Recombinant Human IL-10 R alpha Protein, CF

Name: Recombinant Human IL-10 R alpha Protein, CF
Brand: R&D Systems
SKU: 9100-R1

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9100-R1

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Key Product Details

Source:	HEK293
Accession #:	Q13651
Applications:	Bioactivity

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Product Specifications for Recombinant Human IL-10 R alpha Protein, CF

Source	Human embryonic kidney cell, HEK293-derived human IL-10 R alpha protein His22-Asn235
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His22
Predicted Molecular Mass	24 kDa
SDS-PAGE	38-52 kDa, reducing conditions
Activity	Measured by its ability to inhibit IL-10-dependent proliferation of MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED₅₀ for this effect is 0.05‑0.25 µg/mL in the presence of 2 ng/mL of Recombinant Human IL‑10 (Catalog # 217-IL).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9100-R1

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10 R alpha

Interleukin-10 Receptor alpha (IL-10 R alpha ), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL‑10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 R alpha consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 R alpha shares 59% aa sequence identity with mouse and rat IL-10 R alpha.

Item	Value
References	Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. Manzanillo, P. et al. (2015) Trends Immunol. 36:471. Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641. Mannino, M.H. et al. (2015) Cancer Lett. 367:103. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372. Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053. Kotenko, S.V. et al. (1997) EMBO J. 16:5894. Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571. Fernandez, S. et al. (2004) J. Immunol. 172:2613. Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365. Liu, Y. et al. (1994) J. Immunol. 152:1821.
Long Name	Interleukin 10 Receptor alpha
Entrez Gene IDs	3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names	CD210 antigen, CDw210a, HIL-10R, IBD28, IL-10 R alpha, IL-10 receptor subunit alpha, IL-10R subunit 1, IL-10R1, IL-10Ra, IL10R alpha, IL10RA, IL10RIL-10R subunit alpha, Interleukin-10 receptor subunit 1, interleukin 10 receptor, alpha, interleukin-10 receptor alpha chain, interleukin-10 receptor subunit alpha

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Recombinant Human IL-10 R alpha Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human IL-10 R alpha Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: IL-10 R alpha

Citations for Recombinant Human IL-10 R alpha Protein, CF

Product Documents for Recombinant Human IL-10 R alpha Protein, CF

Product Datasheets

COA

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