Recombinant Human Granzyme H Protein, CF

Name: Recombinant Human Granzyme H Protein, CF
Brand: R&D Systems
SKU: 1377-SE
Price: 417 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1377-SE

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	P20718
Structure / Form:	Pro form
Applications:	Enzyme Activity

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Product Specifications for Recombinant Human Granzyme H Protein, CF

Source	Mouse myeloma cell line, NS0-derived human Granzyme H protein Glu19-Leu246, with a C-terminal 10-His tag Accession # P20718
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu19
Predicted Molecular Mass	27 kDa
SDS-PAGE	Multiple bands between 38-40 kDa, reducing conditions
Activity	Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1377-SE

Formulation	Supplied as a 0.2 μm filtered solution in HEPES, NaCl and Brij-35.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

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Background: Granzyme H

Granzyme H is a member of the granzyme family of serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme H’s functions are largely unknown. The more abundant expression of Granzyme H than Granzyme B in NK cells suggests that Granzyme H may complement the pro-apoptotic function of Granzyme B in this cell type (3). Human Granzyme H shows the highest amino acid identity (71%) to mouse Granzyme C (4). Human Granzyme H is synthesized as a precursor (246 residues) with a signal peptide (residues 1-18), a propeptide (residues 19-20) and a mature chain (residues 21-246) (5-7). The pro-enzyme is expressed and purified. After being activated by active cathepsin C, rhGranzyme H cleaves a thioester substrate, which was described previously (8).

Item	Value
References	Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555. Sedelies, K.A. et al. (2004) J. Biol. Chem. 279:26581. Sattar, R. et al. (2003) Biochem. Biophys. Res. Comm. 308:726. Meier, M. et al. (1990) Biochemistry 29:4042. Haddad, P. et al. (1991) Int. Immunol. 3:57. Klein, J.L. et al. (1990) Tissue Antigens 35:220. Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
Entrez Gene IDs	2999 (Human)
Alternate Names	CCP-X, CCP-Xcytotoxic T-lymphocyte-associated serine esterase 1, CGL-2, CGL2, CSP-Ccytotoxin serine protease-C, CTLA1, CTSGL2, CTSGL2granzyme H, Cathepsin G-like 2, Cytotoxic T-lymphocyte proteinase, Cytotoxic serine protease C, EC 3.4.21, EC 3.4.21.-, EC 3.4.21.79, GZMH, Granzyme H, GrzH, granzyme H (cathepsin G-like 2, protein h-CCPX)

Citations for Recombinant Human Granzyme H Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Granzyme H Protein, CF include:

Species: N/A
Sample Types: Protein
Applications: Enzyme Assay

BM Larimer et al. (2017-01-01), Granzyme B PET Imaging as a Predictive Biomarker of Immunotherapy Response Cancer Res., 77(9):2318-2327.
PMID: 28461564
Sample Types: Substrate
Applications: Enzyme Assay

Fellows E et al. (), Natural killer cell-derived human granzyme H induces an alternative, caspase-independent cell-death program. Bloo110(2):544-52.
PMID: 17409270

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