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Recombinant Human GPR158 His-tag Protein, CF

Catalog # 10286-GP | R&D Systems, Inc. a Bio-Techne Brand
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10286-GP-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human GPR158 protein
Ala24-Gln411, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24 & Ser25

Predicted Molecular Mass

45 kDa

SDS-PAGE

50-70 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human GPR158 is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neuropilin-1 (Catalog # 3870-N1) with an ED50 of 0.8-4.8 μg/mL

Scientific Data Images for Recombinant Human GPR158 His-tag Protein, CF

Recombinant Human GPR158 His-tag Protein Binding Activity

Recombinant Human GPR158 His-tag Protein Binding Activity

When Recombinant Human GPR158 (Catalog # 10286-GP) is coated at 1 µg/mL, 100 µL/well, Recombinant Human Neuropilin-1 (Catalog # 3870-N1) binds with an ED50 of 0.8-4 µg/mL.
Recombinant Human GPR158 His-tag Protein SDS-PAGE

Recombinant Human GPR158 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human GPR158 His-tag Protein (Catalog # 10286-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

Formulation, Preparation and Storage

10286-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR158

G-protein coupled receptor 158 (GPR158) is a receptor belonging to the Class C GPCR family. It lacks the extracellular Venus flytrap module characteristic of the known members of that family and instead contains two other elements that are not typical of the class: a calcium-binding EGF-like domain and a leucine repeat region (1, 2). The mature extracellular domain of human GPR158 contains 393 amino acids (aa) and shares 89% identity with both mouse and rat GPR158. GPR158 is expressed at the highest level in the brain, but also in a variety of other tissues including retina, spleen, liver and lung (3). GPR158 was originally identified in functional screens linked with biological stress and has been implicated in the osteocalcin effect on cognitive processes in the brain (4, 5), and glaucoma and cancer in the periphery (4, 6).

References

  1. Jingami, H. et al. (2003) Curr. Opin. Neurobiol. 13:271.
  2. Bjarnadóttir, T.K et al. (2005) Gene. 362:70.
  3. Orlandi, C. et al. (2012) J. Cell Biol. 197:711.
  4. Itakura, T. et al. (2019) J. Ocul. Pharmacol. Ther. 35:203.
  5. Khrimian, L. et al. (2017) J. Exp. Med. 214:2859.
  6. Fenner, A. (2015) Nat. Rev. Urol. 12:182.

Alternate Names

KIAA1136, RP11-59G22.1

Entrez Gene IDs

57512 (Human); 241263 (Mouse); 291352 (Rat)

Gene Symbol

GPR158

UniProt

Product Documents for Recombinant Human GPR158 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human GPR158 His-tag Protein, CF

For research use only

Reconstitution Calculator

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