Chinese Hamster Ovary cell line, CHO-derived human Hyaluronidase 2/HYAL2 protein Met21-Gly447, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
53-58 kDa, under reducing conditions.
Measured by its ability to digest Cy5-Labeled Hyaluronan (Low MW) to dp1-dp4. The majority of Cy5-Labeled Hyaluronan (Low MW) (2 pmol) is digested by 0.25 μg of Recombinant Human HYAL-2, as measured under the described conditions.
Recombinant Human Hyaluronidase 2/HYAL2 His-tag Protein, CF Scientific Data Examples
Recombinant Human HYAL-2 His-tag Protein (rhHYAL-2) Enzyme Activity Diagram
rhHYAL-2 recognizes repeating subunits of -GlcA-beta 1,3-GlcNAc-beta 1,4- and hydrolyzes high molecular weight hyaluronic acid to intermediate and smaller sized products.
Recombinant Human Hyaluronidase 2/HYAL2 SDS-PAGE
1 μg/lane of rhHYAL-2 (Catalog # 11012-GH) was resolved with SDS-PAGE under reducing (R) conditions and visualized by Silver Staining, showing a band at 55 kDa.
Fluorescent Gel Mobility Shift caused by rhHYAL-2.
Lane 1 contained substrate Cy5-labeled LMW-HA GL401. In the presence of rhHYAL-2, the Hyaluronic Acid was modified to smaller molecular weight products with increasing amounts of rhHYAL-2 from left to right and a mobility shift was observed.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after opening.
Background: Hyaluronidase 2/HYAL2
Human hyaluronidases (HYALs) are a group of five endo-beta -N-acetylhexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20) (1, 2). While HYAL1, HYAL2 and SPAM1 are mainly active on hyaluronan (3, 4, 5), HYAL4 is active on both hyaluronan (6) and chondroitin sulfate (type C and D in particular) (7). Hyaluronan and chondroitin sulfate are abundant extracellular matrix components that have numerous biological functions (8). The backbone of chondroitin sulfate composed of repeating units of 4-GlcA-beta 1,3-GalNAc-beta 1 most closely resembles to that of hyaluronan composed of repeating units of 4-GlcA-beta 1,3-GlcNAc-beta 1. While HYAL1 is a strict lysosomal hyaluronidase and is active when pH ≤ 5.5 (9), HYAL2 is active in a narrow range of pH 5.0 to 5.5 (6) and is attached to cell membrane via GPI linker (10) where they may serve to hydrolyze high molecular weight hyaluronic acid of extracellular matrix to intermediate and small sized products.
Csoka, A.B. et al. (2001) Matrix Biol. 20:499.
Jedrzejas, M. J. and Stern, R. (2005) Proteins 61:227.
Csoka, A.B. et al. (1999) Genomics 60:351.
Lin, Y. et al. (1994) J. Cell Biol.125:1157.
Lepperdinger, G. et al. (2001) Matrix Biol. 20:509.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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