Recombinant Human Hyaluronidase 2/HYAL2 His-tag Protein, CFNew

Name: Recombinant Human Hyaluronidase 2/HYAL2 His-tag Protein, CF
Brand: R&D Systems
SKU: 11012-GH

Catalog # 11012-GH

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Key Product Details

Source:	CHO
Accession #:	Q12891.4
Applications:	Binding Activity

View Full Technical Details for Recombinant Human Hyaluronidase 2/HYAL2 His-tag Protein, CF

Product Specifications

Source	Chinese Hamster Ovary cell line, CHO-derived human Hyaluronidase 2/HYAL2 protein Met21-Gly447, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met21
Predicted Molecular Mass	50 kDa
SDS-PAGE	53-58 kDa, under reducing conditions.
Activity	Measured by its ability to digest Cy5-Labeled Hyaluronan (Low MW) to dp1-dp4. The majority of Cy5-Labeled Hyaluronan (Low MW) (2 pmol) is digested by 0.25 μg of Recombinant Human HYAL-2, as measured under the described conditions.

Recombinant Human Hyaluronidase 2/HYAL2 His-tag Protein, CF Scientific Data Examples

	Recombinant Human HYAL-2 His-tag Protein (rhHYAL-2) Enzyme Activity Diagram rhHYAL-2 recognizes repeating subunits of -GlcA-beta 1,3-GlcNAc-beta 1,4- and hydrolyzes high molecular weight hyaluronic acid to intermediate and smaller sized products.
	Recombinant Human Hyaluronidase 2/HYAL2 SDS-PAGE 1 μg/lane of rhHYAL-2 (Catalog # 11012-GH) was resolved with SDS-PAGE under reducing (R) conditions and visualized by Silver Staining, showing a band at 55 kDa.
	Fluorescent Gel Mobility Shift caused by rhHYAL-2. Lane 1 contained substrate Cy5-labeled LMW-HA GL401. In the presence of rhHYAL-2, the Hyaluronic Acid was modified to smaller molecular weight products with increasing amounts of rhHYAL-2 from left to right and a mobility shift was observed.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11012-GH

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

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Background: Hyaluronidase 2/HYAL2

Human hyaluronidases (HYALs) are a group of five endo-beta -N-acetylhexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20) (1, 2). While HYAL1, HYAL2 and SPAM1 are mainly active on hyaluronan (3, 4, 5), HYAL4 is active on both hyaluronan (6) and chondroitin sulfate (type C and D in particular) (7). Hyaluronan and chondroitin sulfate are abundant extracellular matrix components that have numerous biological functions (8). The backbone of chondroitin sulfate composed of repeating units of 4-GlcA-beta 1,3-GalNAc-beta 1 most closely resembles to that of hyaluronan composed of repeating units of 4-GlcA-beta 1,3-GlcNAc-beta 1. While HYAL1 is a strict lysosomal hyaluronidase and is active when pH ≤ 5.5 (9), HYAL2 is active in a narrow range of pH 5.0 to 5.5 (6) and is attached to cell membrane via GPI linker (10) where they may serve to hydrolyze high molecular weight hyaluronic acid of extracellular matrix to intermediate and small sized products.

Item	Value
References	Csoka, A.B. et al. (2001) Matrix Biol. 20:499. Jedrzejas, M. J. and Stern, R. (2005) Proteins 61:227. Csoka, A.B. et al. (1999) Genomics 60:351. Lin, Y. et al. (1994) J. Cell Biol.125:1157. Lepperdinger, G. et al. (2001) Matrix Biol. 20:509. Wu, Z.L. and Ertelt, J.M. (2021) Glycobiology, cwab061, https://doi.org/10.1093/glycob/cwab061. Kaneiwa, T. et al. (2010) Glycobiology 20:300. Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609. Hofinger, E.S.A. et al. (2007) Glycobiology 17:444. Rai, S.K. et al. (2001). Proc. Natl. Acad. Sci. U.S.A, 98:4443.
Long Name	Hyaluronoglucosaminidase-2
Entrez Gene IDs	8692 (Human)
Alternate Names	EC 3.2.1.35, HYAL-2, Hyaluronidase 2, LUCA-2, LUCA2, LUCA2hyaluronidase-2, Lung carcinoma protein 2, PH-20 homolog, PH20 homolog, hyaluronoglucosaminidase 2, hyaluronoglucosaminidase-2, lysosomal hyaluronidase

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