Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF

Name: Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 839-B1

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 839-B1

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	P42701
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

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Product Specifications for Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human IL-12 R beta 1 protein

Human IL-12 R beta 1 (Cys24 - Glu540) Accession # P42701	IEGRMD	Human IgG₁ (Pro100 - Lys330)	6-His tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Cys24

Predicted Molecular Mass

84.5 kDa (monomer)

SDS-PAGE

110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Immobilized rhIL-12 R beta 1/Fc Chimera at 5 µg/mL (100 µL/well) can bind rhIL-12 with a linear range of 0.78‑50 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

839-B1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-12 R beta 1

Interleukin 12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL-12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) residue protein with a putative 23 aa residue signal peptide that is cleaved to generate the mature protein with a 522 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 92 aa residue cytoplasmic region. Expression of IL-12 R beta 1 has been detected in activated T cells, NK cells and B cells. The expression of IL-12 R beta 2 is more restricted and appears to be limited to Th2 cells.

Item	Value
References	Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.
Long Name	Interleukin 12 Receptor beta 1
Entrez Gene IDs	3594 (Human); 16161 (Mouse)
Alternate Names	CD212, CD212 antigen, IL-12 R beta 1, IL-12 receptor beta component, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, IL-12R-BETA1, IL-12R-beta-1, IL-12Rb1, IL12R, IL12R beta 1, IL12RB, IL12RB1, MGC34454, interleukin 12 receptor, beta 1, interleukin-12 receptor beta-1 chain, interleukin-12 receptor subunit beta-1

Citations for Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IL-12 R beta 1 Fc Chimera Protein, CF include:

Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture

KJ Roberts et al. (2021), Preclinical development of a bispecific TNFalpha/IL-23 neutralising domain antibody as a novel oral treatment for inflammatory bowel disease Scientific Reports, 11(1):19422.
PMID: 34593832
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay

Yu RY et al. (2010), A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells. J. Immunol., 185(12):7302-8.
PMID: 21076058

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