Product Specifications for Recombinant Human GLP-1R His-tag Protein, CF
Chinese Hamster Ovary cell line, CHO-derived human GLP-1R protein Ala21-Glu139, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Ala2 & Arg24
Predicted Molecular Mass
15 kDa & 16 kDa
40-50 kDa, under reducing conditions.
Measured by its binding ability in a functional ELISA. When GLP-1 peptide (Tocris, Catalog # 5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag binds with an ED50 of 5.00-30.0 ng/mL.
Scientific Data Examples for Recombinant Human GLP-1R His-tag Protein, CF
Recombinant Human GLP-1R His-tag Protein Binding Activity.
When GLP-1 peptide (5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) binds with an ED50 of 5.00-30.0 ng/mL.
Recombinant Human GLP-1R His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-50 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Glucagon-like Peptide 1 Receptor (GLP1R) is a member of the Class B or secretin receptor-like family of G protein-coupled receptors (GPCRs) (1-3). Activation of GLP1R signaling regulates secretion of insulin from pancreatic beta -cells in a glucose-dependent manner (4). Human GLP1R consists of an N-terminal, extracellular domain (ECD) containing 6 conserved cysteine residues and a transmembrane domain with seven membrane-spanning alpha-helices, characteristic of GPCRs (1, 3, 5). The mature ECD of human GLP1R shares 87% and 86% amino acid identity with mouse and rat GLP1R, respectively. GLP1R is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach and it is a receptor for Glucagon-like Peptide, a regulator of insulin secretion by pancreatic beta cells (2, 5, 6). GLP1R agonists, including GLP1, Boc5, and Cpd1, have been targeted for research and used as type 2 diabetes treatments (6, 7). GLP1R is also expressed in the brain where it is involved in the control of appetite (7).
Ma, H. et al. (2020) Cell Research. 30:1140.
Song, G. et al. (2017) Nature. 546:312.
Hollenstein, K. et al. (2013) Trends Pharmacol Sci. 35:12.
Drucker, D.J. et al. (1987) PNAS. 84(10):3434.
Donnelly, D. (2012) Br. J. Pharmacol. 166:27.
Simo, R. and Hernandez, C. (2017) Diabetes. 66:1453.
Holst, J.J. (2004) Expert Opinion on Emerging Drugs. 9:155.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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