. Immunol., 186(5):3197-205.
PMID: 21282514
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Johnston A et al. (2011), IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression. J. Immunol., 186(4):2613-22.
PMID: 21242515
Heo YJ et al. (2010), IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients. Immunol. Lett., 127(2):150-6.
PMID: 19895848
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Guilloteau K et al. (2010), Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis. J. Immunol., 184(0):5263-70.
PMID: 20335534
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Gudjonsson JE et al. (2010), Evidence for altered Wnt signaling in psoriatic skin. J. Invest. Dermatol., 130(7):1849-59.
PMID: 20376066
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Nishida A et al. (2010), Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts. Gut, 59(4):531-41.
PMID: 19996325
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Pennino D et al. (2010), IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. J. Immunol., 184(9):4880-8.
PMID: 20357258
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Xu G et al. (2010), Opposing roles of IL-17A and IL-25 in the regulation of TSLP production in human nasal epithelial cells. Allergy, 65(5):581-9.
PMID: 19968632
Species: Human
Sample Types: Serum
Applications: ELISA (Capture)

Kisand K et al. (2010), Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines. J. Exp. Med., 207(2):299-308.
PMID: 20123959
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Watanabe H et al. (2009), Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis. J. Invest. Dermatol., 129(3):650-6.
PMID: 18830271
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Tohyama M et al. (2009), IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression. Eur. J. Immunol., 39(10):2779-88.
PMID: 19731362
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wolk K et al. (2009), The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis. Eur. J. Immunol., 39(12):3570-81.
PMID: 19830738
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zrioual S et al. (2009), Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes. J. Immunol., 182(0):3112-20.
PMID: 19234208
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shahrara S et al. (2009), IL-17 induces monocyte migration in rheumatoid arthritis. J. Immunol., 182(6):3884-91.
PMID: 19265168
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Amiable N et al. (2009), Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts. Bone, 44(6):1143-50.
PMID: 19264156
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kinoshita H et al. (2009), Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J. Allergy Clin. Immunol., 123(1):179-86.
PMID: 19056108
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shan M et al. (2009), Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema. Sci Transl Med, 1(4):4ra10.
PMID: 20368170
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rahmoun M et al. (2009), Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils. J. Invest. Dermatol., 129(3):671-81.
PMID: 18843289
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Eid RE et al. (2009), Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells. Circulation, 119(10):1424-32.
PMID: 19255340
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Nishida A et al. (2009), Interleukin-32 expression in the pancreas. J. Biol. Chem., 284(26):17868-76.
PMID: 19386602
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Eyerich K et al. (2009), IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. J. Allergy Clin. Immunol., 123(1):59-66.e4.
PMID: 19056110
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Duluc D et al. (2009), Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 125(2):367-73.
PMID: 19378341
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kato A et al. (2008), Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps. J. Allergy Clin. Immunol., 121(6):1385-92, 1392.
PMID: 18410958
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rickel EA et al. (2008), Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol., 181(6):4299-310.
PMID: 18768888
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Guttman-Yassky E et al. (2008), Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis. J. Immunol., 181(10):7420-7.
PMID: 18981165
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kwan Tat S et al. (2008), Activation of the receptor EphB4 by its specific ligand ephrin B2 in human osteoarthritic subchondral bone osteoblasts. Arthritis Rheum., 58(12):3820-30.
PMID: 19035475
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Zrioual S et al. (2008), IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood. J. Immunol., 180(1):655-63.
PMID: 18097068
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Aujla SJ et al. (2008), IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia. Nat. Med., 14(3):275-81.
PMID: 18264110
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Wright JF et al. (2008), The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J. Immunol., 181(4):2799-805.
PMID: 18684971
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Shioya M et al. (2007), Epithelial overexpression of interleukin-32alpha in inflammatory bowel disease. Clin. Exp. Immunol., 149(3):480-6.
PMID: 17590175
Species: N/A
Sample Types: N/A
Applications: ELISA (Standard)

Todd J et al. (2007), Ultrasensitive flow-based immunoassays using single-molecule counting. Clin. Chem., 53(11):1990-5.
PMID: 17890441
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Witowski J et al. (2007), Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum. Kidney Int., 71(6):514-25.
PMID: 17228364
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wolk K et al. (2007), IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease. J. Immunol., 178(9):5973-81.
PMID: 17442982
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Brechter AB et al. (2007), Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression. Arthritis Rheum., 56(3):910-23.
PMID: 17328065
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Filer A et al. (2006), Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Arthritis Rheum., 54(7):2096-108.
PMID: 16802344
Species: N/A
Sample Types: N/A
Applications: ELISA (Specificity Testing)

Vaknin-Dembinsky A et al. (2006), IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production. J. Immunol., 176(12):7768-74.
PMID: 16751425
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Numasaki M et al. (2005), IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 175(9):6177-89.
PMID: 16237115

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Recombinant Human IL-17A Protein, CF

Name: Zhenping Wang

Application: Used for fibroblasts migration in a modified Boyden chamber with a membrane pore size of 8 μm.

Verified Customer | Posted 09/30/2020
Recombinant Human IL-17A Protein, CF

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Application: Immunoassay Standard

Verified Customer | Posted 08/22/2018
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Application: Immunoassay Standard

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Application: Characterization prior to use.

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FAQs for Recombinant Human IL-17A Protein, CF

What are the diferences between Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB) and Recombinant Human IL-17A (Human Cell-expressed) Protein (Catalog # 7955-IL)?
There are multiple differences between Catalog # 317-ILB and 7955-IL.
- 317-ILB is raised in E. coli, while 7955-IL is raised in Human Embryonic Kidney (HEK293) cells.
- Human Cell-expressed 7955-IL has a lower endotoxin specification (<0.01 EU per 1 μg of the protein by the LAL method) compared to E. coli-expressed 317-ILB (<0.10 EU per 1 μg of the protein).
- Human Cell-expressed 7955-IL exhibits greater activity in the CXCL1/GRO alpha secretion assay in HT‑29 human colon adenocarcinoma cells (0.12 - 1.2 ng/mL) compared to E. coli-expressed 317-ILB (0.4-4.0 ng/mL).

Recombinant Human IL-17A Protein, CF

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Background: IL-17/IL-17A

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Recombinant Human IL-17A Protein, CF

Recombinant Human IL-17A Protein, CF

Recombinant Human IL-17A Protein, CF

Recombinant Human IL-17A Protein, CF

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