Human embryonic kidney cell, HEK293-derived human GPR158 protein Ala24-Gln411, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ala24 & Ser25
Predicted Molecular Mass
50-70 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Human GPR158 is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neuropilin-1 (Catalog # 3870-N1) with an ED50 of 0.8-4.8 μg/mL
Recombinant Human GPR158 His-tag Protein, CF Scientific Data Examples
Recombinant Human GPR158 His-tag Protein Binding Activity
When Recombinant Human GPR158 (Catalog # 10286-GP) is coated at 1 µg/mL, 100 µL/well, Recombinant Human Neuropilin-1 (Catalog # 3870-N1) binds with an ED50 of 0.8-4 µg/mL.
Recombinant Human GPR158 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Human GPR158 His-tag Protein (Catalog # 10286-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 1 mg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
G-protein coupled receptor 158 (GPR158) is a receptor belonging to the Class C GPCR family. It lacks the extracellular Venus flytrap module characteristic of the known members of that family and instead contains two other elements that are not typical of the class: a calcium-binding EGF-like domain and a leucine repeat region (1, 2). The mature extracellular domain of human GPR158 contains 393 amino acids (aa) and shares 89% identity with both mouse and rat GPR158. GPR158 is expressed at the highest level in the brain, but also in a variety of other tissues including retina, spleen, liver and lung (3). GPR158 was originally identified in functional screens linked with biological stress and has been implicated in the osteocalcin effect on cognitive processes in the brain (4, 5), and glaucoma and cancer in the periphery (4, 6).
Jingami, H. et al. (2003) Curr. Opin. Neurobiol. 13:271.
Bjarnadóttir, T.K et al. (2005) Gene. 362:70.
Orlandi, C. et al. (2012) J. Cell Biol. 197:711.
Itakura, T. et al. (2019) J. Ocul. Pharmacol. Ther. 35:203.
Which Brands are Currently Available on bio-techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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