Recombinant Human IGFBP-6 Protein, CF

Name: Recombinant Human IGFBP-6 Protein, CF
Brand: R&D Systems
SKU: 876-B6

Catalog # 876-B6 | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	NP_002169
Applications:	Bioactivity

View Full Technical Details for Recombinant Human IGFBP-6 Protein, CF

Technical Data
Citations (5) / Reviews

Product Specifications for Recombinant Human IGFBP-6 Protein, CF

Source	Mouse myeloma cell line, NS0-derived human IGFBP-6 protein Ala25-Gly240, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	23 kDa
SDS-PAGE	23 kDa
Activity	Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 0.025-0.15 µg/mL in the presence of 14 ng/mL Recombinant Human IGF‑II (Catalog # 292-G2).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

876-B6

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

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Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-6 cDNA encodes a 240 amino acid (aa) residue precursor protein with a putative 24 aa residue signal peptide that is processed to generate the 216 aa residue mature protein that is O-glycosylated. IGFBP-6 is expressed in ovarian cells, prostatic cells, and fibroblasts. IGFBP-6 is found predominantly in CSF and serum. IGFBP-6 binds preferentially to IGF-II, exhibiting a 2-fold higher affinity for IGF-II than for IGF-I.

Item	Value
References	Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Long Name	Insulin-like Growth Factor Binding Protein 6
Entrez Gene IDs	3489 (Human); 16012 (Mouse)
Alternate Names	IBP-6, IBP6, IGF binding protein 6, IGF-binding protein 6, IGFBP-6, IGFBP6, insulin-like growth factor binding protein 6, insulin-like growth factor-binding protein 6

Citations for Recombinant Human IGFBP-6 Protein, CF (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human IGFBP-6 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Q Chen et al. (2016-10-18), IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker Oncotarget, 2016-17(42):68140-68150.
PMID: 27623076
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Zhu W et al. (2013-11-12), Expression of IGFBP6 in proliferative vitreoretinopathy rat models and its effects on retinal pigment epithelialJ cells. Mol Med Rep, 2013-19(1):33-8.
PMID: 24220750
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Barkho BZ et al. (2006-06-01), Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 2006-015(3):407-21.
PMID: 16846377
Species: Human
Sample Types: N/A
Applications: Enzyme Assay

Cortesio CL et al. (2006-03-03), Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch. Biochem. Biophys., 2006-0449(1):164-70.
PMID: 16554018
Species: Human
Sample Types: Recombinant Protein, Whole Cells
Applications: Bioassay, Enzyme Assay

Nakamura M et al. (2005-08-05), Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Biochem. Biophys. Res. Commun., 2005-0333(3):1011-6.
PMID: 15964556

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Showing 1 - 5 of 5 citations Showing All

Product Documents for Recombinant Human IGFBP-6 Protein, CF

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